Chlorination‐Driven BODIPY Fluorescent Probes for the Selective Monitoring of Myeloperoxidase Activity in Cells and Inflamed Mouse Models
- 1Department of Chemistry and Research Institute of Basic Sciences Kyung Hee University Seoul South Korea
- 2Department of Life Science Chung‐Ang University Seoul South Korea
- 3School of Pharmacy Chung‐Ang University Seoul South Korea
Abstract
Myeloperoxidase (MPO) generates hypochlorous acid (HOCl), a major oxidant in inflammatory biology and an increasingly recognized pathogenic mediator and therapeutic target in oxidative and inflammatory diseases. However, selective detection of MPO activity remains challenging because most HOCl‐responsive fluorescent probes operate through nonspecific oxidative mechanisms, rendering them vulnerable to interference from competing reactive oxygen and nitrogen species. Here, we present an MPO‐reporting fluorescent platform based on a meso‐carboxamide‐substituted 3,5‐dimethyl BODIPY scaffold that exploits the electrophilic chlorinating reactivity of MPO‐derived HOCl. The lead probe undergoes rapid chlorination at the 2,6‐positions, generating highly emissive products with ca. 40 nm bathochromic shifts and overcoming the minimal spectral responses and fluorescence quenching that have limited earlier chlorination‐based designs. The modular meso‐carboxamide architecture further allows facile incorporation of targeting motifs without compromising probe performance. This platform enables quantitative MPO assays, cellular imaging of exogenous and endogenous MPO‐derived HOCl, discrimination between cancer and normal cells based on redox differences, and noninvasive visualization of MPO‐driven inflammation in a mouse model of atopic dermatitis. These results establish electrophilic chlorination as a viable design strategy for selective MPO‐responsive fluorescent probes and provide a versatile tool for investigating MPO‐associated inflammatory pathophysiology in biological and preclinical disease models.
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BibTeX
@article{cho2026chlorinationdriven,
title = {{Chlorination‐Driven BODIPY Fluorescent Probes for the Selective Monitoring of Myeloperoxidase Activity in Cells and Inflamed Mouse Models}},
author = {Siyoung Cho and Yongyang Luo and Jeehyeon Bae and Youngmi Kim},
journal = {Angewandte Chemie (International Ed. in English)},
year = {2026},
month = sep,
doi = {10.1002/anie.9709775},
url = {https://doi.org/10.1002/anie.9709775},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Chlorination‐Driven BODIPY Fluorescent Probes for the Selective Monitoring of Myeloperoxidase Activity in Cells and Inflamed Mouse Models
AU - Siyoung Cho
AU - Yongyang Luo
AU - Jeehyeon Bae
AU - Youngmi Kim
PY - 2026
DA - 2026/09/16
JO - Angewandte Chemie (International Ed. in English)
DO - 10.1002/anie.9709775
UR - https://doi.org/10.1002/anie.9709775
AB - Myeloperoxidase (MPO) generates hypochlorous acid (HOCl), a major oxidant in inflammatory biology and an increasingly recognized pathogenic mediator and therapeutic target in oxidative and inflammatory diseases. However, selective detection of MPO activity remains challenging because most HOCl‐responsive fluorescent probes operate through nonspecific oxidative mechanisms, rendering them vulnerable to interference from competing reactive oxygen and nitrogen species. Here, we present an MPO‐reporting fluorescent platform based on a meso‐carboxamide‐substituted 3,5‐dimethyl BODIPY scaffold that exploits the electrophilic chlorinating reactivity of MPO‐derived HOCl. The lead probe undergoes rapid chlorination at the 2,6‐positions, generating highly emissive products with ca. 40 nm bathochromic shifts and overcoming the minimal spectral responses and fluorescence quenching that have limited earlier chlorination‐based designs. The modular meso‐carboxamide architecture further allows facile incorporation of targeting motifs without compromising probe performance. This platform enables quantitative MPO assays, cellular imaging of exogenous and endogenous MPO‐derived HOCl, discrimination between cancer and normal cells based on redox differences, and noninvasive visualization of MPO‐driven inflammation in a mouse model of atopic dermatitis. These results establish electrophilic chlorination as a viable design strategy for selective MPO‐responsive fluorescent probes and provide a versatile tool for investigating MPO‐associated inflammatory pathophysiology in biological and preclinical disease models.
SN - 1433-7851
SN - 1521-3773
ER -