Lineage-specific regulation and RNA-associated functions of SATB1 in mature B-cells
Abstract
Special AT-rich binding protein 1 (SATB1) is a nuclear matrix-associated transcription factor that orchestrates higher-order chromatin architecture and gene expression in T and B lymphocytes. While SATB1’s role in T cells is well-established, its regulation and function in B cells remain underexplored. Here, we showed that Satb1 expression in mature B cells is controlled by alternative promoter usage and splicing, with promoters P1 and P3 dynamically switching upon LPS-induced activation, while P2 remains transcriptionally silent. We identified four Satb1 mRNA isoforms, including two novel transcripts ( Δ9 and Δ9Δ11 ), all of which maintain open reading frames. Satb1 transcription decreases upon activation while SATB1 protein content evolves differently, suggesting post-transcriptional regulation. SATB1 forms a high-molecular-weight complex in B cells, and co-immunoprecipitation mass spectrometry reveals RNA-binding proteins as its predominant interactors. These findings specify SATB1 expression kinetics during B-cell activation and reveal its role as a regulator of RNA splicing in B cells.
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BibTeX
@misc{frayssinhes2026lineagespecific,
title = {{Lineage-specific regulation and RNA-associated functions of SATB1 in mature B-cells}},
author = {Jean-Yves Alejandro Frayssinhes and Lya Meriot and Tiffany Marchiol and Emilie Pinault and Thierry Forné and Charalampos Spilianakis and Eric Pinaud and Sandrine Le Noir},
howpublished = {HAL},
year = {2026},
month = jul,
doi = {10.64898/2026.07.22.739998},
url = {https://hal.science/hal-05802449v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - UNPB
TI - Lineage-specific regulation and RNA-associated functions of SATB1 in mature B-cells
AU - Jean-Yves Alejandro Frayssinhes
AU - Lya Meriot
AU - Tiffany Marchiol
AU - Emilie Pinault
AU - Thierry Forné
AU - Charalampos Spilianakis
AU - Eric Pinaud
AU - Sandrine Le Noir
PY - 2026
DA - 2026/07/24
PB - HAL
DO - 10.64898/2026.07.22.739998
UR - https://hal.science/hal-05802449v1
AB - Special AT-rich binding protein 1 (SATB1) is a nuclear matrix-associated transcription factor that orchestrates higher-order chromatin architecture and gene expression in T and B lymphocytes. While SATB1’s role in T cells is well-established, its regulation and function in B cells remain underexplored. Here, we showed that Satb1 expression in mature B cells is controlled by alternative promoter usage and splicing, with promoters P1 and P3 dynamically switching upon LPS-induced activation, while P2 remains transcriptionally silent. We identified four Satb1 mRNA isoforms, including two novel transcripts ( Δ9 and Δ9Δ11 ), all of which maintain open reading frames. Satb1 transcription decreases upon activation while SATB1 protein content evolves differently, suggesting post-transcriptional regulation. SATB1 forms a high-molecular-weight complex in B cells, and co-immunoprecipitation mass spectrometry reveals RNA-binding proteins as its predominant interactors. These findings specify SATB1 expression kinetics during B-cell activation and reveal its role as a regulator of RNA splicing in B cells.
ER -