Decoding radiation-induced transcriptomic signatures of human leukocytes using long-read RNA-seq: clinical and biodosimetric implications
Abstract
Background: Gene expression profiling in radiation-exposed blood is a valuable tool for biodosimetry and clinical research. Evaluating the blood’s transcriptomic radiation response provides insight into absorbed dose, hematotoxicities, and immune reactions. However, detailed analysis using long-read RNA sequencing is currently limited in its diffusion, despite the potential additional insights that could be extracted, including novel isoform discovery and on-the-field gene expression studies, owing to its portability.
Results: In this study, we utilized Oxford Nanopore Technologies’ long-read RNA sequencing on human peripheral blood leukocytes from three healthy donors 6 h after exposure of whole peripheral blood to 4 Gy of X-rays. Compared to sham-irradiated (0 Gy) blood samples, gene-level differential expression analysis identified 117 upregulated and 66 downregulated genes, including canonical DNA damage repair and inflammatory responses. At the transcript level, 102 transcripts were significantly upregulated, and 17 were downregulated, revealing isoform-specific regulation that was not captured at the gene level. Notably, IL32 , which was not detected as significantly changed at the gene level, exhibited significant upregulation of two transcript isoforms (log2 fold-changes of 1.19 and 1.99), while WDR74 , ITM2B , AK2 , and RPS19 displayed changes in transcript usage following irradiation. Leveraging long-read RNA sequencing, we further identified novel candidate isoforms, including protein-coding isoforms of PHPT1 and FBXO22 as well as non-coding candidates. Notably, the FBXO22 isoform showed evidence of altered protein domain architecture relative to the reference isoform.
Conclusions: This study is the first to systematically investigate differential transcript usage in the leukocyte fraction of irradiated human whole blood using long-read RNA sequencing. We confirmed known radiation-response signatures at the gene level and identified transcript-level regulatory events and novel candidate isoforms that can be masked in gene-level analyses and conventional short-read pipelines. These findings demonstrate the potential of long-read RNA sequencing to provide a more detailed view of radiation-induced transcriptomic alterations, with potential applications in biodosimetry and clinical settings.
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BibTeX
@article{salah2026decoding,
title = {{Decoding radiation-induced transcriptomic signatures of human leukocytes using long-read RNA-seq: clinical and biodosimetric implications}},
author = {Ahmed Salah and Heinz Schmidberger and Federico Marini and Sebastian Zahnreich},
journal = {BMC Genomics},
year = {2026},
month = oct,
doi = {10.1186/s12864-026-13475-3},
url = {https://link.springer.com/article/10.1186/s12864-026-13475-3},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Decoding radiation-induced transcriptomic signatures of human leukocytes using long-read RNA-seq: clinical and biodosimetric implications
AU - Ahmed Salah
AU - Heinz Schmidberger
AU - Federico Marini
AU - Sebastian Zahnreich
PY - 2026
DA - 2026/10/17
JO - BMC Genomics
DO - 10.1186/s12864-026-13475-3
UR - https://link.springer.com/article/10.1186/s12864-026-13475-3
AB - Background: Gene expression profiling in radiation-exposed blood is a valuable tool for biodosimetry and clinical research. Evaluating the blood’s transcriptomic radiation response provides insight into absorbed dose, hematotoxicities, and immune reactions. However, detailed analysis using long-read RNA sequencing is currently limited in its diffusion, despite the potential additional insights that could be extracted, including novel isoform discovery and on-the-field gene expression studies, owing to its portability. Results: In this study, we utilized Oxford Nanopore Technologies’ long-read RNA sequencing on human peripheral blood leukocytes from three healthy donors 6 h after exposure of whole peripheral blood to 4 Gy of X-rays. Compared to sham-irradiated (0 Gy) blood samples, gene-level differential expression analysis identified 117 upregulated and 66 downregulated genes, including canonical DNA damage repair and inflammatory responses. At the transcript level, 102 transcripts were significantly upregulated, and 17 were downregulated, revealing isoform-specific regulation that was not captured at the gene level. Notably, IL32 , which was not detected as significantly changed at the gene level, exhibited significant upregulation of two transcript isoforms (log2 fold-changes of 1.19 and 1.99), while WDR74 , ITM2B , AK2 , and RPS19 displayed changes in transcript usage following irradiation. Leveraging long-read RNA sequencing, we further identified novel candidate isoforms, including protein-coding isoforms of PHPT1 and FBXO22 as well as non-coding candidates. Notably, the FBXO22 isoform showed evidence of altered protein domain architecture relative to the reference isoform. Conclusions: This study is the first to systematically investigate differential transcript usage in the leukocyte fraction of irradiated human whole blood using long-read RNA sequencing. We confirmed known radiation-response signatures at the gene level and identified transcript-level regulatory events and novel candidate isoforms that can be masked in gene-level analyses and conventional short-read pipelines. These findings demonstrate the potential of long-read RNA sequencing to provide a more detailed view of radiation-induced transcriptomic alterations, with potential applications in biodosimetry and clinical settings.
SN - 1471-2164
ER -