Development of Supramolecular Calix[4]arene-MCM-Based Nanomaterials Doped with CuNPs: An Inorganic–Organic Hybrid Recyclable Nanoenzyme as a Potent Peroxidase and Oxidase Mimic
- 1J.C. Bose University of Science and Technology, YMCA , , , ,
- 2Indian Institute of Technology Bombay , , , ,
- 3University of Virginia , , , ,
Abstract
An inorganic–organic hybrid nanomaterial, Cu@MCMCal, was synthesized by immobilizing p-tert-butyl calix[4]arene on to mesoporous silica followed by incorporation of CuNPs. The synthesized nanomaterial was characterized extensively using various analytical techniques such as FT-IR, UV–Vis spectroscopy, TEM, PXRD, XPS, Cu LMM Auger, BET, and TGA analyses. The hybrid nanomaterial was evaluated for its catalytic performance toward peroxidase-like activity using 3,3′,5,5′-tetramethylbenzidine (TMB) and o-phenylenediamine (OPD) as a substrate at room temperature at pH 4.5 in acetate buffer and pH 7.4 in PBS buffer, respectively. Enzyme kinetics was performed for both the substrates using the Michaelis–Menten steady-state kinetics and the Lineweaver–Burk plot to determine the catalytic affinity. Based on the kinetic measurements, the values of the Km for TMB and OPD were found to be 0.661 ± 0.143 and 0.606 ± 0.061 mM, respectively. The hybrid nanoenzyme also exhibits the oxidase-like activity using dopamine as a substrate at pH 7.4 in PBS buffer. The Cu@MCMCal nanomaterial acted as a nanoenzyme, efficient for catalysis toward oxidation reactions with better cost effectiveness and improved stability and recyclability as compared to biological enzymes. Therefore, a promising, stable, efficient, and robust nanoenzyme has been development for oxidation reactions.
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BibTeX
@article{goyal2026development,
title = {{Development of Supramolecular Calix[4]arene-MCM-Based Nanomaterials Doped with CuNPs: An Inorganic–Organic Hybrid Recyclable Nanoenzyme as a Potent Peroxidase and Oxidase Mimic}},
author = {Meenakshi Goyal and Anuj Kumar and Pardeep Kumar and Bhawna Uttam},
journal = {ACS Omega},
year = {2026},
month = oct,
doi = {10.1021/acsomega.6c07489},
url = {https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c07489/5453036/Development-of-Supramolecular-Calix-4-arene-MCM},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Development of Supramolecular Calix[4]arene-MCM-Based Nanomaterials Doped with CuNPs: An Inorganic–Organic Hybrid Recyclable Nanoenzyme as a Potent Peroxidase and Oxidase Mimic
AU - Meenakshi Goyal
AU - Anuj Kumar
AU - Pardeep Kumar
AU - Bhawna Uttam
PY - 2026
DA - 2026/10/16
JO - ACS Omega
DO - 10.1021/acsomega.6c07489
UR - https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c07489/5453036/Development-of-Supramolecular-Calix-4-arene-MCM
AB - An inorganic–organic hybrid nanomaterial, Cu@MCMCal, was synthesized by immobilizing p-tert-butyl calix[4]arene on to mesoporous silica followed by incorporation of CuNPs. The synthesized nanomaterial was characterized extensively using various analytical techniques such as FT-IR, UV–Vis spectroscopy, TEM, PXRD, XPS, Cu LMM Auger, BET, and TGA analyses. The hybrid nanomaterial was evaluated for its catalytic performance toward peroxidase-like activity using 3,3′,5,5′-tetramethylbenzidine (TMB) and o-phenylenediamine (OPD) as a substrate at room temperature at pH 4.5 in acetate buffer and pH 7.4 in PBS buffer, respectively. Enzyme kinetics was performed for both the substrates using the Michaelis–Menten steady-state kinetics and the Lineweaver–Burk plot to determine the catalytic affinity. Based on the kinetic measurements, the values of the Km for TMB and OPD were found to be 0.661 ± 0.143 and 0.606 ± 0.061 mM, respectively. The hybrid nanoenzyme also exhibits the oxidase-like activity using dopamine as a substrate at pH 7.4 in PBS buffer. The Cu@MCMCal nanomaterial acted as a nanoenzyme, efficient for catalysis toward oxidation reactions with better cost effectiveness and improved stability and recyclability as compared to biological enzymes. Therefore, a promising, stable, efficient, and robust nanoenzyme has been development for oxidation reactions.
SN - 2470-1343
ER -