Coordinated regulation of diverse F-actin organizations orchestrates F-actin pulses in the actomyosin network
Abstract
oogenesis, we report that basal pulsatile actomyosin networks consist of F-actin bundle and meshwork architectures, implicating the existence of different F-actin microstructures and corresponding nucleators. Here, Rac1 and the Scar/WAVE-Arp2/3 complex (branched F-actin nucleator), exhibiting constant levels, are necessary to support F-actin pulsation, whereas pulses of Dia (unbranched F-actin nucleator) trigger F-actin pulsation. The pulsatile F-actin networks recruit the F-actin turnover regulator cofilin, thus generating cofilin pulses. Cofilin at relatively low concentration can cooperate with F-actin nucleators to amplify F-actin and enhance its pulsation, while cofilin at relatively high concentration might trigger the F-actin disassembly to attenuate its pulsation. These two different effects of cofilin on F-actin pulsation were confirmed by Zdk-cofilin optogenetics. Rac1 signaling, Dia, and cofilin are also crucial for F-actin pulses in border cell migration. Our findings thus reveal a coordinated regulation of F-actin pulses conferring actomyosin contractility in morphogenesis and cell migration.
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BibTeX
@article{li2026coordinated,
title = {{Coordinated regulation of diverse F-actin organizations orchestrates F-actin pulses in the actomyosin network}},
author = {Hao Li and Bing Liu and Jiaying Liu and Thomas Mangeat and Karine Belguise and Shi-Lei Xue and Xiaobo Wang},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
year = {2026},
month = sep,
doi = {10.1073/pnas.2530056123},
url = {https://hal.science/hal-05852845v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Coordinated regulation of diverse F-actin organizations orchestrates F-actin pulses in the actomyosin network
AU - Hao Li
AU - Bing Liu
AU - Jiaying Liu
AU - Thomas Mangeat
AU - Karine Belguise
AU - Shi-Lei Xue
AU - Xiaobo Wang
PY - 2026
DA - 2026/09/08
JO - Proceedings of the National Academy of Sciences of the United States of America
DO - 10.1073/pnas.2530056123
UR - https://hal.science/hal-05852845v1
AB - oogenesis, we report that basal pulsatile actomyosin networks consist of F-actin bundle and meshwork architectures, implicating the existence of different F-actin microstructures and corresponding nucleators. Here, Rac1 and the Scar/WAVE-Arp2/3 complex (branched F-actin nucleator), exhibiting constant levels, are necessary to support F-actin pulsation, whereas pulses of Dia (unbranched F-actin nucleator) trigger F-actin pulsation. The pulsatile F-actin networks recruit the F-actin turnover regulator cofilin, thus generating cofilin pulses. Cofilin at relatively low concentration can cooperate with F-actin nucleators to amplify F-actin and enhance its pulsation, while cofilin at relatively high concentration might trigger the F-actin disassembly to attenuate its pulsation. These two different effects of cofilin on F-actin pulsation were confirmed by Zdk-cofilin optogenetics. Rac1 signaling, Dia, and cofilin are also crucial for F-actin pulses in border cell migration. Our findings thus reveal a coordinated regulation of F-actin pulses conferring actomyosin contractility in morphogenesis and cell migration.
SN - 0027-8424
SN - 1091-6490
ER -