Skip to content
DraconiticResearch
Search Sources API
203,948 studies31 sourcesUpdated 31 min ago
Peer-reviewedCC BY 4.0The Febs Journal

A guide to laser microdissection‐based proteomics

  • Cheng Shen1,2
  • Kael Schoffer1,2
  • Charlotte Williams1,2
  • Maria C. Tanzer1,2
  1. 1Walter and Eliza Hall Institute of Medical Research Parkville Australia
  2. 2University of Melbourne Parkville Australia
Read full textDOICite

Abstract

Laser microdissection‐based proteomics enables spatially resolved profiling of the proteome within intact tissue. Recent advances in histology, image analysis, laser microdissection and mass spectrometry have markedly increased the capability of this technology and driven the development of a range of powerful workflows. These now extend from regional tissue proteomics to emerging single‐cell applications, with subcellular proteome mapping on the horizon. In this review, we examine the current state of laser microdissection (LMD)‐based proteomics, outline what these approaches can offer, describe the standard workflow, and discuss which applications and instrumentation are best suited to address specific biological questions. We also highlight the strengths and limitations of current workflows and direct readers to key publications that describe and exemplify their successful implementation.

Related

  1. Identifying Differentially Expressed Proteins via Plasma Exosomal Proteomics
    Li-Li Zhang, Guo-Wei He
    Bio-protocolPeer-reviewedCC BY-NCFull textMolecular biology & gene regulationEurope PMC
  2. Integrated Spatial Metabolomics and Proteomics from the Same Tissue Section Using a Conductive ITO-PET Slide
    h. wang, Y. Li
    bioRxivPreprintAll rights reservedFull textMolecular biology & gene regulationbioRxiv
  3. Bioorthogonal Chemistry in Neurobiology: From Imaging to Proteomics
    Neil Taylor, João de Sousa‐Lourenço, Ivana Nikić‐Spiegel
    Chembiochem : a European journal of chemical biologyPeer-reviewedCC BYFull textMolecular biology & gene regulationEurope PMC
  4. Inhibitory Mechanism of Bee Venom Against A431 Cutaneous Squamous Cell Carcinoma Cells Based on Label-Free Proteomics and Untargeted Metabolomics
    International journal of molecular sciencesPeer-reviewedCC BYFull textGeneral biochemistryEurope PMC
  5. Nutritional modulation of microbiome proteomics in insulin resistance
    Beraat Dener, Sueda İzem Gülal, Miraç Bulut +3
    Frontiers in NutritionPeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationPMC

Cite

BibTeXRIS
BibTeX
@article{shen2026guide,
  title = {{A guide to laser microdissection‐based proteomics}},
  author = {Cheng Shen and Kael Schoffer and Charlotte Williams and Maria C. Tanzer},
  journal = {The Febs Journal},
  year = {2026},
  month = jul,
  doi = {10.1111/febs.70653},
  url = {https://doi.org/10.1111/febs.70653},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - A guide to laser microdissection‐based proteomics
AU  - Cheng Shen
AU  - Kael Schoffer
AU  - Charlotte Williams
AU  - Maria C. Tanzer
PY  - 2026
DA  - 2026/07/10
JO  - The Febs Journal
DO  - 10.1111/febs.70653
UR  - https://doi.org/10.1111/febs.70653
AB  - Laser microdissection‐based proteomics enables spatially resolved profiling of the proteome within intact tissue. Recent advances in histology, image analysis, laser microdissection and mass spectrometry have markedly increased the capability of this technology and driven the development of a range of powerful workflows. These now extend from regional tissue proteomics to emerging single‐cell applications, with subcellular proteome mapping on the horizon. In this review, we examine the current state of laser microdissection (LMD)‐based proteomics, outline what these approaches can offer, describe the standard workflow, and discuss which applications and instrumentation are best suited to address specific biological questions. We also highlight the strengths and limitations of current workflows and direct readers to key publications that describe and exemplify their successful implementation.
SN  - 1742-464X
SN  - 1742-4658
ER  -