Deer antler tip cell culture supernatant restores endothelial barrier function and angiotensin converting enzyme 2/endothelial nitric oxide synthase nitric oxide signaling impaired by Porphyromonas gingivalis lipopolysaccharide.
5 affiliations
- 1School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
- 2Department of Dentistry, Taipei Medical University-Shuang Ho Hospital, New Taipei City, Taiwan.
- 3Promax Medtech Group Inc., Taipei, Taiwan.
- 4Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Taipei Medical University, Taipei, Taiwan.
- 5Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan.
Abstract
Background/purpose
Periodontal inflammation contributes to endothelial dysfunction, but effective biologically active strategies for vascular protection remain limited. This study investigated whether deer antler tip cell culture supernatant could rescue Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) induced endothelial injury and restore endothelial homeostasis.Materials and methods
Velvet antler tissues from the tip, shaft, and burr regions were examined histologically and used for primary cell isolation. Human umbilical vein endothelial cells were exposed to Pg-LPS or indirectly co-cultured with LPS-stimulated THP-1-derived macrophages. Deer antler tip cell culture supernatant was subsequently applied to these endothelial inflammatory models, and its protective effects were assessed by quantitative functional assays, Western blotting, and qPCR. Public venous thromboembolism transcriptomic datasets were analyzed to evaluate the translational relevance of the endothelial injury and repair signatures.Results
The antler tip showed a vascular-rich regenerative niche with stem/progenitor-associated features. In HUVECs, Pg-LPS induced inflammatory activation, oxidative stress, and endothelial dysfunction, whereas deer antler tip cell culture supernatant attenuated these changes. It also suppressed macrophage-mediated endothelial injury and reduced Pg-LPS induced inflammatory activation in THP-1-derived macrophages. In an optimized endothelial injury model, deer antler tip cell culture supernatant dose-dependently restored endothelial homeostasis- and mechanosensitive-associated gene expression. Public datasets further supported the translational relevance of these endothelial stress signatures.Conclusion
Deer antler tip cell culture supernatant protects against Pg-LPS associated endothelial inflammatory injury and may serve as a promising biologically active strategy for vascular protection.
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BibTeX
@article{hsieh2026deer,
title = {{Deer antler tip cell culture supernatant restores endothelial barrier function and angiotensin converting enzyme 2/endothelial nitric oxide synthase nitric oxide signaling impaired by Porphyromonas gingivalis lipopolysaccharide.}},
author = {Ming-Shou Hsieh and Ya-Ting Yang and Chin-Sheng Huang and Ting-Yi Renn and Chuen-Kai Wang and Chia-Chen Hsu and Wei-Jen Chang and Yu-Sheng Chang},
journal = {Journal of dental sciences},
year = {2026},
month = oct,
doi = {10.1016/j.jds.2025.07.3521},
url = {https://europepmc.org/article/MED/42923693},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Deer antler tip cell culture supernatant restores endothelial barrier function and angiotensin converting enzyme 2/endothelial nitric oxide synthase nitric oxide signaling impaired by Porphyromonas gingivalis lipopolysaccharide.
AU - Ming-Shou Hsieh
AU - Ya-Ting Yang
AU - Chin-Sheng Huang
AU - Ting-Yi Renn
AU - Chuen-Kai Wang
AU - Chia-Chen Hsu
AU - Wei-Jen Chang
AU - Yu-Sheng Chang
PY - 2026
DA - 2026/10/01
JO - Journal of dental sciences
DO - 10.1016/j.jds.2025.07.3521
UR - https://europepmc.org/article/MED/42923693
AB - Background/purpose Periodontal inflammation contributes to endothelial dysfunction, but effective biologically active strategies for vascular protection remain limited. This study investigated whether deer antler tip cell culture supernatant could rescue Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) induced endothelial injury and restore endothelial homeostasis.Materials and methods Velvet antler tissues from the tip, shaft, and burr regions were examined histologically and used for primary cell isolation. Human umbilical vein endothelial cells were exposed to Pg-LPS or indirectly co-cultured with LPS-stimulated THP-1-derived macrophages. Deer antler tip cell culture supernatant was subsequently applied to these endothelial inflammatory models, and its protective effects were assessed by quantitative functional assays, Western blotting, and qPCR. Public venous thromboembolism transcriptomic datasets were analyzed to evaluate the translational relevance of the endothelial injury and repair signatures.Results The antler tip showed a vascular-rich regenerative niche with stem/progenitor-associated features. In HUVECs, Pg-LPS induced inflammatory activation, oxidative stress, and endothelial dysfunction, whereas deer antler tip cell culture supernatant attenuated these changes. It also suppressed macrophage-mediated endothelial injury and reduced Pg-LPS induced inflammatory activation in THP-1-derived macrophages. In an optimized endothelial injury model, deer antler tip cell culture supernatant dose-dependently restored endothelial homeostasis- and mechanosensitive-associated gene expression. Public datasets further supported the translational relevance of these endothelial stress signatures.Conclusion Deer antler tip cell culture supernatant protects against Pg-LPS associated endothelial inflammatory injury and may serve as a promising biologically active strategy for vascular protection.
SN - 1991-7902
SN - 2213-8862
ER -