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Peer-reviewedCC BY-NC-ND 4.0STAR Protocols

Protocol for studying membrane protein dynamics and associated synaptic vesicle recruitment on native membrane sheets

  • Akshay Kapadia1
  • Anne-Sophie Hafner1,2
  1. 1Donders Institute for Brain, Cognition and Behavior, Radboud University, Nijmegen, the Netherlands
  2. 2Interdisciplinary Institute for Neuroscience (IINS), Université de Bordeaux, UMR 5297 CNRS, 33076 Bordeaux, France
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Abstract

Plasma membrane sheets provide direct access to the cytosolic face of the cellular membrane while retaining membrane-associated proteins and lipids. Here, we present a protocol for generating and validating sonication-derived membrane sheets from cultured cells, primary neurons, and isolated synaptosomes. We describe procedures for real-time and fixed imaging of membrane protein organization, interactions, associated synaptic vesicle recruitment, and quantitative analysis. This platform is readily adaptable to investigate diverse membrane proteins, lipid-dependent mechanisms, and vesicle tethering events across cellular systems.

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Cite

BibTeXRIS
BibTeX
@article{kapadia2026protocol,
  title = {{Protocol for studying membrane protein dynamics and associated synaptic vesicle recruitment on native membrane sheets}},
  author = {Akshay Kapadia and Anne-Sophie Hafner},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104923},
  url = {https://doi.org/10.1016/j.xpro.2026.104923},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - Protocol for studying membrane protein dynamics and associated synaptic vesicle recruitment on native membrane sheets
AU  - Akshay Kapadia
AU  - Anne-Sophie Hafner
PY  - 2026
DA  - 2026/10/23
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104923
UR  - https://doi.org/10.1016/j.xpro.2026.104923
AB  - Plasma membrane sheets provide direct access to the cytosolic face of the cellular membrane while retaining membrane-associated proteins and lipids. Here, we present a protocol for generating and validating sonication-derived membrane sheets from cultured cells, primary neurons, and isolated synaptosomes. We describe procedures for real-time and fixed imaging of membrane protein organization, interactions, associated synaptic vesicle recruitment, and quantitative analysis. This platform is readily adaptable to investigate diverse membrane proteins, lipid-dependent mechanisms, and vesicle tethering events across cellular systems.
SN  - 2666-1667
ER  -