Co-occurrence of antimicrobial resistance and reduced disinfectant susceptibility in Campylobacter spp. along the chicken supply chain: A farm-to-fork public health concern
- 1Jiangsu Institute of Poultry Science, Yangzhou 225125, China
Abstract
Campylobacter spp. are major foodborne pathogens, yet integrated farm-to-fork studies addressing contamination dynamics, antimicrobial resistance, disinfectant susceptibility, lineage dissemination, and genome structure remain limited. In this study, a batch of yellow-feathered broilers in China was tracked from farm to slaughterhouse and retail to investigate Campylobacter along the chicken supply chain. Among 290 samples, 95 Campylobacter isolates were identified, including 39 Campylobacter jejuni (C. jejuni) and 56 Campylobacter coli (C. coli), yielding an overall isolation rate of 32.76%. The slaughterhouse stage showed the highest isolation rate (47.41%), indicating that processing was the major contamination amplification point, whereas 14.00% of retail samples remained positive. Antimicrobial susceptibility testing revealed a substantial resistance burden, particularly to ciprofloxacin, ceftiofur, trimethoprim-sulfamethoxazole, kanamycin, and tilmicosin. Disinfectant susceptibility testing showed that cetrimonium bromide minimum inhibitory concentrations (MICs) were generally higher than those of benzalkonium chloride, and C. coli tended to occupy higher MIC categories than C. jejuni. Integrated phenotype-genotype analysis showed clear associations between major resistance phenotypes and determinants, including T86I, tet(O), erm(B), 23S rRNA 2075 A > G, and blaOXA-61. Multilocus sequence typing (MLST) identified ST829 as a dissemination-associated high-risk lineage persisting from farm to retail. By integrating farm-to-fork surveillance with resistance and genomic analyses, this study highlights the food safety significance of poultry-associated Campylobacter and provides a valuable basis for risk-oriented monitoring and targeted intervention in the chicken supply chain.
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BibTeX
@article{zhou2026cooccurrence,
title = {{Co-occurrence of antimicrobial resistance and reduced disinfectant susceptibility in Campylobacter spp. along the chicken supply chain: A farm-to-fork public health concern}},
author = {Qian Zhou and Mengjun Tang and Xiaoyan Zhang and Xiujun Tang and Xingxing Yang and Yushi Gao},
journal = {One health (Amsterdam, Netherlands)},
year = {2026},
month = oct,
doi = {10.1016/j.onehlt.2026.101635},
url = {https://europepmc.org/article/PMC/PMC13722060},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - Co-occurrence of antimicrobial resistance and reduced disinfectant susceptibility in Campylobacter spp. along the chicken supply chain: A farm-to-fork public health concern
AU - Qian Zhou
AU - Mengjun Tang
AU - Xiaoyan Zhang
AU - Xiujun Tang
AU - Xingxing Yang
AU - Yushi Gao
PY - 2026
DA - 2026/10/24
JO - One health (Amsterdam, Netherlands)
DO - 10.1016/j.onehlt.2026.101635
UR - https://europepmc.org/article/PMC/PMC13722060
AB - Campylobacter spp. are major foodborne pathogens, yet integrated farm-to-fork studies addressing contamination dynamics, antimicrobial resistance, disinfectant susceptibility, lineage dissemination, and genome structure remain limited. In this study, a batch of yellow-feathered broilers in China was tracked from farm to slaughterhouse and retail to investigate Campylobacter along the chicken supply chain. Among 290 samples, 95 Campylobacter isolates were identified, including 39 Campylobacter jejuni (C. jejuni) and 56 Campylobacter coli (C. coli), yielding an overall isolation rate of 32.76%. The slaughterhouse stage showed the highest isolation rate (47.41%), indicating that processing was the major contamination amplification point, whereas 14.00% of retail samples remained positive. Antimicrobial susceptibility testing revealed a substantial resistance burden, particularly to ciprofloxacin, ceftiofur, trimethoprim-sulfamethoxazole, kanamycin, and tilmicosin. Disinfectant susceptibility testing showed that cetrimonium bromide minimum inhibitory concentrations (MICs) were generally higher than those of benzalkonium chloride, and C. coli tended to occupy higher MIC categories than C. jejuni. Integrated phenotype-genotype analysis showed clear associations between major resistance phenotypes and determinants, including T86I, tet(O), erm(B), 23S rRNA 2075 A > G, and blaOXA-61. Multilocus sequence typing (MLST) identified ST829 as a dissemination-associated high-risk lineage persisting from farm to retail. By integrating farm-to-fork surveillance with resistance and genomic analyses, this study highlights the food safety significance of poultry-associated Campylobacter and provides a valuable basis for risk-oriented monitoring and targeted intervention in the chicken supply chain.
SN - 2352-7714
ER -