Carbon Monoxide Generation, Heme Turnover, and Lipid Peroxidation During Heme-Mediated LDL Oxidation: Association of HO-1, Hemopexin, and A1M with LDL
6 affiliations
- 1Department of Internal Medicine, Division of Nephrology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary
- 2HUN-REN–UD Vascular Biology and Myocardium Pathophysiology Research Group, Hungarian Academy of Sciences, University of Debrecen, H-4032 Debrecen, Hungary
- 3Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary
- 4Department of Pharmacodynamics, Faculty of Pharmacy, University of Debrecen, H-4032 Debrecen, Hungary
- 5Department of Clinical Sciences Lund, Pediatrics, Lund University, 221 84 Lund, Sweden
- 6Department of Pediatrics, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary
Abstract
Heme promotes oxidative modification of low-density lipoprotein (LDL), a process implicated in atherosclerosis. However, the fate of heme during LDL oxidation and the mechanisms governing its degradation remain poorly understood. We investigated heme catabolism during heme/H2O2-induced LDL oxidation and identified LDL-associated proteins potentially involved in this process. Lipid peroxidation, heme Soret absorbance, and carbon monoxide (CO) production were assessed. Heme induced lipid peroxidation, loss of Soret signal, and CO generation, whereas CuSO4-mediated oxidation induced lipid peroxidation without detectable CO, supporting the association of CO production with heme-dependent oxidation. Targeted and data-independent proteomics and immunological analyses identified heme oxygenase-1 (HO-1), hemopexin (HPX), and alpha-1-microglobulin (A1M) in LDL. HO-1 inhibition reduced CO production and delayed early heme loss and lipid peroxidation, although these effects diminished over time. DTPA similarly delayed early heme loss and lipid peroxidation and reduced CO production, whereas BHT strongly suppressed lipid peroxidation, preserved the heme Soret signal, and reduced CO production throughout oxidation. HPX attenuated heme-induced LDL oxidation and CO generation. These findings indicate that HO-1-associated iron release contributes to early phase heme-mediated LDL oxidation along with CO generation, and heme-associated iron without liberation also contributes the oxidative modification of LDL.
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BibTeX
@article{petho2026carbon,
title = {{Carbon Monoxide Generation, Heme Turnover, and Lipid Peroxidation During Heme-Mediated LDL Oxidation: Association of HO-1, Hemopexin, and A1M with LDL}},
author = {Dávid Pethő and Gergő Kalló and Éva Csősz and István Bak and Magnus Gram and József Balla and György Balla and Tamás Gáll},
journal = {Biomolecules},
year = {2026},
month = oct,
doi = {10.3390/biom16101500},
url = {https://www.mdpi.com/2218-273X/16/10/1500},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Carbon Monoxide Generation, Heme Turnover, and Lipid Peroxidation During Heme-Mediated LDL Oxidation: Association of HO-1, Hemopexin, and A1M with LDL
AU - Dávid Pethő
AU - Gergő Kalló
AU - Éva Csősz
AU - István Bak
AU - Magnus Gram
AU - József Balla
AU - György Balla
AU - Tamás Gáll
PY - 2026
DA - 2026/10/01
JO - Biomolecules
DO - 10.3390/biom16101500
UR - https://www.mdpi.com/2218-273X/16/10/1500
AB - Heme promotes oxidative modification of low-density lipoprotein (LDL), a process implicated in atherosclerosis. However, the fate of heme during LDL oxidation and the mechanisms governing its degradation remain poorly understood. We investigated heme catabolism during heme/H2O2-induced LDL oxidation and identified LDL-associated proteins potentially involved in this process. Lipid peroxidation, heme Soret absorbance, and carbon monoxide (CO) production were assessed. Heme induced lipid peroxidation, loss of Soret signal, and CO generation, whereas CuSO4-mediated oxidation induced lipid peroxidation without detectable CO, supporting the association of CO production with heme-dependent oxidation. Targeted and data-independent proteomics and immunological analyses identified heme oxygenase-1 (HO-1), hemopexin (HPX), and alpha-1-microglobulin (A1M) in LDL. HO-1 inhibition reduced CO production and delayed early heme loss and lipid peroxidation, although these effects diminished over time. DTPA similarly delayed early heme loss and lipid peroxidation and reduced CO production, whereas BHT strongly suppressed lipid peroxidation, preserved the heme Soret signal, and reduced CO production throughout oxidation. HPX attenuated heme-induced LDL oxidation and CO generation. These findings indicate that HO-1-associated iron release contributes to early phase heme-mediated LDL oxidation along with CO generation, and heme-associated iron without liberation also contributes the oxidative modification of LDL.
SN - 2218-273X
ER -