Red Wine Lees Valorization for Phenolic Compounds Recovery and Characterization Using Resin Adsorption
- 1Department of Chemical Engineering, University of Patras, 26504 Patras, Greece
- 2Department of Sustainable Agriculture, University of Patras, 30100 Agrinio, Greece
- 3Foundation for Research and Technology—Hellas, Institute of Chemical Engineering Sciences, FORTH/ICE-HT, 26504 Patras, Greece
Abstract
Wine lees are a phenolic-rich winery by-product whose liquid fraction remains underexploited. This study investigated the direct recovery of phenolic compounds from the liquid fraction of red wine lees (LfWL) using the non-ionic Amberlite XAD-16 resin through batch and fixed-bed adsorption, followed by sequential desorption and LC-HRMS profiling. Batch adsorption experiments were conducted at resin dosages ranging from 20 to 120 g/L. The highest adsorption yield (89.9%) was achieved at 120 g/L, whereas the highest experimental adsorption capacity (123.5 mg SAE/g) was obtained at 20 g/L. Adsorption kinetics were better described by the pseudo-second-order model, whereas the non-linear Langmuir model provided the best equilibrium fit, with a model-estimated maximum adsorption capacity of 304.34 mg SAE/g. Phenolics adsorption was favored at the natural pH of the LfWL, while the thermodynamic analysis indicated an exothermic and thermodynamically favorable process. Two different fixed-bed columns were evaluated under continuous flow conditions. The configuration with larger dimensions (LFB-200) treated an undiluted LfWL, reaching an adsorption efficiency of 36.59% with a breakthrough profile extending beyond 1400 min. Sequential elution with water followed by a 50% (v/v) ethanol/water mixture resulted in desorption yields of 88.96% and 79.54% for the small and large columns, respectively. LC-HRMS enabled the putative annotation of 20 phenolic compounds and revealed compound and class-dependent elution profiles. These results support resin adsorption as a promising route for direct phenolic recovery from liquid wine lees fractions.
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BibTeX
@article{vasilopoulos2026wine,
title = {{Red Wine Lees Valorization for Phenolic Compounds Recovery and Characterization Using Resin Adsorption}},
author = {Antonis Vasilopoulos and Varvara Sygouni and Christina Vasiliki Lazaratou and Athanasia G. Tekerlekopoulou and Dimitris V. Vayenas},
journal = {Molecules},
year = {2026},
month = nov,
doi = {10.3390/molecules31213929},
url = {https://www.mdpi.com/1420-3049/31/21/3929},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Red Wine Lees Valorization for Phenolic Compounds Recovery and Characterization Using Resin Adsorption
AU - Antonis Vasilopoulos
AU - Varvara Sygouni
AU - Christina Vasiliki Lazaratou
AU - Athanasia G. Tekerlekopoulou
AU - Dimitris V. Vayenas
PY - 2026
DA - 2026/11/01
JO - Molecules
DO - 10.3390/molecules31213929
UR - https://www.mdpi.com/1420-3049/31/21/3929
AB - Wine lees are a phenolic-rich winery by-product whose liquid fraction remains underexploited. This study investigated the direct recovery of phenolic compounds from the liquid fraction of red wine lees (LfWL) using the non-ionic Amberlite XAD-16 resin through batch and fixed-bed adsorption, followed by sequential desorption and LC-HRMS profiling. Batch adsorption experiments were conducted at resin dosages ranging from 20 to 120 g/L. The highest adsorption yield (89.9%) was achieved at 120 g/L, whereas the highest experimental adsorption capacity (123.5 mg SAE/g) was obtained at 20 g/L. Adsorption kinetics were better described by the pseudo-second-order model, whereas the non-linear Langmuir model provided the best equilibrium fit, with a model-estimated maximum adsorption capacity of 304.34 mg SAE/g. Phenolics adsorption was favored at the natural pH of the LfWL, while the thermodynamic analysis indicated an exothermic and thermodynamically favorable process. Two different fixed-bed columns were evaluated under continuous flow conditions. The configuration with larger dimensions (LFB-200) treated an undiluted LfWL, reaching an adsorption efficiency of 36.59% with a breakthrough profile extending beyond 1400 min. Sequential elution with water followed by a 50% (v/v) ethanol/water mixture resulted in desorption yields of 88.96% and 79.54% for the small and large columns, respectively. LC-HRMS enabled the putative annotation of 20 phenolic compounds and revealed compound and class-dependent elution profiles. These results support resin adsorption as a promising route for direct phenolic recovery from liquid wine lees fractions.
SN - 1420-3049
ER -