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Peer-reviewedCC BY 4.0Biomolecules

Cytochalasin B Alleviates LPS-Induced Mastitis and Modulates the Expression of PI3K/N-WASP/ARP2/3-Related Molecules

  • Weitao Dong1,2
  • An Wang1,2
  • Yaru Li1,2
  • Xingcai Qi2,3
  • Wenkai Bai1,2
  • Kejiang Liu1,2
  • Quanwei Zhang2,4
  • Xingxu Zhao1,2
  • Yong Zhang1,2
4 affiliations
  1. 1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
  2. 2Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China
  3. 3Lanzhou Modern Vacational College, Lanzhou 730030, China
  4. 4College of Life Sciences and Biotechnology, Gansu Agricultural University, Lanzhou 730030, China
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Abstract

Cytochalasin B (CB) inhibits actin polymerization and modulates cytoskeletal dynamics, but its role in mastitis remains unclear. This study investigated the protective effects of CB against mastitis and its association with PI3K/N-WASP/ARP2/3-related molecular alterations. Transcriptomic analysis of mammary tissues from healthy cows and cows with subclinical or clinical mastitis revealed extensive transcriptional remodeling during mastitis progression. Validation showed enhanced inflammatory responses accompanied by increased expression of PI3K, N-WASP, CLTC, ARP2, and ARP3 in mastitic tissues. In LPS-stimulated bovine mammary epithelial cells (MAC-T), CB reduced pro-inflammatory cytokine expression, increased IL-10 expression, and attenuated the abnormal elevation of PI3K/N-WASP/ARP2/3-related molecules. Consistently, in an LPS-induced mouse mastitis model, CB alleviated mammary tissue injury and inflammatory cell infiltration, improved inflammatory cytokine profiles, and reduced the expression of PI3K, N-WASP, CLTC, ARP2, and ARP3. Collectively, CB attenuates LPS-induced mammary inflammation and tissue injury, and these effects are associated with alterations in PI3K/N-WASP/ARP2/3-related cytoskeletal molecules. These findings provide new evidence linking cytoskeleton-related molecular alterations to mastitis inflammation and the anti-inflammatory effects of CB.

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Cite

BibTeXRIS
BibTeX
@article{dong2026cytochalasin,
  title = {{Cytochalasin B Alleviates LPS-Induced Mastitis and Modulates the Expression of PI3K/N-WASP/ARP2/3-Related Molecules}},
  author = {Weitao Dong and An Wang and Yaru Li and Xingcai Qi and Wenkai Bai and Kejiang Liu and Quanwei Zhang and Xingxu Zhao and Yong Zhang},
  journal = {Biomolecules},
  year = {2026},
  month = oct,
  doi = {10.3390/biom16101467},
  url = {https://www.mdpi.com/2218-273X/16/10/1467},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Cytochalasin B Alleviates LPS-Induced Mastitis and Modulates the Expression of PI3K/N-WASP/ARP2/3-Related Molecules
AU  - Weitao Dong
AU  - An Wang
AU  - Yaru Li
AU  - Xingcai Qi
AU  - Wenkai Bai
AU  - Kejiang Liu
AU  - Quanwei Zhang
AU  - Xingxu Zhao
AU  - Yong Zhang
PY  - 2026
DA  - 2026/10/01
JO  - Biomolecules
DO  - 10.3390/biom16101467
UR  - https://www.mdpi.com/2218-273X/16/10/1467
AB  - Cytochalasin B (CB) inhibits actin polymerization and modulates cytoskeletal dynamics, but its role in mastitis remains unclear. This study investigated the protective effects of CB against mastitis and its association with PI3K/N-WASP/ARP2/3-related molecular alterations. Transcriptomic analysis of mammary tissues from healthy cows and cows with subclinical or clinical mastitis revealed extensive transcriptional remodeling during mastitis progression. Validation showed enhanced inflammatory responses accompanied by increased expression of PI3K, N-WASP, CLTC, ARP2, and ARP3 in mastitic tissues. In LPS-stimulated bovine mammary epithelial cells (MAC-T), CB reduced pro-inflammatory cytokine expression, increased IL-10 expression, and attenuated the abnormal elevation of PI3K/N-WASP/ARP2/3-related molecules. Consistently, in an LPS-induced mouse mastitis model, CB alleviated mammary tissue injury and inflammatory cell infiltration, improved inflammatory cytokine profiles, and reduced the expression of PI3K, N-WASP, CLTC, ARP2, and ARP3. Collectively, CB attenuates LPS-induced mammary inflammation and tissue injury, and these effects are associated with alterations in PI3K/N-WASP/ARP2/3-related cytoskeletal molecules. These findings provide new evidence linking cytoskeleton-related molecular alterations to mastitis inflammation and the anti-inflammatory effects of CB.
SN  - 2218-273X
ER  -