Immunohistochemical expression of NF-κB signaling pathway components in the septum of type I intestinal atresia
5 affiliations
- 1Department of General Surgery, Capital Center for Children’s Health, Capital Medical University, Capital Institute of Pediatrics , ,
- 2Department of Pathology, Beijing Children’s Hospital Heilongjiang Hospital , ,
- 3Department of Science and Technology, Capital Center for Children's Health, Capital Medical University, Capital Institute of Pediatrics , ,
- 4Department of Neonatal Surgery, Capital Center for Children’s Health, Capital Medical University, Capital Institute of Pediatrics , ,
- 5Department of Biochemistry and Immunology, Capital Center for Children's Health, Capital Medical University, Capital Institute of Pediatrics , ,
Abstract
Background: The aim of the study was to characterize the expression of nuclear factor (NF)-κB signaling pathway molecules in the septal mucosa of type I intestinal atresia (IA) and to explore their potential association with impaired intestinal recanalization during embryonic development.
Methods: Septa and adjacent proximal intestinal wall tissue were obtained from eight patients with type I IA. Serial sections were prepared for immunohistochemistry staining of transforming growth factor-β-activated kinase 1 (TAK1), I kappa B kinase-alpha/beta (IKK-α/β), p65 NF-κB, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6). Mean optical density was quantified using Image-Pro Plus software, and differences between groups were statistically analyzed.
Results: TAK1, IKK-α/β, and p65 NF-κB showed weak cytoplasmic immunoreactivity in the glandular epithelial cells of the control intestinal mucosa. In contrast, markedly increased cytoplasmic staining for TAK1, IKK-α/β, and p65 NF-κB proteins was observed in the mucosal glands of septa. The expression of TNF-α and IL-6, typical downstream pro-inflammatory cytokines of the NF-κB signaling pathway, demonstrated markedly increased cytoplasmic staining. A semiquantitative analysis confirmed a significantly higher expression of TAK1, IKK-α/β, p65 NF-κB, TNF-α, and IL-6 in septa than in the normal intestinal wall.
Conclusion: The aberrant expression of the NF-κB signaling pathway and activation of the inflammatory response during embryonic intestinal recanalization may be associated with the formation and progression of septa.
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BibTeX
@article{liu2026immunohistochemical,
title = {{Immunohistochemical expression of NF-κB signaling pathway components in the septum of type I intestinal atresia}},
author = {Xuelai Liu and Huan Wang and Yuqiang Chen and Mao Ye and Xiaoyun Cui and Yandong Wei and Feng He},
journal = {Frontiers in pediatrics},
year = {2026},
month = oct,
doi = {10.3389/fped.2026.1978002},
url = {https://europepmc.org/article/PMC/PMC13724578},
note = {CC BY},
}
RIS
TY - JOUR
TI - Immunohistochemical expression of NF-κB signaling pathway components in the septum of type I intestinal atresia
AU - Xuelai Liu
AU - Huan Wang
AU - Yuqiang Chen
AU - Mao Ye
AU - Xiaoyun Cui
AU - Yandong Wei
AU - Feng He
PY - 2026
DA - 2026/10/19
JO - Frontiers in pediatrics
DO - 10.3389/fped.2026.1978002
UR - https://europepmc.org/article/PMC/PMC13724578
AB - Background: The aim of the study was to characterize the expression of nuclear factor (NF)-κB signaling pathway molecules in the septal mucosa of type I intestinal atresia (IA) and to explore their potential association with impaired intestinal recanalization during embryonic development. Methods: Septa and adjacent proximal intestinal wall tissue were obtained from eight patients with type I IA. Serial sections were prepared for immunohistochemistry staining of transforming growth factor-β-activated kinase 1 (TAK1), I kappa B kinase-alpha/beta (IKK-α/β), p65 NF-κB, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6). Mean optical density was quantified using Image-Pro Plus software, and differences between groups were statistically analyzed. Results: TAK1, IKK-α/β, and p65 NF-κB showed weak cytoplasmic immunoreactivity in the glandular epithelial cells of the control intestinal mucosa. In contrast, markedly increased cytoplasmic staining for TAK1, IKK-α/β, and p65 NF-κB proteins was observed in the mucosal glands of septa. The expression of TNF-α and IL-6, typical downstream pro-inflammatory cytokines of the NF-κB signaling pathway, demonstrated markedly increased cytoplasmic staining. A semiquantitative analysis confirmed a significantly higher expression of TAK1, IKK-α/β, p65 NF-κB, TNF-α, and IL-6 in septa than in the normal intestinal wall. Conclusion: The aberrant expression of the NF-κB signaling pathway and activation of the inflammatory response during embryonic intestinal recanalization may be associated with the formation and progression of septa.
SN - 2296-2360
ER -