Dietary Exposure to Purified Gliadins and Avenins: Effects on Intestinal and Immune Responses in BALB/c Mice
- 1Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic
Abstract
Dietary cereal prolamins differ in composition and gastrointestinal processing, but their shared and protein-source-specific effects on intestinal homeostasis remain incompletely defined. BALB/c mice were fed for five weeks with diets in which 70% of total protein was supplied by vital wheat gluten, purified gliadin, or purified avenin, alongside casein-fed controls. Intestinal morphology, antigen-specific plasma immunoglobulin G and A (IgG, IgA), mucosal gene expression, and small-intestinal proteomics were analysed. No overt histopathological injury or generalised inflammatory response was detected in any group. Gliadin and avenin both increased ileal villus length; avenin additionally elicited the broadest and most frequent prolamin-specific IgG response, associated with reduced intestinal interleukin-10 expression in responding animals. Proteomic profiling revealed a shared structural-metabolic signature alongside protein-specific changes in lipid handling, junctional regulation, and epithelial immune signalling. These findings indicate that wheat- and oat-derived prolamins differentially influence intestinal homeostasis and antigen recognition even at matched dietary doses, informing their evaluation as functional food ingredients.
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BibTeX
@misc{stastna2026dietary,
title = {{Dietary Exposure to Purified Gliadins and Avenins: Effects on Intestinal and Immune Responses in BALB/c Mice}},
author = {M. Stastna and A. Norek and P. Makovicky and E. Jeklova and P. Strakova and M. Hrunka and P. Jabandziev and L. Janda},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.08.757589},
url = {https://www.biorxiv.org/content/10.64898/2026.10.08.757589v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Dietary Exposure to Purified Gliadins and Avenins: Effects on Intestinal and Immune Responses in BALB/c Mice
AU - M. Stastna
AU - A. Norek
AU - P. Makovicky
AU - E. Jeklova
AU - P. Strakova
AU - M. Hrunka
AU - P. Jabandziev
AU - L. Janda
PY - 2026
DA - 2026/10/08
PB - bioRxiv
DO - 10.64898/2026.10.08.757589
UR - https://www.biorxiv.org/content/10.64898/2026.10.08.757589v1
AB - Dietary cereal prolamins differ in composition and gastrointestinal processing, but their shared and protein-source-specific effects on intestinal homeostasis remain incompletely defined. BALB/c mice were fed for five weeks with diets in which 70% of total protein was supplied by vital wheat gluten, purified gliadin, or purified avenin, alongside casein-fed controls. Intestinal morphology, antigen-specific plasma immunoglobulin G and A (IgG, IgA), mucosal gene expression, and small-intestinal proteomics were analysed. No overt histopathological injury or generalised inflammatory response was detected in any group. Gliadin and avenin both increased ileal villus length; avenin additionally elicited the broadest and most frequent prolamin-specific IgG response, associated with reduced intestinal interleukin-10 expression in responding animals. Proteomic profiling revealed a shared structural-metabolic signature alongside protein-specific changes in lipid handling, junctional regulation, and epithelial immune signalling. These findings indicate that wheat- and oat-derived prolamins differentially influence intestinal homeostasis and antigen recognition even at matched dietary doses, informing their evaluation as functional food ingredients.
ER -