RhodaMal Dyes: Novel Red Emissive Fluorophores as Probes for High Contrast Live‐Cell Imaging
- 1Institute of Chemistry and Biochemistry Freie Universität Berlin Berlin Germany
- 2SupraFAB Freie Universität Berlin Berlin Germany
- 3Institute For Biology/Genetics Freie Universität Berlin Berlin Germany
Abstract
Red and near‐infrared emitting dyes are of high interest in fluorescence microscopy. Herein, we report two new red emissive dyes, namely RhodaMal 620 and RhodaMal 638 by incorporation of malononitrile, a highly electron‐withdrawing and π‐extending moiety into the traditional rhodol framework. The dyes have their absorption and emission maxima at 620/638 and 664/708 nm in aqueous solution. By increasing the viscosity of the medium to mimic the microenvironment of crowded and viscous subcellular compartments, we showed a drastic 20‐fold increase in the fluorescence signal. Conjugating the dyes to triphenylphosphonium cation (TPP+) enabled efficient mitochondrial targeting. RhodaMal 620 was further used in live cell STED microscopy with a 775 nm depletion laser, enabling super resolution with raw full‐width at half maximum values down to 77 nm.
Related
- Imaging Contrast of Diamond Nanoparticle Structures on Different Supporting Films
- Electrically switchable liquid crystal lens for bright-field and spiral phase contrast imaging
- Recent Advances in Near-Infrared Fluorescent Probes Based on Organic Small Molecules for the Detection and Imaging of Heavy Metal Ions
- Aggregation-Induced Emission Probes for Migrasome Imaging and Visualization
- High resolution X-ray phase contrast imaging for non-destructive testing of composite materials (2022)
BibTeX
@article{mavroskoufis2026rhodamal,
title = {{RhodaMal Dyes: Novel Red Emissive Fluorophores as Probes for High Contrast Live‐Cell Imaging}},
author = {A. Mavroskoufis and P. Aßmann and L. M. Filla and I. Bilici and E. Quaas and M. Maglione and S. Sigrist and R. Haag and K. Licha},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
year = {2026},
month = jul,
doi = {10.1002/chem.71410},
url = {https://doi.org/10.1002/chem.71410},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - RhodaMal Dyes: Novel Red Emissive Fluorophores as Probes for High Contrast Live‐Cell Imaging
AU - A. Mavroskoufis
AU - P. Aßmann
AU - L. M. Filla
AU - I. Bilici
AU - E. Quaas
AU - M. Maglione
AU - S. Sigrist
AU - R. Haag
AU - K. Licha
PY - 2026
DA - 2026/07/18
JO - Chemistry (Weinheim an Der Bergstrasse, Germany)
DO - 10.1002/chem.71410
UR - https://doi.org/10.1002/chem.71410
AB - Red and near‐infrared emitting dyes are of high interest in fluorescence microscopy. Herein, we report two new red emissive dyes, namely RhodaMal 620 and RhodaMal 638 by incorporation of malononitrile, a highly electron‐withdrawing and π‐extending moiety into the traditional rhodol framework. The dyes have their absorption and emission maxima at 620/638 and 664/708 nm in aqueous solution. By increasing the viscosity of the medium to mimic the microenvironment of crowded and viscous subcellular compartments, we showed a drastic 20‐fold increase in the fluorescence signal. Conjugating the dyes to triphenylphosphonium cation (TPP+) enabled efficient mitochondrial targeting. RhodaMal 620 was further used in live cell STED microscopy with a 775 nm depletion laser, enabling super resolution with raw full‐width at half maximum values down to 77 nm.
SN - 0947-6539
SN - 1521-3765
ER -