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Peer-reviewedCC BY 4.0Folia Microbiologica

EDTA enhances antimicrobial activity of PR-39 and Protegrin-1 antimicrobial peptides against carbapenem-resistant Pseudomonas aeruginosa in serum

  • Lukáš Vacek1
  • Antonín Pavelka2,3
  • Břetislav Lipový4,5
  • Jana Brtníková5
  • Petra Straková2
  • Edita Jeklová2
  • Marko Šefranko1
  • Dominika Polaštík Kleknerová1
  • Frederik Volný1
  • Lubomír Janda2
  • Lucy Vojtová5
  • Filip Růžička1
5 affiliations
  1. 1Department of Microbiology, Faculty of Medicine, Masaryk University and St. Anne’s University Hospital, Pekařská 53, Brno, 602 00 Czech Republic
  2. 2Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Hudcova 296/70, Brno, 621 00 Czech Republic
  3. 3National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, Brno, 625 00 Czech Republic
  4. 4Department of Burns Medicine, Third Faculty of Medicine, Charles University, University Hospital Královské Vinohrady, Šrobárova 50, Prague, 100 34 Czech Republic
  5. 5Advanced Biomaterials Group, Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, Brno, 612 00 Czech Republic
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Abstract

Carbapenem-resistant Pseudomonas aeruginosa represents a frequent and clinically challenging pathogen responsible for both acute and chronic infections. Antimicrobial peptides are emerging as a promising class of novel therapeutic agents due to their broad-spectrum activity, rapid bactericidal activity, and low potential to induce antimicrobial resistance. The antimicrobial efficacy of these peptides can be further enhanced by EDTA. However, their activity is often reduced in the presence of serum, which contains multiple inhibitory components. In this study, EDTA fully restored the antimicrobial activity of PR-39 and Protegrin-1 in serum. Moreover, incorporation of chitosan increased the antimicrobial efficacy of the PR-39/Protegrin-1/EDTA formulation. These findings demonstrate that PR-39, Protegrin-1, EDTA, and chitosan can act synergistically, supporting the feasibility of integrating these components into a unified therapeutic platform.

Supplementary information: The online version contains supplementary material available at https://doi.org/10.1007/s12223-026-01521-2.

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Cite

BibTeXRIS
BibTeX
@article{vacek2026edta,
  title = {{EDTA enhances antimicrobial activity of PR-39 and Protegrin-1 antimicrobial peptides against carbapenem-resistant Pseudomonas aeruginosa in serum}},
  author = {Lukáš Vacek and Antonín Pavelka and Břetislav Lipový and Jana Brtníková and Petra Straková and Edita Jeklová and Marko Šefranko and Dominika Polaštík Kleknerová and Frederik Volný and Lubomír Janda and Lucy Vojtová and Filip Růžička},
  journal = {Folia Microbiologica},
  year = {2026},
  month = may,
  doi = {10.1007/s12223-026-01521-2},
  url = {https://doi.org/10.1007/s12223-026-01521-2},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - EDTA enhances antimicrobial activity of PR-39 and Protegrin-1 antimicrobial peptides against carbapenem-resistant Pseudomonas aeruginosa in serum
AU  - Lukáš Vacek
AU  - Antonín Pavelka
AU  - Břetislav Lipový
AU  - Jana Brtníková
AU  - Petra Straková
AU  - Edita Jeklová
AU  - Marko Šefranko
AU  - Dominika Polaštík Kleknerová
AU  - Frederik Volný
AU  - Lubomír Janda
AU  - Lucy Vojtová
AU  - Filip Růžička
PY  - 2026
DA  - 2026/05/28
JO  - Folia Microbiologica
DO  - 10.1007/s12223-026-01521-2
UR  - https://doi.org/10.1007/s12223-026-01521-2
AB  - Carbapenem-resistant Pseudomonas aeruginosa represents a frequent and clinically challenging pathogen responsible for both acute and chronic infections. Antimicrobial peptides are emerging as a promising class of novel therapeutic agents due to their broad-spectrum activity, rapid bactericidal activity, and low potential to induce antimicrobial resistance. The antimicrobial efficacy of these peptides can be further enhanced by EDTA. However, their activity is often reduced in the presence of serum, which contains multiple inhibitory components. In this study, EDTA fully restored the antimicrobial activity of PR-39 and Protegrin-1 in serum. Moreover, incorporation of chitosan increased the antimicrobial efficacy of the PR-39/Protegrin-1/EDTA formulation. These findings demonstrate that PR-39, Protegrin-1, EDTA, and chitosan can act synergistically, supporting the feasibility of integrating these components into a unified therapeutic platform.  Supplementary information: The online version contains supplementary material available at https://doi.org/10.1007/s12223-026-01521-2.
SN  - 0015-5632
SN  - 1874-9356
ER  -