Conserved Obg-type GTPases Sustain Protein Synthesis across Domains of Life
- 1University of Illinois at Urbana-Champaign
Abstract
Translation is regulated both temporally and spatially to ensure that proteins are synthesized at the correct time and delivered to the proper subcellular location. Temporary slowing or halting of ribosomal movement along an mRNA, a physiological pausing, allows cells to coordinate protein production with internal cues and external signals. However, how cells distinguish such constructive pauses from harmful pathological stalls remains a fundamental unresolved question in molecular biology. Here, we report that a ribosome-binding GTPase (Rbg in yeast, and so-called Developmentally Regulated GTP-binding (Drg) in human) promotes protein biosynthesis in halted ribosomes in an evolutionarily conserved manner. We show that the essential bacterial Obg GTPase rescues cell growth and translation in Saccharomyces cerevisiae and human cells lacking their endogenous Rbg/Drg proteins in a GTPase-dependent manner. Furthermore, Obg enhances puromycin incorporation into stalled ribosomes in a manner requiring GTP hydrolysis. We reason that Rbg/Drg proteins act to prevent premature engagement of ribosome-associated quality-control or stress-response pathways. Under physiological conditions, proteomic analyses of Rbg1-bound ribosomes in yeast reveal that Rbg GTPases associate with ribosomes translating proteins essential for diverse functions, including those of the nucleus, ER, Golgi, mitochondria, and protein transport pathways. Finally, loss of even one of the two eukaryotic Rbg/Drg paralogs impedes cell-cycle progression, disrupts DNA replication, and compromises mitochondrial function. Together, these findings suggest that Rbg/Drg proteins enable cells across different domains of life to protect physiological ribosomal pauses, revealing a previously unrecognized layer of regulation in gene expression.
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BibTeX
@misc{saha2026conserved,
title = {{Conserved Obg-type GTPases Sustain Protein Synthesis across Domains of Life}},
author = {S. Saha and C. W. Hawk and X. Chen and A. Bhattacharya and S. Nizami and M. Pires-Alves and G. J. Olsen and H. Jin},
howpublished = {bioRxiv},
year = {2026},
month = may,
doi = {10.64898/2026.05.13.724932},
url = {https://www.biorxiv.org/content/10.64898/2026.05.13.724932v2},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Conserved Obg-type GTPases Sustain Protein Synthesis across Domains of Life
AU - S. Saha
AU - C. W. Hawk
AU - X. Chen
AU - A. Bhattacharya
AU - S. Nizami
AU - M. Pires-Alves
AU - G. J. Olsen
AU - H. Jin
PY - 2026
DA - 2026/05/13
PB - bioRxiv
DO - 10.64898/2026.05.13.724932
UR - https://www.biorxiv.org/content/10.64898/2026.05.13.724932v2
AB - Translation is regulated both temporally and spatially to ensure that proteins are synthesized at the correct time and delivered to the proper subcellular location. Temporary slowing or halting of ribosomal movement along an mRNA, a physiological pausing, allows cells to coordinate protein production with internal cues and external signals. However, how cells distinguish such constructive pauses from harmful pathological stalls remains a fundamental unresolved question in molecular biology. Here, we report that a ribosome-binding GTPase (Rbg in yeast, and so-called Developmentally Regulated GTP-binding (Drg) in human) promotes protein biosynthesis in halted ribosomes in an evolutionarily conserved manner. We show that the essential bacterial Obg GTPase rescues cell growth and translation in Saccharomyces cerevisiae and human cells lacking their endogenous Rbg/Drg proteins in a GTPase-dependent manner. Furthermore, Obg enhances puromycin incorporation into stalled ribosomes in a manner requiring GTP hydrolysis. We reason that Rbg/Drg proteins act to prevent premature engagement of ribosome-associated quality-control or stress-response pathways. Under physiological conditions, proteomic analyses of Rbg1-bound ribosomes in yeast reveal that Rbg GTPases associate with ribosomes translating proteins essential for diverse functions, including those of the nucleus, ER, Golgi, mitochondria, and protein transport pathways. Finally, loss of even one of the two eukaryotic Rbg/Drg paralogs impedes cell-cycle progression, disrupts DNA replication, and compromises mitochondrial function. Together, these findings suggest that Rbg/Drg proteins enable cells across different domains of life to protect physiological ribosomal pauses, revealing a previously unrecognized layer of regulation in gene expression.
ER -