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Peer-reviewedCC BYSTAR Protocols

Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling

  • Andrew Jones
  • Helen Flynn
  • Frank Uhlmann
  • Ambrosius Snijders
  • Sandra Touati
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Abstract

Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phosphatase mutant background to characterize phosphoproteome dynamics. Here, we provide a detailed protocol for biological and mass spectrometry sample preparation and analysis. For complete details on the use and execution of this protocol, please refer to Touati et al. (2019).

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Cite

BibTeXRIS
BibTeX
@article{jones2026assessing,
  title = {{Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling}},
  author = {Andrew Jones and Helen Flynn and Frank Uhlmann and Ambrosius Snijders and Sandra Touati},
  journal = {STAR Protocols},
  year = {2026},
  month = sep,
  doi = {10.1016/j.xpro.2020.100022},
  url = {https://hal.science/hal-05750285v1},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling
AU  - Andrew Jones
AU  - Helen Flynn
AU  - Frank Uhlmann
AU  - Ambrosius Snijders
AU  - Sandra Touati
PY  - 2026
DA  - 2026/09/15
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2020.100022
UR  - https://hal.science/hal-05750285v1
AB  - Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phosphatase mutant background to characterize phosphoproteome dynamics. Here, we provide a detailed protocol for biological and mass spectrometry sample preparation and analysis. For complete details on the use and execution of this protocol, please refer to Touati et al. (2019).
SN  - 2666-1667
ER  -