Extended normothermic machine perfusion preserves viability and tissue integrity of ex vivo human intestine segments
- 1Mayo Clinic
Abstract
Human intestinal diseases, including inflammatory bowel disease (IBD), are difficult to model because existing animal and in vitro systems do not capture the integrated vascular, immune, and metabolic complexity of the intestine. Here, we establish a custom normothermic machine perfusion platform that maintains surgically resected human intestinal tissue for up to 72 hours under near-physiological conditions. Discarded specimens from patients with nonmalignant intestinal diseases, including IBD and diverticulitis, were continuously perfused at 37{degrees}C with real-time hemodynamic and metabolic monitoring. Perfusion preserved tissue viability, mucosal architecture, epithelial integrity, oxygen consumption, metabolic activity, and vascular patency. Daily perfusate sampling enabled longitudinal profiling and experimental modulation of inflammatory mediators, revealing patient-specific immune states. This long-duration platform bridges reductionist in vitro models and clinical disease, enabling mechanistic studies of intestinal inflammation and future testing of personalized therapies in intact patient-derived tissue.
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BibTeX
@misc{urbina2026extended,
title = {{Extended normothermic machine perfusion preserves viability and tissue integrity of ex vivo human intestine segments}},
author = {P. A. M. Urbina and S. R. Choi and D. C. Cheung and L. K. Egbert and M. I. Garcia-Neuer and S. Yu and N. S. Rehman and D. G. Hanks and T.-A. Le and H. H. Alabay and K. E. McHugh and F. H. Hamdan and N. Naik and W. A. Faubion and J. T. Brady and H. Ceylan and S. Bose},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.17.752436},
url = {https://www.biorxiv.org/content/10.64898/2026.09.17.752436v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Extended normothermic machine perfusion preserves viability and tissue integrity of ex vivo human intestine segments
AU - P. A. M. Urbina
AU - S. R. Choi
AU - D. C. Cheung
AU - L. K. Egbert
AU - M. I. Garcia-Neuer
AU - S. Yu
AU - N. S. Rehman
AU - D. G. Hanks
AU - T.-A. Le
AU - H. H. Alabay
AU - K. E. McHugh
AU - F. H. Hamdan
AU - N. Naik
AU - W. A. Faubion
AU - J. T. Brady
AU - H. Ceylan
AU - S. Bose
PY - 2026
DA - 2026/09/17
PB - bioRxiv
DO - 10.64898/2026.09.17.752436
UR - https://www.biorxiv.org/content/10.64898/2026.09.17.752436v1
AB - Human intestinal diseases, including inflammatory bowel disease (IBD), are difficult to model because existing animal and in vitro systems do not capture the integrated vascular, immune, and metabolic complexity of the intestine. Here, we establish a custom normothermic machine perfusion platform that maintains surgically resected human intestinal tissue for up to 72 hours under near-physiological conditions. Discarded specimens from patients with nonmalignant intestinal diseases, including IBD and diverticulitis, were continuously perfused at 37{degrees}C with real-time hemodynamic and metabolic monitoring. Perfusion preserved tissue viability, mucosal architecture, epithelial integrity, oxygen consumption, metabolic activity, and vascular patency. Daily perfusate sampling enabled longitudinal profiling and experimental modulation of inflammatory mediators, revealing patient-specific immune states. This long-duration platform bridges reductionist in vitro models and clinical disease, enabling mechanistic studies of intestinal inflammation and future testing of personalized therapies in intact patient-derived tissue.
ER -