Heterologous Expression and Characterization of a Novel Polygalacturonase (DdpgA) from Dickeya dadantii DCE-01 and Its Application in Bioscouring of Ramie Fabric
- 1Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
- 2Hunan Lerkam Biology Co., Ltd., Yueyang 414100, China
Abstract
Efficient and eco-friendly bioscouring of ramie fabric is a critical step in the textile industry. To obtain polygalacturonase (DdpgA) suitable for the bioscouring of ramie fabric, a novel polygalacturonase gene (ddpgA) from the ramie-biodegumming strain Dickeya dadantii DCE-01 was subjected to bioinformatic analysis and heterologous expression. The recombinant plasmid pET28a-ddpgA was introduced into E. coli BL21(DE3), and the engineering strain BL21/pET28a-ddpgA was constructed; then induction conditions were optimized by response surface methodology. The recombinant DdpgA was purified and enzymatically characterized and further applied to the treatment of greige ramie fabric in combination with mannanase and xylanase. The results showed that the optimal pH of DdpgA was 5.0 and the optimal temperature was 55 °C; the enzyme was stable over the pH range of 3.0–6.0 and retained more than 50% of its activity after incubation at 55 °C for 60 min. DdpgA exhibited the highest catalytic activity toward sodium polygalacturonate. Mn2+ significantly activated DdpgA, whereas Ba2+, Pb2+, and Cu2+ inhibited its activity. Compound-enzyme scouring of ramie fabric formulated with DdpgA achieved a weight loss rate of 15.22%, a pectin removal rate of 51.28%, and a water-drop wetting time of less than 2 s; scanning electron microscopy confirmed the effective removal of gummy composites from the ramie fiber surface. This enzymatic scouring for ramie fabric avoids the strong alkali and high temperature required by conventional alkalic scouring, and thus represents an environmentally friendly alternative for ramie-fiber processing. Therefore, this study achieved high-level soluble expression of ddpgA in E. coli and obtained an acidic mesophilic polygalacturonase with demonstrated ramie bioscouring performance.
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BibTeX
@article{shi2026heterologous,
title = {{Heterologous Expression and Characterization of a Novel Polygalacturonase (DdpgA) from Dickeya dadantii DCE-01 and Its Application in Bioscouring of Ramie Fabric}},
author = {Ke Shi and Qi Yang and Guoguo Xi and Chen Chen and Ke Zheng and Yiyi Guo and Xiang Zhou and Qi Li and Tuo Lv and Shengwen Duan and Lifeng Cheng},
journal = {Polymers},
year = {2026},
month = oct,
doi = {10.3390/polym18212618},
url = {https://www.mdpi.com/2073-4360/18/21/2618},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Heterologous Expression and Characterization of a Novel Polygalacturonase (DdpgA) from Dickeya dadantii DCE-01 and Its Application in Bioscouring of Ramie Fabric
AU - Ke Shi
AU - Qi Yang
AU - Guoguo Xi
AU - Chen Chen
AU - Ke Zheng
AU - Yiyi Guo
AU - Xiang Zhou
AU - Qi Li
AU - Tuo Lv
AU - Shengwen Duan
AU - Lifeng Cheng
PY - 2026
DA - 2026/10/22
JO - Polymers
DO - 10.3390/polym18212618
UR - https://www.mdpi.com/2073-4360/18/21/2618
AB - Efficient and eco-friendly bioscouring of ramie fabric is a critical step in the textile industry. To obtain polygalacturonase (DdpgA) suitable for the bioscouring of ramie fabric, a novel polygalacturonase gene (ddpgA) from the ramie-biodegumming strain Dickeya dadantii DCE-01 was subjected to bioinformatic analysis and heterologous expression. The recombinant plasmid pET28a-ddpgA was introduced into E. coli BL21(DE3), and the engineering strain BL21/pET28a-ddpgA was constructed; then induction conditions were optimized by response surface methodology. The recombinant DdpgA was purified and enzymatically characterized and further applied to the treatment of greige ramie fabric in combination with mannanase and xylanase. The results showed that the optimal pH of DdpgA was 5.0 and the optimal temperature was 55 °C; the enzyme was stable over the pH range of 3.0–6.0 and retained more than 50% of its activity after incubation at 55 °C for 60 min. DdpgA exhibited the highest catalytic activity toward sodium polygalacturonate. Mn2+ significantly activated DdpgA, whereas Ba2+, Pb2+, and Cu2+ inhibited its activity. Compound-enzyme scouring of ramie fabric formulated with DdpgA achieved a weight loss rate of 15.22%, a pectin removal rate of 51.28%, and a water-drop wetting time of less than 2 s; scanning electron microscopy confirmed the effective removal of gummy composites from the ramie fiber surface. This enzymatic scouring for ramie fabric avoids the strong alkali and high temperature required by conventional alkalic scouring, and thus represents an environmentally friendly alternative for ramie-fiber processing. Therefore, this study achieved high-level soluble expression of ddpgA in E. coli and obtained an acidic mesophilic polygalacturonase with demonstrated ramie bioscouring performance.
SN - 2073-4360
ER -