Experimental and computational analyses of KEA3-dependent photosynthetic responses uncover species-specific…

Abstract

- NPQ relaxation is thought to limit crop biomass and yield under natural, fluctuating light. The thylakoid K/H exchanger KEA3 accelerates NPQ relaxation by lowering the lumen proton concentration and thus should affect biomass and yield under frequent light fluctuations. - To investigate the impact of KEA3-dependent NPQ relaxation on Solanum lycopersicum (tomato), we generated knock-out and over-expression lines and measured NPQ, gas exchange, biomass and yield. We constructed a mathematical model to compare species-specific NPQ relaxation dynamics in tomato and the model plant Arabidopsis thaliana. - In polytunnel and controlled fluctuating-light conditions, KEA3-mediated NPQ relaxation did not translate into consistent differences in tomato biomass or yield. Although KEA3 levels strongly affected NPQ relaxation, more so than in A.thaliana, the resulting effects on CO2 assimilation were minor. Modeling results indicated that, besides minor changes in KEA3 regulation, the activity of PsbS, the primary NPQ trigger, largely determines the species-specific differences in NPQ relaxation. Structural models from both species predict sequence variation with impact on PsbS activation. - Manipulating KEA3 levels in tomato did not link NPQ relaxation with productivity. However, the results support a robust cross-species mathematical model that accurately predicts the molecular interactions governing NPQ dynamics.

Concentration-Dependent Modes of Melittin-Induced Membrane Destabilization: An Integration of Experimental and Computational Tools.
Andrea Chacón Calderón, Pavel Zelenovskii, Márcio Soares +7
Langmuir : the ACS journal of surfaces and colloids1 Sep 2026Peer-reviewedCC BYFull textMicrobiologyEurope PMC
Dynamic Responses of Photosynthetic Parameters in Lonicera japonica Leaves During Photosynthetic Induction Under Elevated CO2 Concentrations
Erli Tian, Yongchuang Liu, Mengdi Mu +2
Plants (Basel, Switzerland)24 Sep 2026Peer-reviewedCC BYFull textPlant biologyEurope PMC
DIFFERENTIAL PHOTOSYNTHETIC RESPONSES TO GLUFOSINATE AMMONIUM IN TWO GRASS WEEDS: Lolium multiflorum AND Echinochloa crus-galli.
O. Sabatini, J. Villalba, S. R. Simondi +2
bioRxiv31 Aug 2026PreprintCC BY-NC-NDFull textPlant biologybioRxiv
Photosynthetic and Antioxidant Responses of Lolium perenne to Aluminum Oxide Nanoparticles, Their Supernatant, and Aluminum Chloride Under Acidic Conditions
Jorge González-Villagra, Valentina Figueroa, Paz Cárcamo-Fincheira +6
Molecules1 Oct 2026Peer-reviewedCC BY 4.0Full textPlant biologyCrossrefEurope PMC
Tissue-resolved photosynthetic responses in living leaves revealed by microscopic imaging-pulse-amplitude-modulation
Y. Kato, K. Takeuchi, S. Harimoto +2
bioRxiv9 Sep 2026PreprintCC BY-NC-NDFull textPlant biologybioRxiv

Cite

BibTeX RIS

BibTeX

@misc{bismarck2026experimental,
  title = {{Experimental and computational analyses of KEA3-dependent photosynthetic responses uncover species-specific molecular variation}},
  author = {T. von Bismarck and Q. H. Nguyen and J. Russ and L. Strandberg and M. Krämer and J. Damen and H.-H. Kunz and P. Jahns and K. Köhl and A. Matuszynska and U. Armbruster},
  howpublished = {bioRxiv},
  year = {2026},
  month = oct,
  doi = {10.64898/2026.10.13.758443},
  url = {https://www.biorxiv.org/content/10.64898/2026.10.13.758443v1},
  note = {CC BY-NC},
}

RIS

TY  - UNPB
TI  - Experimental and computational analyses of KEA3-dependent photosynthetic responses uncover species-specific molecular variation
AU  - T. von Bismarck
AU  - Q. H. Nguyen
AU  - J. Russ
AU  - L. Strandberg
AU  - M. Krämer
AU  - J. Damen
AU  - H.-H. Kunz
AU  - P. Jahns
AU  - K. Köhl
AU  - A. Matuszynska
AU  - U. Armbruster
PY  - 2026
DA  - 2026/10/13
PB  - bioRxiv
DO  - 10.64898/2026.10.13.758443
UR  - https://www.biorxiv.org/content/10.64898/2026.10.13.758443v1
AB  - - NPQ relaxation is thought to limit crop biomass and yield under natural, fluctuating light. The thylakoid K/H exchanger KEA3 accelerates NPQ relaxation by lowering the lumen proton concentration and thus should affect biomass and yield under frequent light fluctuations. - To investigate the impact of KEA3-dependent NPQ relaxation on Solanum lycopersicum (tomato), we generated knock-out and over-expression lines and measured NPQ, gas exchange, biomass and yield. We constructed a mathematical model to compare species-specific NPQ relaxation dynamics in tomato and the model plant Arabidopsis thaliana. - In polytunnel and controlled fluctuating-light conditions, KEA3-mediated NPQ relaxation did not translate into consistent differences in tomato biomass or yield. Although KEA3 levels strongly affected NPQ relaxation, more so than in A.thaliana, the resulting effects on CO2 assimilation were minor. Modeling results indicated that, besides minor changes in KEA3 regulation, the activity of PsbS, the primary NPQ trigger, largely determines the species-specific differences in NPQ relaxation. Structural models from both species predict sequence variation with impact on PsbS activation. - Manipulating KEA3 levels in tomato did not link NPQ relaxation with productivity. However, the results support a robust cross-species mathematical model that accurately predicts the molecular interactions governing NPQ dynamics.
ER  -

Abstract

Related

Cite