Rapid and largely reversible shifts in the canine fecal metabolome during dietary change
- 1Szegedi Tudomanyegyetem Altalanos Orvostudomanyi Kar
Abstract
Diet can rapidly change the fecal metabolome, but less is known about recovery after the original diet is restored. We used untargeted UPLC-MS metabolomics to analyze 72 fecal samples from nine Pumi dogs during an owner-managed switch from dry food to raw food and back to dry food. Diet phase accounted for a large proportion of variation in both ionization modes. More than 13,000 LC-MS features changed at the first sampling point after the switch to raw food, with a similarly large response after return to dry food. Among features significant in both comparisons, more than 99% changed in opposite directions. At the final sampling point, no positive-mode (ESI+) features and only 13 negative-mode (ESI-) features differed from the second dry-food baseline under the same threshold. BARF-associated patterns persisted in analyses excluding individual dogs and in pedigree-adjusted candidate models, although individual feature effects depended on normalization. Putative metabolites from several biochemical classes differed in their response and recovery. The fecal metabolome therefore changed rapidly and returned largely toward baseline, with differences among dogs.
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BibTeX
@misc{jaray2026rapid,
title = {{Rapid and largely reversible shifts in the canine fecal metabolome during dietary change}},
author = {T. Jaray and Z. Csabai and Z. Boldogkoi and D. Tombacz},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.26.754668},
url = {https://www.biorxiv.org/content/10.64898/2026.09.26.754668v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Rapid and largely reversible shifts in the canine fecal metabolome during dietary change
AU - T. Jaray
AU - Z. Csabai
AU - Z. Boldogkoi
AU - D. Tombacz
PY - 2026
DA - 2026/09/26
PB - bioRxiv
DO - 10.64898/2026.09.26.754668
UR - https://www.biorxiv.org/content/10.64898/2026.09.26.754668v1
AB - Diet can rapidly change the fecal metabolome, but less is known about recovery after the original diet is restored. We used untargeted UPLC-MS metabolomics to analyze 72 fecal samples from nine Pumi dogs during an owner-managed switch from dry food to raw food and back to dry food. Diet phase accounted for a large proportion of variation in both ionization modes. More than 13,000 LC-MS features changed at the first sampling point after the switch to raw food, with a similarly large response after return to dry food. Among features significant in both comparisons, more than 99% changed in opposite directions. At the final sampling point, no positive-mode (ESI+) features and only 13 negative-mode (ESI-) features differed from the second dry-food baseline under the same threshold. BARF-associated patterns persisted in analyses excluding individual dogs and in pedigree-adjusted candidate models, although individual feature effects depended on normalization. Putative metabolites from several biochemical classes differed in their response and recovery. The fecal metabolome therefore changed rapidly and returned largely toward baseline, with differences among dogs.
ER -