Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters
Abstract
Cell culture-conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM-derived EVs (CCM-EV). The CCM-EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell-specific recommendations may need to be established for non-mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM-EV research.
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BibTeX
@article{shekari2026cell,
title = {{Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters}},
author = {Faezeh Shekari and Faisal J Alibhai and Hossein Baharvand and Verena Börger and Stefania Bruno and Owen Davies and Bernd Giebel and Mario Gimona and Ghasem Hosseini Salekdeh and Lorena Martin‐jaular and Suresh Mathivanan and Inge Nelissen and Esther Nolte‐’t Hoen and Lorraine O'Driscoll and Francesca Perut and Stefano Pluchino and Gabriella Pocsfalvi and Carlos Salomon and Carolina Soekmadji and Simon Staubach and Ana Claudia Torrecilhas and Ganesh Vilas Shelke and Tobias Tertel and Dandan Zhu and Clotilde Théry and Kenneth Witwer and Rienk Nieuwland},
journal = {Journal of Extracellular Biology},
year = {2026},
month = oct,
doi = {10.1002/jex2.115},
url = {https://inserm.hal.science/inserm-05779201v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters
AU - Faezeh Shekari
AU - Faisal J Alibhai
AU - Hossein Baharvand
AU - Verena Börger
AU - Stefania Bruno
AU - Owen Davies
AU - Bernd Giebel
AU - Mario Gimona
AU - Ghasem Hosseini Salekdeh
AU - Lorena Martin‐jaular
AU - Suresh Mathivanan
AU - Inge Nelissen
AU - Esther Nolte‐’t Hoen
AU - Lorraine O'Driscoll
AU - Francesca Perut
AU - Stefano Pluchino
AU - Gabriella Pocsfalvi
AU - Carlos Salomon
AU - Carolina Soekmadji
AU - Simon Staubach
AU - Ana Claudia Torrecilhas
AU - Ganesh Vilas Shelke
AU - Tobias Tertel
AU - Dandan Zhu
AU - Clotilde Théry
AU - Kenneth Witwer
AU - Rienk Nieuwland
PY - 2026
DA - 2026/10/05
JO - Journal of Extracellular Biology
DO - 10.1002/jex2.115
UR - https://inserm.hal.science/inserm-05779201v1
AB - Cell culture-conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM-derived EVs (CCM-EV). The CCM-EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell-specific recommendations may need to be established for non-mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM-EV research.
SN - 2768-2811
ER -