Waste-Contaminated Sites Near Dhaka City Harbour Specific Biosurfactant-Producing Bacillus and Stutzerimonas Strains With Potential Biotechnological Applications.
- 1Department of Life Sciences, School of Environment and Life Sciences, Independent University Bangladesh, Dhaka, Bangladesh.
- 2IUB Plasma Plus Research and Testing Laboratory, School of Pharmacy and Public Health, Independent University Bangladesh, Dhaka, Bangladesh.
- 3The Center for Computational and Data Sciences, Independent University Bangladesh, Dhaka, Bangladesh.
Abstract
Biosurfactants are eco-friendly surface-active compounds produced by microorganisms that lower surface and interfacial tensions, enhancing the bioavailability of hydrophobic compounds. The study reports the isolation and characterization of biosurfactant-producing bacteria from industrial waste effluent in a river near Dhaka, Bangladesh. Using diesel-enriched media, bacterial strains were isolated and screened for biosurfactant production using oil displacement, emulsification index (E24%), and drop-collapse assays. The microbial cultures were characterized based on biochemical activities and 16S rRNA sequencing. Biosurfactant characterization was carried out using chromatographic techniques. Six cultures passed the drop-collapse and oil-displacement tests with E24% ranging from 9% to 41%. The CTAB agar assay confirmed the anionic nature of the biosurfactants. The potential biosurfactant producers belong mostly to the Bacillus and Stutzerimonas groups, with one low-biosurfactant-producing isolate from the Enterobacteriaceae group. The presence of two consortia, Bacillus-Acinetobacter and Bacillus-Stutzerimonas, was also observed. The isolates and consortia exhibited varying levels of antibacterial, antifungal, adherence, and plant growth-promoting activity. Biosurfactant production capacity under extreme conditions has been characterized by pH stability (pH 2-pH 11), salinity tolerance (2%-10% NaCl), and temperature tolerance (40°C-100°C). Overall, they provide a green and economical alternative to synthetic surfactants, with low toxicity and potential applications in bioremediation, biocontrol, and plant growth promotion.
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BibTeX
@article{amina2026wastecontaminated,
title = {{Waste-Contaminated Sites Near Dhaka City Harbour Specific Biosurfactant-Producing Bacillus and Stutzerimonas Strains With Potential Biotechnological Applications.}},
author = {Ummey Fatema Tun Amina and Maliha Mahzabin and Sk Shahriar Bin Rasul and Sabrina M Elias},
journal = {Environmental microbiology reports},
year = {2026},
month = oct,
doi = {10.1111/1758-2229.70440},
url = {https://europepmc.org/article/MED/42924592},
note = {CC BY},
}
RIS
TY - JOUR
TI - Waste-Contaminated Sites Near Dhaka City Harbour Specific Biosurfactant-Producing Bacillus and Stutzerimonas Strains With Potential Biotechnological Applications.
AU - Ummey Fatema Tun Amina
AU - Maliha Mahzabin
AU - Sk Shahriar Bin Rasul
AU - Sabrina M Elias
PY - 2026
DA - 2026/10/01
JO - Environmental microbiology reports
DO - 10.1111/1758-2229.70440
UR - https://europepmc.org/article/MED/42924592
AB - Biosurfactants are eco-friendly surface-active compounds produced by microorganisms that lower surface and interfacial tensions, enhancing the bioavailability of hydrophobic compounds. The study reports the isolation and characterization of biosurfactant-producing bacteria from industrial waste effluent in a river near Dhaka, Bangladesh. Using diesel-enriched media, bacterial strains were isolated and screened for biosurfactant production using oil displacement, emulsification index (E24%), and drop-collapse assays. The microbial cultures were characterized based on biochemical activities and 16S rRNA sequencing. Biosurfactant characterization was carried out using chromatographic techniques. Six cultures passed the drop-collapse and oil-displacement tests with E24% ranging from 9% to 41%. The CTAB agar assay confirmed the anionic nature of the biosurfactants. The potential biosurfactant producers belong mostly to the Bacillus and Stutzerimonas groups, with one low-biosurfactant-producing isolate from the Enterobacteriaceae group. The presence of two consortia, Bacillus-Acinetobacter and Bacillus-Stutzerimonas, was also observed. The isolates and consortia exhibited varying levels of antibacterial, antifungal, adherence, and plant growth-promoting activity. Biosurfactant production capacity under extreme conditions has been characterized by pH stability (pH 2-pH 11), salinity tolerance (2%-10% NaCl), and temperature tolerance (40°C-100°C). Overall, they provide a green and economical alternative to synthetic surfactants, with low toxicity and potential applications in bioremediation, biocontrol, and plant growth promotion.
SN - 1758-2229
ER -