Collagen‐Based Gut‐on‐Chip for In Vitro Modeling of Intestinal Barrier Function and Host‐Pathogen Interactions
Abstract
The human intestine, characterized by villi and crypt structures, is central to nutrient absorption, barrier function, and host defense. Traditional in vitro models using synthetic membranes such as polydimethylsiloxane (PDMS) and polycarbonate (PC) often fail to accurately replicate the complex intestinal environment. To overcome this, we developed a gut‐on‐chip (GOC), incorporating a thin, porous collagen type I membrane, to better mimic the natural extracellular matrix (ECM) and create a more physiologically relevant system. Membranes were fabricated and characterized by profilometry to determine their thickness, which closely approximates the in vivo intestinal basement membrane. Caco‐2 cells cultured within the device exhibit villi‐like structures, form tight junctions, produce mucin, and establish a functional barrier, demonstrating rapid and stable differentiation on the collagen membrane. We further investigated the device's capacity to model host‐pathogen interactions by infecting the epithelial layer with Candida albicans . Confocal microscopy reveals hyphal invasion of the epithelial cells, while permeability assays demonstrate increased barrier permeability, highlighting the device's ability to replicate infection processes and their impact on barrier integrity. By integrating a physiological membrane and replicating key structural and functional features of the intestine, this GOC provides a promising biomimetic platform for studying intestinal physiology and host‐pathogen dynamics.
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BibTeX
@article{garcia2026collagenbased,
title = {{Collagen‐Based Gut‐on‐Chip for In Vitro Modeling of Intestinal Barrier Function and Host‐Pathogen Interactions}},
author = {Fernanda López García and Jacques Leng and Hugo de Oliveira and Pierre Nassoy and Karine Dementhon},
journal = {Advanced Materials Technologies},
year = {2026},
month = jul,
doi = {10.1002/admt.71187},
url = {https://hal.science/hal-05842155v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Collagen‐Based Gut‐on‐Chip for In Vitro Modeling of Intestinal Barrier Function and Host‐Pathogen Interactions
AU - Fernanda López García
AU - Jacques Leng
AU - Hugo de Oliveira
AU - Pierre Nassoy
AU - Karine Dementhon
PY - 2026
DA - 2026/07/31
JO - Advanced Materials Technologies
DO - 10.1002/admt.71187
UR - https://hal.science/hal-05842155v1
AB - The human intestine, characterized by villi and crypt structures, is central to nutrient absorption, barrier function, and host defense. Traditional in vitro models using synthetic membranes such as polydimethylsiloxane (PDMS) and polycarbonate (PC) often fail to accurately replicate the complex intestinal environment. To overcome this, we developed a gut‐on‐chip (GOC), incorporating a thin, porous collagen type I membrane, to better mimic the natural extracellular matrix (ECM) and create a more physiologically relevant system. Membranes were fabricated and characterized by profilometry to determine their thickness, which closely approximates the in vivo intestinal basement membrane. Caco‐2 cells cultured within the device exhibit villi‐like structures, form tight junctions, produce mucin, and establish a functional barrier, demonstrating rapid and stable differentiation on the collagen membrane. We further investigated the device's capacity to model host‐pathogen interactions by infecting the epithelial layer with Candida albicans . Confocal microscopy reveals hyphal invasion of the epithelial cells, while permeability assays demonstrate increased barrier permeability, highlighting the device's ability to replicate infection processes and their impact on barrier integrity. By integrating a physiological membrane and replicating key structural and functional features of the intestine, this GOC provides a promising biomimetic platform for studying intestinal physiology and host‐pathogen dynamics.
SN - 2365-709X
ER -