TMT-based quantitative proteomic analysis of vitreous humor in proliferative diabetic retinopathy.
4 affiliations
- 1Meishan Hospital, West China Hospital Sichuan University, Meishan, 620010, China.
- 2Department of Ophthalmology, Meishan People's Hospital, Meishan, 620010, China.
- 3Department of Ophthalmology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
- 4Department of Ophthalmology, The Affiliated Hospital of Southwest Medical University, Luzhou, China. [email protected].
Abstract
Objective
To study the changes in vitreous humor protein profile of proliferative diabetic retinopathy (PDR) and macular hole (MH)/epimacular membrane (EMM), and to identify potential candidate biomarkers for PDR by screening differentially expressed proteins (DEPs).Methods
This study used tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify DEPs in vitreous samples from PDR patients and MH/EMM control patients. The identified DEPs were analyzed via bioinformatics methods to screen candidate proteins with research potential. Parallel reaction monitoring (PRM) technology was used to perform targeted validation of 30 proteins with potential regulatory effects in PDR.Results
A total of 4812 peptides and 877 proteins were isolated and identified via TMT combined with LC-MS/MS proteomic analysis, including 59 DEPs (fold change > 1.2, P < 0.05). Bioinformatics analysis showed that DEPs were mainly involved in critical biological processes including cellular process, stimulus-response, biological regulation, localization, immune system process and metabolic process. A total of 23 proteins were successfully quantified via PRM validation, and the overall expression trends were consistent between TMT-based quantification and PRM results. Among them, coagulation factor V (FV), profilin-1 (PFN1), thioredoxin (TXN), ferritin light chain (FTL) and selenoprotein P (SeP) not only showed consistent and significant quantitative trends, but also reached statistical significance (P < 0.05) in both discovery and validation cohorts.Conclusion
This study revealed significant alterations in the vitreous humor protein profile of PDR patients. The five identified candidate proteins (FV, PFN1, TXN, FTL, SeP) may serve as potential candidate biomarkers for PDR, providing new research directions and therapeutic targets for exploring the pathogenesis of PDR.
Related
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BibTeX
@article{xiong2026tmtbased,
title = {{TMT-based quantitative proteomic analysis of vitreous humor in proliferative diabetic retinopathy.}},
author = {Xin Xiong and Qi Zhou and Yang Cao and Min Tang and Like Xie and Hongbin Lv},
journal = {BMC ophthalmology},
year = {2026},
month = nov,
doi = {10.1186/s12886-026-04847-0},
url = {https://europepmc.org/article/MED/42968080},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - TMT-based quantitative proteomic analysis of vitreous humor in proliferative diabetic retinopathy.
AU - Xin Xiong
AU - Qi Zhou
AU - Yang Cao
AU - Min Tang
AU - Like Xie
AU - Hongbin Lv
PY - 2026
DA - 2026/11/02
JO - BMC ophthalmology
DO - 10.1186/s12886-026-04847-0
UR - https://europepmc.org/article/MED/42968080
AB - Objective To study the changes in vitreous humor protein profile of proliferative diabetic retinopathy (PDR) and macular hole (MH)/epimacular membrane (EMM), and to identify potential candidate biomarkers for PDR by screening differentially expressed proteins (DEPs).Methods This study used tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify DEPs in vitreous samples from PDR patients and MH/EMM control patients. The identified DEPs were analyzed via bioinformatics methods to screen candidate proteins with research potential. Parallel reaction monitoring (PRM) technology was used to perform targeted validation of 30 proteins with potential regulatory effects in PDR.Results A total of 4812 peptides and 877 proteins were isolated and identified via TMT combined with LC-MS/MS proteomic analysis, including 59 DEPs (fold change > 1.2, P < 0.05). Bioinformatics analysis showed that DEPs were mainly involved in critical biological processes including cellular process, stimulus-response, biological regulation, localization, immune system process and metabolic process. A total of 23 proteins were successfully quantified via PRM validation, and the overall expression trends were consistent between TMT-based quantification and PRM results. Among them, coagulation factor V (FV), profilin-1 (PFN1), thioredoxin (TXN), ferritin light chain (FTL) and selenoprotein P (SeP) not only showed consistent and significant quantitative trends, but also reached statistical significance (P < 0.05) in both discovery and validation cohorts.Conclusion This study revealed significant alterations in the vitreous humor protein profile of PDR patients. The five identified candidate proteins (FV, PFN1, TXN, FTL, SeP) may serve as potential candidate biomarkers for PDR, providing new research directions and therapeutic targets for exploring the pathogenesis of PDR.
SN - 1471-2415
ER -