Small viruses reveal bidirectional evolution between HK97-fold viruses and encapsulins via procapsids
Abstract
The HK97-fold is an ancient, conserved protein structure that assembles protein shells, forming the capsids of Duplodnaviria viruses and prokaryotic biochemical compartments called encapsulsins. It has been proposed that encapsulins evolved from HK97-fold viruses. But the higher complexity of viral capsids represents a biophysical paradox in the evolution of HK97-fold protein shells. To resolve this problem, we hypothesize the existence of simpler HK97-fold viral capsids associated with smaller genomes than those of known HK97-fold viruses. Such small viral genomes are searched in environmental metagenomes across public databases, including IMG/M and MGnify. Using hidden Markov models and protein language models, we identify and characterize small viral genomes encoding HK97-fold proteins that exhibit molecular similarities to encapsulins yet lack portal and tail genes. The phylogenetic analyses of HK97-fold proteins reveal independent mixed groups of viruses and encapsulins, and structural analyses identify similarities between encapsulins and procapsids, the immature state of viral capsids. The observations support a bidirectional evolution between encapsulins and HK97-fold viruses enabled by the viral procapsid state. Using biophysical and genomic arguments, we introduce an evolutionary model of HK97-fold protein shells, predicting that they emerged as lower-symmetry biochemical compartments and that HK97-fold viral capsids may retain biochemical functions.
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BibTeX
@article{aguilar2026small,
title = {{Small viruses reveal bidirectional evolution between HK97-fold viruses and encapsulins via procapsids}},
author = {Abelardo Aguilar and Ingrid Miranda Perez and Omer Nadel and Stephen Nayfach and Luis Ruiz Pestana and Anca M. Segall and Forest L. Rohwer and Simon Roux and Simon White and Antoni Luque},
journal = {Nature Communications},
year = {2026},
month = oct,
doi = {10.1038/s41467-026-78136-1},
url = {https://www.nature.com/articles/s41467-026-78136-1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Small viruses reveal bidirectional evolution between HK97-fold viruses and encapsulins via procapsids
AU - Abelardo Aguilar
AU - Ingrid Miranda Perez
AU - Omer Nadel
AU - Stephen Nayfach
AU - Luis Ruiz Pestana
AU - Anca M. Segall
AU - Forest L. Rohwer
AU - Simon Roux
AU - Simon White
AU - Antoni Luque
PY - 2026
DA - 2026/10/14
JO - Nature Communications
DO - 10.1038/s41467-026-78136-1
UR - https://www.nature.com/articles/s41467-026-78136-1
AB - The HK97-fold is an ancient, conserved protein structure that assembles protein shells, forming the capsids of Duplodnaviria viruses and prokaryotic biochemical compartments called encapsulsins. It has been proposed that encapsulins evolved from HK97-fold viruses. But the higher complexity of viral capsids represents a biophysical paradox in the evolution of HK97-fold protein shells. To resolve this problem, we hypothesize the existence of simpler HK97-fold viral capsids associated with smaller genomes than those of known HK97-fold viruses. Such small viral genomes are searched in environmental metagenomes across public databases, including IMG/M and MGnify. Using hidden Markov models and protein language models, we identify and characterize small viral genomes encoding HK97-fold proteins that exhibit molecular similarities to encapsulins yet lack portal and tail genes. The phylogenetic analyses of HK97-fold proteins reveal independent mixed groups of viruses and encapsulins, and structural analyses identify similarities between encapsulins and procapsids, the immature state of viral capsids. The observations support a bidirectional evolution between encapsulins and HK97-fold viruses enabled by the viral procapsid state. Using biophysical and genomic arguments, we introduce an evolutionary model of HK97-fold protein shells, predicting that they emerged as lower-symmetry biochemical compartments and that HK97-fold viral capsids may retain biochemical functions.
SN - 2041-1723
ER -