Dual-Loaded Quercetin-Resveratrol Niosomes: Sustained In Vitro Release and Retained Bioactivity in Hummus During Simulated Gastrointestinal Digestion.
4 affiliations
- 1Departamento de Ciencias de la Salud, Centro Universitario de los Valles (CUValles), Universidad de Guadalajara, Carretera a Guadalajara Km 45.5, Ameca 46600, Jalisco, Mexico.
- 2Departamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega (CUCIÉNEGA), Universidad de Guadalajara, Av. Universidad 1115, Lindavista, Ocotlán 47820, Jalisco, Mexico.
- 3Departamento de Investigación y Posgrado en Alimentos (DIPA), Universidad de Sonora, Blvd. Luis Encinas y Rosales S/N, Col. Centro, Hermosillo 83000, Sonora, Mexico.
- 4Departamento de Ecología Aplicada, Centro Universitario de Ciencias Biológicas y Agropecuarias (CUCBA), Universidad de Guadalajara, Camino Ing. Ramón Padilla Sánchez No. 2100, Las Agujas, Zapopan 45510, Jalisco, Mexico.
Abstract
Quercetin (QUE) and resveratrol (RES) have complementary bioactivities but limited aqueous solubility and oral bioavailability. This study developed dual-loaded quercetin-resveratrol niosomes (QR-NIOs) for incorporation into hummus and screened quercetin:resveratrol molar ratios using antioxidant, erythroprotective, and enzyme-inhibition assays. The 50:50 formulation showed the broadest activity profile and produced vesicles with 90.63 ± 3.63% overall encapsulation efficiency, 149 ± 13 nm diameter, 0.21 ± 0.02 polydispersity index, and -34 ± 2 mV ζ-potential. After 24 h, dialysis passage was 51.63%, compared with 80.35% for the unencapsulated mixture. Following simulated intestinal digestion, enriched hummus yielded 5.8 milligrams of gallic acid equivalents per gram of dry weight (mg GAE/g D.W.) and 4.7 milligrams of quercetin equivalents per gram of dry weight (mg QE/g D.W.) in the dialyzable phenolic and flavonoid fractions, respectively, exceeding both controls while retaining antioxidant and enzyme-inhibitory activities. Over 28 days at 4 °C, microbial counts remained below detection limits and the tested pathogens were absent. Overall acceptability (8.23/9) did not differ significantly from commercial hummus. QR-NIO-enriched hummus represents a sensory-compatible functional-food prototype with sustained apparent release and increased intestinal recovery of assay-reactive phenolic and flavonoid compounds; compound-specific and in vivo validation remains necessary.
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BibTeX
@article{miramontespiedra2026dualloaded,
title = {{Dual-Loaded Quercetin-Resveratrol Niosomes: Sustained In Vitro Release and Retained Bioactivity in Hummus During Simulated Gastrointestinal Digestion.}},
author = {Jade Alejandra Miramontes-Piedra and Miguel Ángel Robles-García and Alejandra Vázquez-Aguilar and Brenda Vega-Ruiz and Carmen Lizette Del-Toro-Sánchez and Fridha Viridiana Villalpando-Vargas and Sergio Yair Rodríguez-Preciado and Mariana Díaz-Zaragoza and Juan Carlos Barrera-de-León and Ernesto Ramírez-Briones and Ricardo Iván González-Vega},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198686},
url = {https://europepmc.org/article/MED/42883931},
note = {CC BY},
}
RIS
TY - JOUR
TI - Dual-Loaded Quercetin-Resveratrol Niosomes: Sustained In Vitro Release and Retained Bioactivity in Hummus During Simulated Gastrointestinal Digestion.
AU - Jade Alejandra Miramontes-Piedra
AU - Miguel Ángel Robles-García
AU - Alejandra Vázquez-Aguilar
AU - Brenda Vega-Ruiz
AU - Carmen Lizette Del-Toro-Sánchez
AU - Fridha Viridiana Villalpando-Vargas
AU - Sergio Yair Rodríguez-Preciado
AU - Mariana Díaz-Zaragoza
AU - Juan Carlos Barrera-de-León
AU - Ernesto Ramírez-Briones
AU - Ricardo Iván González-Vega
PY - 2026
DA - 2026/09/28
JO - International journal of molecular sciences
DO - 10.3390/ijms27198686
UR - https://europepmc.org/article/MED/42883931
AB - Quercetin (QUE) and resveratrol (RES) have complementary bioactivities but limited aqueous solubility and oral bioavailability. This study developed dual-loaded quercetin-resveratrol niosomes (QR-NIOs) for incorporation into hummus and screened quercetin:resveratrol molar ratios using antioxidant, erythroprotective, and enzyme-inhibition assays. The 50:50 formulation showed the broadest activity profile and produced vesicles with 90.63 ± 3.63% overall encapsulation efficiency, 149 ± 13 nm diameter, 0.21 ± 0.02 polydispersity index, and -34 ± 2 mV ζ-potential. After 24 h, dialysis passage was 51.63%, compared with 80.35% for the unencapsulated mixture. Following simulated intestinal digestion, enriched hummus yielded 5.8 milligrams of gallic acid equivalents per gram of dry weight (mg GAE/g D.W.) and 4.7 milligrams of quercetin equivalents per gram of dry weight (mg QE/g D.W.) in the dialyzable phenolic and flavonoid fractions, respectively, exceeding both controls while retaining antioxidant and enzyme-inhibitory activities. Over 28 days at 4 °C, microbial counts remained below detection limits and the tested pathogens were absent. Overall acceptability (8.23/9) did not differ significantly from commercial hummus. QR-NIO-enriched hummus represents a sensory-compatible functional-food prototype with sustained apparent release and increased intestinal recovery of assay-reactive phenolic and flavonoid compounds; compound-specific and in vivo validation remains necessary.
SN - 1422-0067
ER -