NLRC3 deficiency exacerbates high-fat diet-induced enteric nervous system injury and colonic motility dysfunction in mice via activating NF-κB signaling pathway
5 affiliations
- 1Department of Human Anatomy, Basic Medical College, Guangxi Medical University, Nanning, 530000 China
- 2Department of Anatomy and K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi’an, 710032 China
- 3College of Life Science, Northwest University, Xi’an, 710069 China
- 4Department of Endocrinology, Xijing Hospital, The Fourth Medical University, Xi’an, 710038 China
- 5Department of Human Anatomy, Basic Medical College, Zunyi Medical University, Zunyi, 563006 China
Abstract
A high-fat diet (HFD) induces low-grade intestinal inflammation, thereby causing structural and functional impairments of the enteric nervous system (ENS). Nucleotide oligomerization domain (NOD)-like receptor (NLR) containing a caspase activation and recruitment domain 3 (NLRC3) participates in key pathophysiological processes related to inflammation, immunity, and proliferative diseases. However, the function of NLRC3 within the ENS remains to be clarified. This investigation was designed to characterize NLRC3 expression and distribution within the ENS and to elucidate its mechanism in modulating ENS injury. An HFD-induced low-grade colonic inflammation model was established in NLRC3 gene knockout (Nlrc3−/−) mice and wild-type (WT) C57BL/6J mice. The influence of NLRC3 on ENS architecture and motor function was assessed using morphological evaluation, molecular biological assays, and colonic motility analyses. The findings demonstrated that NLRC3 was primarily distributed within the cytoplasm of ENS neurons in mice, with HFD decreasing NLRC3 expression in enteric neurons. Relative to WT mice, HFD-treated Nlrc3−/− mice developed more pronounced ENS injury, characterized by enteric glial cells proliferation and activation, enteric neuronal loss accompanied by structural disruption, and a further delay in colonic transit function. NLRC3 deficiency exacerbated HFD-induced ENS injury and enteric neuronal apoptosis via nuclear factor-κB signaling pathway activation. These observations establish NLRC3 as a key negative regulator of neuroinflammatory responses in the ENS. As a negative regulatory pattern recognition receptor that links innate immunity with ENS signaling, NLRC3 may represent a promising therapeutic target.
Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s00018-026-06364-1.
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BibTeX
@article{wang2026nlrc3,
title = {{NLRC3 deficiency exacerbates high-fat diet-induced enteric nervous system injury and colonic motility dysfunction in mice via activating NF-κB signaling pathway}},
author = {Yan Wang and Yan Li and Meng-Ying Li and Sha-Sha Tao and Guo-He Tan and Yun-Qing Li},
journal = {Cellular and Molecular Life Sciences: CMLS},
year = {2026},
month = jul,
doi = {10.1007/s00018-026-06364-1},
url = {https://doi.org/10.1007/s00018-026-06364-1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - NLRC3 deficiency exacerbates high-fat diet-induced enteric nervous system injury and colonic motility dysfunction in mice via activating NF-κB signaling pathway
AU - Yan Wang
AU - Yan Li
AU - Meng-Ying Li
AU - Sha-Sha Tao
AU - Guo-He Tan
AU - Yun-Qing Li
PY - 2026
DA - 2026/07/27
JO - Cellular and Molecular Life Sciences: CMLS
DO - 10.1007/s00018-026-06364-1
UR - https://doi.org/10.1007/s00018-026-06364-1
AB - A high-fat diet (HFD) induces low-grade intestinal inflammation, thereby causing structural and functional impairments of the enteric nervous system (ENS). Nucleotide oligomerization domain (NOD)-like receptor (NLR) containing a caspase activation and recruitment domain 3 (NLRC3) participates in key pathophysiological processes related to inflammation, immunity, and proliferative diseases. However, the function of NLRC3 within the ENS remains to be clarified. This investigation was designed to characterize NLRC3 expression and distribution within the ENS and to elucidate its mechanism in modulating ENS injury. An HFD-induced low-grade colonic inflammation model was established in NLRC3 gene knockout (Nlrc3−/−) mice and wild-type (WT) C57BL/6J mice. The influence of NLRC3 on ENS architecture and motor function was assessed using morphological evaluation, molecular biological assays, and colonic motility analyses. The findings demonstrated that NLRC3 was primarily distributed within the cytoplasm of ENS neurons in mice, with HFD decreasing NLRC3 expression in enteric neurons. Relative to WT mice, HFD-treated Nlrc3−/− mice developed more pronounced ENS injury, characterized by enteric glial cells proliferation and activation, enteric neuronal loss accompanied by structural disruption, and a further delay in colonic transit function. NLRC3 deficiency exacerbated HFD-induced ENS injury and enteric neuronal apoptosis via nuclear factor-κB signaling pathway activation. These observations establish NLRC3 as a key negative regulator of neuroinflammatory responses in the ENS. As a negative regulatory pattern recognition receptor that links innate immunity with ENS signaling, NLRC3 may represent a promising therapeutic target. Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s00018-026-06364-1.
SN - 1420-682X
SN - 1420-9071
ER -