Laboratory diagnosis of the first case of Type E botulism caused by Clostridium butyricum in Xinjiang
- 1Center for Disease Prevention and Control of Xinjiang Uygur Autonomous Region (Xinjiang Uygur Autonomous Region Academy of Preventive Medicine), Urumqi, Xinjiang 830002, China
Abstract
ObjectiveTo conduct laboratory testing on the sample associated with a suspected botulism incident in Xinjiang in February 2025.MethodsThe patient’s fecal specimen was pre-treated in accordance with Chinese health industry standard WS 271-2007. The fecal suspension was inoculated into cooked-meat medium and trypticase peptone glucose yeast extract-trypsin medium （TPGYT）for enrichment culture. Real-time fluorescence quantitative PCR was used to detect botulinum toxin genes in the enrichment broth. Isolation and purification were performed on blood agar， followed by species identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry （MALDI-TOF MS） and whole-genome sequencing. Toxin production by the isolate was confirmed by mouse bioassay.ResultsThe bont/E gene was detected positive by real-time fluorescence PCR in the enriched culture of the patient’s fecal specimen， while type A， B and F bont genes were all negative. One strain with grayish-white， flattened， irregular-edged colonies was isolated on blood agar plates and identified as Clostridium butyricum by MALDI-TOF MS （confidence score 9.542）. Average nucleotide identity （ANI） analysis based on whole-genome sequences showed that this strain shared the highest ANI value of 98.90% with C. butyricum NBRC 13949， with a genome coverage of 90.31%. The bont/E gene carried by the isolate exhibited 100% sequence similarity and 99.92% coverage with the same gene from C. butyricum 5521. The mouse bioassay revealed that mice injected intraperitoneally with the culture supernatant of the isolate died within 24 h， while all mice in the type E and mixed antitoxin neutralization groups survived， confirming that the isolate produced type E botulinum neurotoxin.ConclusionThis botulism event was caused by bont/E-harboring C. butyricum.
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BibTeX
@article{weiwei2026laboratory,
title = {{Laboratory diagnosis of the first case of Type E botulism caused by Clostridium butyricum in Xinjiang}},
author = {MENG Weiwei and LIN Yujia and WANG Fulong and YUAN Yonghe and SU Jing and HUANG Jia and MA Xin},
journal = {Zhongguo shipin weisheng zazhi},
year = {2026},
month = aug,
doi = {10.13590/j.cjfh.2026.08.004},
url = {https://doi.org/10.13590/j.cjfh.2026.08.004},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Laboratory diagnosis of the first case of Type E botulism caused by Clostridium butyricum in Xinjiang
AU - MENG Weiwei
AU - LIN Yujia
AU - WANG Fulong
AU - YUAN Yonghe
AU - SU Jing
AU - HUANG Jia
AU - MA Xin
PY - 2026
DA - 2026/08/01
JO - Zhongguo shipin weisheng zazhi
DO - 10.13590/j.cjfh.2026.08.004
UR - https://doi.org/10.13590/j.cjfh.2026.08.004
AB - ObjectiveTo conduct laboratory testing on the sample associated with a suspected botulism incident in Xinjiang in February 2025.MethodsThe patient’s fecal specimen was pre-treated in accordance with Chinese health industry standard WS 271-2007. The fecal suspension was inoculated into cooked-meat medium and trypticase peptone glucose yeast extract-trypsin medium （TPGYT）for enrichment culture. Real-time fluorescence quantitative PCR was used to detect botulinum toxin genes in the enrichment broth. Isolation and purification were performed on blood agar， followed by species identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry （MALDI-TOF MS） and whole-genome sequencing. Toxin production by the isolate was confirmed by mouse bioassay.ResultsThe bont/E gene was detected positive by real-time fluorescence PCR in the enriched culture of the patient’s fecal specimen， while type A， B and F bont genes were all negative. One strain with grayish-white， flattened， irregular-edged colonies was isolated on blood agar plates and identified as Clostridium butyricum by MALDI-TOF MS （confidence score 9.542）. Average nucleotide identity （ANI） analysis based on whole-genome sequences showed that this strain shared the highest ANI value of 98.90% with C. butyricum NBRC 13949， with a genome coverage of 90.31%. The bont/E gene carried by the isolate exhibited 100% sequence similarity and 99.92% coverage with the same gene from C. butyricum 5521. The mouse bioassay revealed that mice injected intraperitoneally with the culture supernatant of the isolate died within 24 h， while all mice in the type E and mixed antitoxin neutralization groups survived， confirming that the isolate produced type E botulinum neurotoxin.ConclusionThis botulism event was caused by bont/E-harboring C. butyricum.
SN - 1004-8456
ER -