Research note: Improved virus isolation strategies enhance the detection rate for avian leukosis virus eradication in a local chicken breed in China.
- 1College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, 271018, China.
- 2College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, 271018, China. Electronic address: [email protected].
- 3College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, 271018, China. Electronic address: [email protected].
Abstract
The detection rate of avian leukosis virus subgroup K (ALV-K) has been increasing annually in Chinese local breeds, its poor replicative capacity limits the success of virus isolation. To improve ALV-K eradication, this study assessed the detection limits of the magnetic-particle chemiluminescent assay and ELISA kits using a standardized virus stock. Additionally, we also determined the impact of serial passage during virus isolation on the positive detection rate. Using a standardized ALV-K stock, the magnetic-particle chemiluminescent kit detected positive signals at a 256-fold dilution, whereas the most sensitive ELISA kit reached its limit at a 64-fold dilution. When applied to 13 plasma samples from a local chicken flock, the magnetic-particle chemiluminescent kit at passage 0 yielded a detection rate of 76.9% (10/13), compared to only 15.4% (2/13) by ELISA. After a single cell passage, the chemiluminescent method achieved 100% positivity (13/13), while ELISA required two passages to reach 61.5% (8/13). Quantitative real-time PCR (qPCR) confirmed the presence of ALV-K in at least 9 plasma samples, corroborating the chemiluminescent results. These results demonstrate that the magnetic-particle chemiluminescent assay exhibits significantly higher sensitivity than ELISA and that cell passaging markedly enhances the positive rate of virus isolation. By integrating a highly sensitive chemiluminescent assay with an optimized viral isolation protocol, this study provides a novel strategy for facilitating ALV-K eradication in local Chinese chicken flocks.
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BibTeX
@article{geng2026research,
title = {{Research note: Improved virus isolation strategies enhance the detection rate for avian leukosis virus eradication in a local chicken breed in China.}},
author = {Ruiqiang Geng and Mengyu Song and Wenping Cui and Yixin Wang and Shuang Chang and Zhihao Ren and Peng Zhao},
journal = {Poultry science},
year = {2026},
month = aug,
doi = {10.1016/j.psj.2026.107577},
url = {https://europepmc.org/article/MED/42896422},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Research note: Improved virus isolation strategies enhance the detection rate for avian leukosis virus eradication in a local chicken breed in China.
AU - Ruiqiang Geng
AU - Mengyu Song
AU - Wenping Cui
AU - Yixin Wang
AU - Shuang Chang
AU - Zhihao Ren
AU - Peng Zhao
PY - 2026
DA - 2026/08/05
JO - Poultry science
DO - 10.1016/j.psj.2026.107577
UR - https://europepmc.org/article/MED/42896422
AB - The detection rate of avian leukosis virus subgroup K (ALV-K) has been increasing annually in Chinese local breeds, its poor replicative capacity limits the success of virus isolation. To improve ALV-K eradication, this study assessed the detection limits of the magnetic-particle chemiluminescent assay and ELISA kits using a standardized virus stock. Additionally, we also determined the impact of serial passage during virus isolation on the positive detection rate. Using a standardized ALV-K stock, the magnetic-particle chemiluminescent kit detected positive signals at a 256-fold dilution, whereas the most sensitive ELISA kit reached its limit at a 64-fold dilution. When applied to 13 plasma samples from a local chicken flock, the magnetic-particle chemiluminescent kit at passage 0 yielded a detection rate of 76.9% (10/13), compared to only 15.4% (2/13) by ELISA. After a single cell passage, the chemiluminescent method achieved 100% positivity (13/13), while ELISA required two passages to reach 61.5% (8/13). Quantitative real-time PCR (qPCR) confirmed the presence of ALV-K in at least 9 plasma samples, corroborating the chemiluminescent results. These results demonstrate that the magnetic-particle chemiluminescent assay exhibits significantly higher sensitivity than ELISA and that cell passaging markedly enhances the positive rate of virus isolation. By integrating a highly sensitive chemiluminescent assay with an optimized viral isolation protocol, this study provides a novel strategy for facilitating ALV-K eradication in local Chinese chicken flocks.
SN - 0032-5791
SN - 1525-3171
ER -