Incorporation of Ellagic-Acid-Enriched Lignin into Hydrophilic Electrospun PDLLA/PVP Fibrous Scaffolds Imparts Remarkable Antioxidant and Cellular Redox Protection Properties.
6 affiliations
- 1Department of Chemical Sciences, University of Naples "Federico II", Via Cintia 4, 80126 Naples, Italy.
- 2Department of Basic and Applied Sciences (DISBA), University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.
- 3Department of Theoretical and Applied Sciences (DiSTA), eCampus University, Via Isimbardi 10, 22060 Novedrate, Italy.
- 4Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Viale dell'Università 16, 35020 Legnaro, Italy.
- 5Department of Human Sciences, Link Campus University, Via del Casale di San Pio V 44, 00165 Rome, Italy.
- 6Centro Ricerche per la Chimica Fine Srl for Silvateam Spa, Via Torre 7, 12080 San Michele Mondovì, Italy.
Abstract
A promising strategy to counteract reactive oxygen species (ROS) at cell-material interfaces, a condition that severely compromises cell viability and tissue regeneration, hinges on the incorporation of effective natural antioxidants into biocompatible polymeric scaffolds. Low- and high-molecular-weight phenolic compounds like ellagic acid (EA) and lignin can be easily obtained through green extraction methodologies from wood wastes, such as chestnut wood fiber (CWF), offering biocompatibility and intrinsic redox-protective properties. However, fabricating homogeneous lignin-based biomaterials remains challenging due to the inherent poor solubility properties of lignin. Herein, we report the fabrication of electrospun poly(d,l-lactide)/poly(vinylpyrrolidone) (PDLLA/PVP) mats loaded with an EA-enriched lignin (2% or 4% w/w) extracted from CWF via deep eutectic solvents (DES). The morphological and physicochemical properties of the scaffolds were characterized by scanning electron microscopy, attenuated total reflectance-Fourier transform infrared spectroscopy, and water contact angle. The EA-lignin-enriched mats exhibited remarkable antioxidant capacity, as evaluated by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and cupric reducing antioxidant capacity (CUPRAC) assays, coupled with a sustained and controlled release of EA under physiological conditions. Furthermore, the mats proved highly cytocompatible and capable of significantly mitigating intracellular ROS levels in both normal human dermal fibroblasts and mesenchymal stem cells subjected to oxidative insults. These findings demonstrate that the EA-enriched lignin can be successfully incorporated into electrospun scaffolds, yielding an effective antioxidant biomaterial for biomedical applications.
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BibTeX
@article{argenziano2026incorporation,
title = {{Incorporation of Ellagic-Acid-Enriched Lignin into Hydrophilic Electrospun PDLLA/PVP Fibrous Scaffolds Imparts Remarkable Antioxidant and Cellular Redox Protection Properties.}},
author = {Rita Argenziano and Antonio Laezza and Luca Melotti and Roberta Sacchetto and Brigida Bochicchio and Antonietta Pepe and Samuele Giovando and Lucia Panzella and Alessandra Napolitano},
journal = {Antioxidants (Basel, Switzerland)},
year = {2026},
month = sep,
doi = {10.3390/antiox15101224},
url = {https://europepmc.org/article/MED/42939798},
note = {CC BY},
}
RIS
TY - JOUR
TI - Incorporation of Ellagic-Acid-Enriched Lignin into Hydrophilic Electrospun PDLLA/PVP Fibrous Scaffolds Imparts Remarkable Antioxidant and Cellular Redox Protection Properties.
AU - Rita Argenziano
AU - Antonio Laezza
AU - Luca Melotti
AU - Roberta Sacchetto
AU - Brigida Bochicchio
AU - Antonietta Pepe
AU - Samuele Giovando
AU - Lucia Panzella
AU - Alessandra Napolitano
PY - 2026
DA - 2026/09/22
JO - Antioxidants (Basel, Switzerland)
DO - 10.3390/antiox15101224
UR - https://europepmc.org/article/MED/42939798
AB - A promising strategy to counteract reactive oxygen species (ROS) at cell-material interfaces, a condition that severely compromises cell viability and tissue regeneration, hinges on the incorporation of effective natural antioxidants into biocompatible polymeric scaffolds. Low- and high-molecular-weight phenolic compounds like ellagic acid (EA) and lignin can be easily obtained through green extraction methodologies from wood wastes, such as chestnut wood fiber (CWF), offering biocompatibility and intrinsic redox-protective properties. However, fabricating homogeneous lignin-based biomaterials remains challenging due to the inherent poor solubility properties of lignin. Herein, we report the fabrication of electrospun poly(d,l-lactide)/poly(vinylpyrrolidone) (PDLLA/PVP) mats loaded with an EA-enriched lignin (2% or 4% w/w) extracted from CWF via deep eutectic solvents (DES). The morphological and physicochemical properties of the scaffolds were characterized by scanning electron microscopy, attenuated total reflectance-Fourier transform infrared spectroscopy, and water contact angle. The EA-lignin-enriched mats exhibited remarkable antioxidant capacity, as evaluated by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and cupric reducing antioxidant capacity (CUPRAC) assays, coupled with a sustained and controlled release of EA under physiological conditions. Furthermore, the mats proved highly cytocompatible and capable of significantly mitigating intracellular ROS levels in both normal human dermal fibroblasts and mesenchymal stem cells subjected to oxidative insults. These findings demonstrate that the EA-enriched lignin can be successfully incorporated into electrospun scaffolds, yielding an effective antioxidant biomaterial for biomedical applications.
SN - 2076-3921
ER -