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Peer-reviewedCC BYSTAR protocols

Protocol for standardized minimally invasive mouse models of bisphosphonate-related and radiation-induced jaw osteonecrosis.

  • Zhangfan Ding1
  • Jiayuan Zhang2
  • Hanyu Liu3
  • Abhishek Chandra4
  • Makarand V Risbud5
  • Anjali P Kusumbe6
  • Junyu Chen
6 affiliations
  1. 1State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Tissue and Tumor Microenvironments Lab, Cancer Discovery and Regenerative Medicine Program, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.
  2. 2State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
  3. 3Tissue and Tumor Microenvironments Lab, Cancer Discovery and Regenerative Medicine Program, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.
  4. 4Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA; Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN 55905, USA; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
  5. 5Department of Orthopaedic Surgery, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA; Graduate Program in Cell Biology and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA, USA.
  6. 6Tissue and Tumor Microenvironments Lab, Cancer Discovery and Regenerative Medicine Program, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore; Multidisciplinary Institute of Ageing (MIA-Portugal), University of Coimbra, 3004-504 Coimbra, Portugal. Electronic address: [email protected].
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Abstract

We present a protocol for establishing murine models of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and osteoradionecrosis of the jaw (ORNJ). We describe steps for a uniform, low-trauma tooth extraction technique with disease-specific induction, systemic zoledronic acid for BRONJ and precision-targeted irradiation for ORNJ, to generate robust and clinically relevant models of jaw osteonecrosis, approaching minimizes procedural variability and off-target effects, yielding robust, clinically relevant phenotypes. This protocol provides a standard and minimally invasive framework for investigating jaw osteonecrosis arising from distinct etiologies.

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Cite

BibTeXRIS
BibTeX
@article{ding2026protocol,
  title = {{Protocol for standardized minimally invasive mouse models of bisphosphonate-related and radiation-induced jaw osteonecrosis.}},
  author = {Zhangfan Ding and Jiayuan Zhang and Hanyu Liu and Abhishek Chandra and Makarand V Risbud and Anjali P Kusumbe and Junyu Chen},
  journal = {STAR protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104851},
  url = {https://europepmc.org/article/MED/42832338},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Protocol for standardized minimally invasive mouse models of bisphosphonate-related and radiation-induced jaw osteonecrosis.
AU  - Zhangfan Ding
AU  - Jiayuan Zhang
AU  - Hanyu Liu
AU  - Abhishek Chandra
AU  - Makarand V Risbud
AU  - Anjali P Kusumbe
AU  - Junyu Chen
PY  - 2026
DA  - 2026/10/05
JO  - STAR protocols
DO  - 10.1016/j.xpro.2026.104851
UR  - https://europepmc.org/article/MED/42832338
AB  - We present a protocol for establishing murine models of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and osteoradionecrosis of the jaw (ORNJ). We describe steps for a uniform, low-trauma tooth extraction technique with disease-specific induction, systemic zoledronic acid for BRONJ and precision-targeted irradiation for ORNJ, to generate robust and clinically relevant models of jaw osteonecrosis, approaching minimizes procedural variability and off-target effects, yielding robust, clinically relevant phenotypes. This protocol provides a standard and minimally invasive framework for investigating jaw osteonecrosis arising from distinct etiologies.
SN  - 2666-1667
ER  -