LGALS3BP is associated with selective cargo enrichment in neuroblastoma extracellular vesicles
5 affiliations
- 1Department of Innovative Technologies in Medicine and Dentistry, “G. d’Annunzio” University of Chieti-Pescara
- 2Center for Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara
- 3Department of Neuroscience, Imaging and Clinical Sciences, School of Medicine and Health Sciences, “G. d’Annunzio” University of Chieti-Pescara
- 4Unit of Molecular Genetics, Center for Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara
- 5Department of Science, “G. d’Annunzio” University of Chieti-Pescara
Abstract
Aim: LGALS3BP (also known as Galectin-3-binding protein, Gal-3BP, or 90 K) is a multifunctional secreted glycoprotein involved in tumor progression and has emerged as a potential biomarker and therapeutic target in several malignancies, including neuroblastoma (NB). Increasing evidence suggests that LGALS3BP contributes to shaping the NB tumor microenvironment (TME), particularly through its enrichment on tumor-derived extracellular vesicles (EVs), where it may regulate tumor-stroma interplay. We investigated whether LGALS3BP contributes to stromal cell modulation in NB and is associated with changes in EV cargo composition.
Methods: A patient-derived NB cell line naturally lacking LGALS3BP (hNB CTRL) and a matched counterpart ectopically re-expressing LGALS3BP (hNB LGALS3BP) were used. Bone marrow-derived mesenchymal stem cells (BMMSCs) were exposed to NB-derived conditioned media (CM) to assess cytokine secretion and LGALS3BP detection in recipient cells. EV cargo was analyzed by microRNA (miRNA) and proteomic profiling. Selected candidates were validated by qPCR and immunoblotting on NB-derived EVs.
Results: LGALS3BP-containing CM was associated with an altered BMMSCs secretory profile, while tumor-derived extracellular LGALS3BP was detected in BMMSCs. Seven miRNAs were enriched in LGALS3BP-EVs and reduced in donor cells; four were increased in BMMSCs following EV exposure. Proteomic analysis revealed enrichment of RNA-binding proteins, including SRSF1, in LGALS3BP-EVs.
Conclusions: LGALS3BP is associated with altered EV cargo composition and BMMSCs secretory responses, potentially contributing to tumor-stroma communication within the NB TME.
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BibTeX
@article{cela2026lgals3bp,
title = {{LGALS3BP is associated with selective cargo enrichment in neuroblastoma extracellular vesicles}},
author = {Ilaria Cela and Fani Konstantinidou and Laura Amodei and Arianna Mercatelli and Francesca Piermatteo and Sara Santoro and Asia Pece and Maria Clara Staropoli and Alessia Lamolinara and Emily Capone and Valentina Gatta and Gianluca Sala},
journal = {Frontiers in Oncology},
year = {2026},
month = oct,
doi = {10.3389/fonc.2026.1966526},
url = {https://www.frontiersin.org/articles/10.3389/fonc.2026.1966526/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - LGALS3BP is associated with selective cargo enrichment in neuroblastoma extracellular vesicles
AU - Ilaria Cela
AU - Fani Konstantinidou
AU - Laura Amodei
AU - Arianna Mercatelli
AU - Francesca Piermatteo
AU - Sara Santoro
AU - Asia Pece
AU - Maria Clara Staropoli
AU - Alessia Lamolinara
AU - Emily Capone
AU - Valentina Gatta
AU - Gianluca Sala
PY - 2026
DA - 2026/10/15
JO - Frontiers in Oncology
DO - 10.3389/fonc.2026.1966526
UR - https://www.frontiersin.org/articles/10.3389/fonc.2026.1966526/full
AB - Aim: LGALS3BP (also known as Galectin-3-binding protein, Gal-3BP, or 90 K) is a multifunctional secreted glycoprotein involved in tumor progression and has emerged as a potential biomarker and therapeutic target in several malignancies, including neuroblastoma (NB). Increasing evidence suggests that LGALS3BP contributes to shaping the NB tumor microenvironment (TME), particularly through its enrichment on tumor-derived extracellular vesicles (EVs), where it may regulate tumor-stroma interplay. We investigated whether LGALS3BP contributes to stromal cell modulation in NB and is associated with changes in EV cargo composition. Methods: A patient-derived NB cell line naturally lacking LGALS3BP (hNB CTRL) and a matched counterpart ectopically re-expressing LGALS3BP (hNB LGALS3BP) were used. Bone marrow-derived mesenchymal stem cells (BMMSCs) were exposed to NB-derived conditioned media (CM) to assess cytokine secretion and LGALS3BP detection in recipient cells. EV cargo was analyzed by microRNA (miRNA) and proteomic profiling. Selected candidates were validated by qPCR and immunoblotting on NB-derived EVs. Results: LGALS3BP-containing CM was associated with an altered BMMSCs secretory profile, while tumor-derived extracellular LGALS3BP was detected in BMMSCs. Seven miRNAs were enriched in LGALS3BP-EVs and reduced in donor cells; four were increased in BMMSCs following EV exposure. Proteomic analysis revealed enrichment of RNA-binding proteins, including SRSF1, in LGALS3BP-EVs. Conclusions: LGALS3BP is associated with altered EV cargo composition and BMMSCs secretory responses, potentially contributing to tumor-stroma communication within the NB TME.
SN - 2234-943X
ER -