Screening Central Asian Wheat Germplasm for Powdery Mildew Resistance and Molecular Characterization of Disease Resistance Genes.
- 1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
- 2Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
Abstract
Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a widespread and destructive wheat disease. Employing host powdery mildew (Pm) resistance genes is the most cost-effective and environmentally sustainable approach to managing this disease. In this study, we evaluated seedling resistance in 192 wheat germplasm accessions from three Central Asian countries, Kazakhstan, Kyrgyzstan, and Tajikistan, and identified five accessions with high resistance against Bgt isolate E09. Testing with 12 additional Bgt isolates demonstrated that these five accessions may contain broad-spectrum resistance. Molecular analysis using functional Pm resistance gene markers revealed that two durum wheat accessions, ZY110 and ZY117, may carry Pm68, and one common wheat accession, ZY1, may carry a candidate Pm26 allelic variant. Notably, none of the tested known Pm gene markers were detected in ZY47 and ZY95. Morphological and GISH analysis confirmed that ZY47 and ZY95 possess the rye genome (AABBRR). Molecular marker analysis of three F₂ segregating populations (ZY110 × Kronos, ZY117 × Langdon, and ZY1 × Chuanmai 42) confirmed that each contained a single resistance gene; the diagnostic markers for Pm68 co-segregated with Bgt resistance, while the marker genotypes for Pm26 co-segregated with the expected recessive inheritance model. Sequence alignment confirmed the full-length sequence of Pm68 in durum wheat accessions ZY110 and ZY117, supporting that Pm68 has been used in Central Asian wheat breeding. The candidate Pm26 allelic variant in ZY1, and the candidate sources of novel rye-derived powdery mildew resistance in accessions ZY47 and ZY95, may enrich the powdery mildew resistance gene pool for wheat breeding.
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BibTeX
@article{nosherwan2026screening,
title = {{Screening Central Asian Wheat Germplasm for Powdery Mildew Resistance and Molecular Characterization of Disease Resistance Genes.}},
author = {Muhammad Nosherwan and Muhammad Hammad Latif and Junyu Ma and Tao Chen and Muhammad Saqlain and Qianyi Guo and Ke Zhang and Chunxiao Wang and Wei Zhu and Lili Xu and Dandan Wu and Yonghong Zhou and Houyang Kang and Yinghui Li},
journal = {Plants (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/plants15203106},
url = {https://europepmc.org/article/MED/42943059},
note = {CC BY},
}
RIS
TY - JOUR
TI - Screening Central Asian Wheat Germplasm for Powdery Mildew Resistance and Molecular Characterization of Disease Resistance Genes.
AU - Muhammad Nosherwan
AU - Muhammad Hammad Latif
AU - Junyu Ma
AU - Tao Chen
AU - Muhammad Saqlain
AU - Qianyi Guo
AU - Ke Zhang
AU - Chunxiao Wang
AU - Wei Zhu
AU - Lili Xu
AU - Dandan Wu
AU - Yonghong Zhou
AU - Houyang Kang
AU - Yinghui Li
PY - 2026
DA - 2026/10/10
JO - Plants (Basel, Switzerland)
DO - 10.3390/plants15203106
UR - https://europepmc.org/article/MED/42943059
AB - Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a widespread and destructive wheat disease. Employing host powdery mildew (Pm) resistance genes is the most cost-effective and environmentally sustainable approach to managing this disease. In this study, we evaluated seedling resistance in 192 wheat germplasm accessions from three Central Asian countries, Kazakhstan, Kyrgyzstan, and Tajikistan, and identified five accessions with high resistance against Bgt isolate E09. Testing with 12 additional Bgt isolates demonstrated that these five accessions may contain broad-spectrum resistance. Molecular analysis using functional Pm resistance gene markers revealed that two durum wheat accessions, ZY110 and ZY117, may carry Pm68, and one common wheat accession, ZY1, may carry a candidate Pm26 allelic variant. Notably, none of the tested known Pm gene markers were detected in ZY47 and ZY95. Morphological and GISH analysis confirmed that ZY47 and ZY95 possess the rye genome (AABBRR). Molecular marker analysis of three F₂ segregating populations (ZY110 × Kronos, ZY117 × Langdon, and ZY1 × Chuanmai 42) confirmed that each contained a single resistance gene; the diagnostic markers for Pm68 co-segregated with Bgt resistance, while the marker genotypes for Pm26 co-segregated with the expected recessive inheritance model. Sequence alignment confirmed the full-length sequence of Pm68 in durum wheat accessions ZY110 and ZY117, supporting that Pm68 has been used in Central Asian wheat breeding. The candidate Pm26 allelic variant in ZY1, and the candidate sources of novel rye-derived powdery mildew resistance in accessions ZY47 and ZY95, may enrich the powdery mildew resistance gene pool for wheat breeding.
SN - 2223-7747
ER -