Production, Optimization and Characterization of Biobased Itaconic Acid from Saccharomyces cerevisiae
4 affiliations
- 1Science and Technology Application and Research Center, Dokuz Eylul University, 35390 Buca, Türkiye
- 2Biochemistry Division, Department of Chemistry, Faculty of Science, Dokuz Eylul University, 35390 Buca, Türkiye
- 3Biotechnology Department, The Graduate School of Natural and Applied Sciences, Dokuz Eylul University, 35390 Buca, Türkiye
- 4Leather Engineering Department, Faculty of Engineering, Ege University, 35100 Bornova, Türkiye
Abstract
Itaconic acid (IA) is an important bio-based platform chemical with applications in the production of polymers, coatings, resins, and other value-added materials. However, its conventional microbial production relies predominantly on microorganisms that present safety and process-related limitations. This study investigated Saccharomyces cerevisiae BY4742 as a potentially safer microbial platform for the production of bio-based IA under submerged fermentation conditions. IA production was optimized using response surface methodology with a Box–Behnken design, considering glucose concentration, initial pH, and CaCI2 concentration as independent variables. The maximum IA concentration of 1133.50 ± 54.92 mg/L was obtained at 30 °C, pH 4.5, with 10% (w/v) glucose and without CaCI2 supplementation after 216 h of fermentation. The fermentation-derived IA was subsequently recovered and characterized using high-performance liquid chromatography, Fourier-transform infrared spectroscopy, 1H-nuclear magnetic resonance (1H-NMR), and thermogravimetric analysis. The analytical results confirmed the molecular identity and high chromatographic purity of the recovered IA. Furthermore, the homopolymerization ability of the fermentation-derived IA was evaluated and compared with that of commercial IA under the same reaction conditions. The 1H-NMR results demonstrated changes consistent with consumption of the IA C=C double bond following polymerization, indicating that the fermentation-derived IA retained its ability to undergo homopolymerization. Overall, these findings demonstrate the potential of S. cerevisiae for producing structurally confirmed bio-based IA and provide a proof of concept for its use as a renewable monomer for poly(itaconic acid) synthesis, supporting its further investigation for the development of bio-based polymeric materials.
Related
- Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae
- Differentiation of Indigenous Saccharomyces cerevisiae Wine Strains by Oenological and Proteomic Characterization
- Scp160 deletion suppresses TDP-43 aggregation and toxicity in Saccharomyces cerevisiae
- Chemogenomic profiling of diverse Saccharomyces cerevisiae strains using the novel CRISPR-Cas9 markerless barcoded library BarMix
- The Linker Histone Plays a Dual Role during Gametogenesis in Saccharomyces cerevisiae
BibTeX
@article{tosuner2026production,
title = {{Production, Optimization and Characterization of Biobased Itaconic Acid from Saccharomyces cerevisiae}},
author = {Zulfiye Velioglu Tosuner and Mehdi Kosaca and Raziye Ozturk Urek and Onur Yilmaz and Catalina Natalia Yilmaz},
journal = {International journal of molecular sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27198923},
url = {https://europepmc.org/article/PMC/PMC13666190},
note = {CC BY},
}
RIS
TY - JOUR
TI - Production, Optimization and Characterization of Biobased Itaconic Acid from Saccharomyces cerevisiae
AU - Zulfiye Velioglu Tosuner
AU - Mehdi Kosaca
AU - Raziye Ozturk Urek
AU - Onur Yilmaz
AU - Catalina Natalia Yilmaz
PY - 2026
DA - 2026/10/01
JO - International journal of molecular sciences
DO - 10.3390/ijms27198923
UR - https://europepmc.org/article/PMC/PMC13666190
AB - Itaconic acid (IA) is an important bio-based platform chemical with applications in the production of polymers, coatings, resins, and other value-added materials. However, its conventional microbial production relies predominantly on microorganisms that present safety and process-related limitations. This study investigated Saccharomyces cerevisiae BY4742 as a potentially safer microbial platform for the production of bio-based IA under submerged fermentation conditions. IA production was optimized using response surface methodology with a Box–Behnken design, considering glucose concentration, initial pH, and CaCI2 concentration as independent variables. The maximum IA concentration of 1133.50 ± 54.92 mg/L was obtained at 30 °C, pH 4.5, with 10% (w/v) glucose and without CaCI2 supplementation after 216 h of fermentation. The fermentation-derived IA was subsequently recovered and characterized using high-performance liquid chromatography, Fourier-transform infrared spectroscopy, 1H-nuclear magnetic resonance (1H-NMR), and thermogravimetric analysis. The analytical results confirmed the molecular identity and high chromatographic purity of the recovered IA. Furthermore, the homopolymerization ability of the fermentation-derived IA was evaluated and compared with that of commercial IA under the same reaction conditions. The 1H-NMR results demonstrated changes consistent with consumption of the IA C=C double bond following polymerization, indicating that the fermentation-derived IA retained its ability to undergo homopolymerization. Overall, these findings demonstrate the potential of S. cerevisiae for producing structurally confirmed bio-based IA and provide a proof of concept for its use as a renewable monomer for poly(itaconic acid) synthesis, supporting its further investigation for the development of bio-based polymeric materials.
SN - 1422-0067
ER -