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PreprintCC BY-NCbioRxiv

CytosMPS: an automated multimodal pooled screening platform for protein, morphology and transcriptomics

  • T. Lopez
  • J. Rabalais
  • B. Song
  • A. Boddicker
  • A. Schwartz
  • D. Honigfort
  • J. Zhao
  • M. Kellinger
  • S. Kruglyak1
  1. 1Element Biosciences, San Diego, CA, USA
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Abstract

Cytos Multimodal Pooled Screening (Cytos MPS) measures CRISPR guide identity, morphology, native 3' transcriptome, and protein abundance and localization in the same fixed cells. Following IL-1{beta} stimulation, cells with higher nuclear NF-{kappa}B show lower phospho-p38/HSP27 and higher NFKBIA/DUSP1 in the same cell, a measurement neither imaging nor transcriptomics alone provides. TGFBR2 and CTNNB1 perturbations produce distinct multimodal phenotypes. Guide representation and perturbation transcriptional responses agree with an orthogonal single-cell transcriptomic platform.

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Cite

BibTeXRIS
BibTeX
@misc{lopez2026cytosmps,
  title = {{CytosMPS: an automated multimodal pooled screening platform for protein, morphology and transcriptomics}},
  author = {T. Lopez and J. Rabalais and B. Song and A. Boddicker and A. Schwartz and D. Honigfort and J. Zhao and M. Kellinger and S. Kruglyak},
  howpublished = {bioRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.20.752925},
  url = {https://www.biorxiv.org/content/10.64898/2026.09.20.752925v1},
  note = {CC BY-NC},
}
RIS
TY  - UNPB
TI  - CytosMPS: an automated multimodal pooled screening platform for protein, morphology and transcriptomics
AU  - T. Lopez
AU  - J. Rabalais
AU  - B. Song
AU  - A. Boddicker
AU  - A. Schwartz
AU  - D. Honigfort
AU  - J. Zhao
AU  - M. Kellinger
AU  - S. Kruglyak
PY  - 2026
DA  - 2026/09/20
PB  - bioRxiv
DO  - 10.64898/2026.09.20.752925
UR  - https://www.biorxiv.org/content/10.64898/2026.09.20.752925v1
AB  - Cytos Multimodal Pooled Screening (Cytos MPS) measures CRISPR guide identity, morphology, native 3' transcriptome, and protein abundance and localization in the same fixed cells. Following IL-1{beta} stimulation, cells with higher nuclear NF-{kappa}B show lower phospho-p38/HSP27 and higher NFKBIA/DUSP1 in the same cell, a measurement neither imaging nor transcriptomics alone provides. TGFBR2 and CTNNB1 perturbations produce distinct multimodal phenotypes. Guide representation and perturbation transcriptional responses agree with an orthogonal single-cell transcriptomic platform.
ER  -