Identification of the TLP gene family in Rosaceae and function of PbrTLP28 in leaf disease resistance
- 1Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-construction by Ministry and Province), Anhui Provincial Key Laboratory for Germplasm Resources Creation and High-Efficiency Cultivation of Horticultural Crops, Institute of Horticulture, Anhui Academy of Agricultural Sciences
- 2Sanya Institute of Nanjing Agricultural University, National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University
Abstract
Thaumatin-like proteins (TLPs) are induced by various biotic and abiotic signals, exhibit antifungal activity, and serve essential functions in plant development and stress defense. However, studies on the identification of TLPs in Rosaceae fruit trees and their specific functions in leaf disease resistance remain limited. In this study, 177 TLPs were characterized from five Rosaceae genomes ( Fragaria vesca , Malus domestica , Pyrus bretschneideri , Prunus mume , and Prunus persica ) and classified into 11 subfamilies (Groups 1-11) according to their phylogenetic and structural features. Diverse gene duplication modes facilitated the expansion of the TLP family, whereas purifying selection served as the primary driving force of TLP evolution. Notably, expression analysis revealed that PbrTLP28 was significantly upregulated following black spot disease infection. Signal peptide prediction, conserved domain analysis, and subcellular localization assays confirmed that PbrTLP28 is a secreted protein. Functional assays demonstrated that silencing PbrTLP28 attenuated the resistance of pear leaves to black spot disease, indicating that PbrTLP28 functions in disease resistance. Collectively, these findings provide significant insights into TLP gene function, expression, and evolution in Rosaceae.
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BibTeX
@article{zhang2026identification,
title = {{Identification of the TLP gene family in Rosaceae and function of PbrTLP28 in leaf disease resistance}},
author = {Hao Zhang and Xueying Liu and Ruobing Sun and Huishu Yan and Chi Lan and Ziwen Xiao and Na Ma and Liqing Lu and Haiqi Zhang and Chunyan Liu and Peng Wang and Shaoling Zhang and Juyou Wu and Yongjie Qi and Zhenghui Gao},
journal = {Frontiers in Plant Science},
year = {2026},
month = oct,
doi = {10.3389/fpls.2026.1990307},
url = {https://www.frontiersin.org/articles/10.3389/fpls.2026.1990307/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Identification of the TLP gene family in Rosaceae and function of PbrTLP28 in leaf disease resistance
AU - Hao Zhang
AU - Xueying Liu
AU - Ruobing Sun
AU - Huishu Yan
AU - Chi Lan
AU - Ziwen Xiao
AU - Na Ma
AU - Liqing Lu
AU - Haiqi Zhang
AU - Chunyan Liu
AU - Peng Wang
AU - Shaoling Zhang
AU - Juyou Wu
AU - Yongjie Qi
AU - Zhenghui Gao
PY - 2026
DA - 2026/10/28
JO - Frontiers in Plant Science
DO - 10.3389/fpls.2026.1990307
UR - https://www.frontiersin.org/articles/10.3389/fpls.2026.1990307/full
AB - Thaumatin-like proteins (TLPs) are induced by various biotic and abiotic signals, exhibit antifungal activity, and serve essential functions in plant development and stress defense. However, studies on the identification of TLPs in Rosaceae fruit trees and their specific functions in leaf disease resistance remain limited. In this study, 177 TLPs were characterized from five Rosaceae genomes ( Fragaria vesca , Malus domestica , Pyrus bretschneideri , Prunus mume , and Prunus persica ) and classified into 11 subfamilies (Groups 1-11) according to their phylogenetic and structural features. Diverse gene duplication modes facilitated the expansion of the TLP family, whereas purifying selection served as the primary driving force of TLP evolution. Notably, expression analysis revealed that PbrTLP28 was significantly upregulated following black spot disease infection. Signal peptide prediction, conserved domain analysis, and subcellular localization assays confirmed that PbrTLP28 is a secreted protein. Functional assays demonstrated that silencing PbrTLP28 attenuated the resistance of pear leaves to black spot disease, indicating that PbrTLP28 functions in disease resistance. Collectively, these findings provide significant insights into TLP gene function, expression, and evolution in Rosaceae.
SN - 1664-462X
ER -