Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics
Abstract
Advancements in plant genome sequencing and assembly have enabled the production of increasingly accurate and contiguous genome sequences. Here, we present the chromosome‐level assembly of the durum wheat ( Triticum turgidum L. ssp. durum , cv. Svevo) reference genome produced using accurate long‐reads, optical mapping and Hi‐C. The new assembly (Svevo Rel.2.0) comprises 263 hybrid scaffolds with an N50 value of 112.3 Mb, arranged into 14 contiguous pseudomolecules spanning 10.4 Gb. The Svevo Rel.2.0 genome assembly was annotated using extensive short‐ and long‐read RNA sequencing data obtained from 60 tissue/treatment combinations. The resulting annotation comprises 68 154 high‐confidence protein‐coding genes, which have been integrated into a comprehensive transcriptome atlas accessible through an eFP browser. Annotation was manually curated for storage protein gene families and for Leucine‐Rich Repeat‐Containing Receptor genes yielding 3763 LRR‐CR loci. The genome assembly's accuracy and completeness were demonstrated by the correct reconstruction of the physical map of Tg1‐B ( Tenacious glumes 1 ), a locus controlling the free threshing trait located on chromosome 2B that was not assembled in the previous genome release (Svevo Rel.1.0). A wealth of 6621 QTLs/MTAs from the literature were mapped onto Svevo Rel.2.0 to identify QTL hotspots and trait‐specific candidate genes. The ancestry of the durum genome to representative wild emmer populations from North‐Eastern and Southern‐Levant Fertile Crescent assessed by tracing haplotype transmission patterns revealed a clear mosaic pattern. This new durum reference genome, enhanced with advanced annotation and an expression atlas linked to QTLome data, is the most comprehensive tool available for durum wheat genomics.
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BibTeX
@article{mazzucotelli2026durum,
title = {{Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics}},
author = {Elisabetta Mazzucotelli and Cristian Forestan and Gina Zastrow-Hayes and David Swarbreck and Yael Lev-Mirom and Emile Cavalet-Giorsa and Matteo Bozzoli and Anna Maria Mastrangelo and Daniela Marone and Vincent Ranwez and Johanna Girodolle and Victor Llaca and Kevin Fengler and Charlotte Harris and Helena Toegelová and Pavla Navrátilová and Primetta Faccioli and Francesca Desiderio and Ana Paola Valladares and Gemy Kaithakottil and Rachel L. Rusholme-Pilcher and Manuel Spannagl and Heidrun Gundlach and Klaus F. X. Mayer and Victoria C. Blake and Justin D. Faris and Steven S. Xu and Taner Z. Sen and Eric Yao and Julio Isidro Y Sánchez and Chunyi Liu and Muhammad A Farooq and Sandra Stefanelli and Chiara Cappucci and Leah Oren and Tamar Eilam and Mario Giorgioni and Alessandra Stella and Barbara Lazzari and Massimiliano Lauria and Aldo Ceriotti and Raul Pirona and Elahe Tavakol and Valentyna Klymiuk and Jennifer Ens and Harmeet Singh Chawla and Sean Walkowiak and Nicola Pecchioni and Filippo M. Bassi and Miguel Sanchez-Garcia and Gabriella Sonnante and Pasquale L. Curci and Giovanni Giuliano and Giuseppe Aprea and Asher Pasha and Agata Gadaleta and Ilaria Marcotuli and Stefania Masci and Francesco Sestili and Samuela Palombieri},
journal = {Plant Biotechnology Journal},
year = {2026},
month = jul,
doi = {10.1111/pbi.70673},
url = {https://hal.inrae.fr/hal-05762080v2},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics
AU - Elisabetta Mazzucotelli
AU - Cristian Forestan
AU - Gina Zastrow-Hayes
AU - David Swarbreck
AU - Yael Lev-Mirom
AU - Emile Cavalet-Giorsa
AU - Matteo Bozzoli
AU - Anna Maria Mastrangelo
AU - Daniela Marone
AU - Vincent Ranwez
AU - Johanna Girodolle
AU - Victor Llaca
AU - Kevin Fengler
AU - Charlotte Harris
AU - Helena Toegelová
AU - Pavla Navrátilová
AU - Primetta Faccioli
AU - Francesca Desiderio
AU - Ana Paola Valladares
AU - Gemy Kaithakottil
AU - Rachel L. Rusholme-Pilcher
AU - Manuel Spannagl
AU - Heidrun Gundlach
AU - Klaus F. X. Mayer
AU - Victoria C. Blake
AU - Justin D. Faris
AU - Steven S. Xu
AU - Taner Z. Sen
AU - Eric Yao
AU - Julio Isidro Y Sánchez
AU - Chunyi Liu
AU - Muhammad A Farooq
AU - Sandra Stefanelli
AU - Chiara Cappucci
AU - Leah Oren
AU - Tamar Eilam
AU - Mario Giorgioni
AU - Alessandra Stella
AU - Barbara Lazzari
AU - Massimiliano Lauria
AU - Aldo Ceriotti
AU - Raul Pirona
AU - Elahe Tavakol
AU - Valentyna Klymiuk
AU - Jennifer Ens
AU - Harmeet Singh Chawla
AU - Sean Walkowiak
AU - Nicola Pecchioni
AU - Filippo M. Bassi
AU - Miguel Sanchez-Garcia
AU - Gabriella Sonnante
AU - Pasquale L. Curci
AU - Giovanni Giuliano
AU - Giuseppe Aprea
AU - Asher Pasha
AU - Agata Gadaleta
AU - Ilaria Marcotuli
AU - Stefania Masci
AU - Francesco Sestili
AU - Samuela Palombieri
PY - 2026
DA - 2026/07/13
JO - Plant Biotechnology Journal
DO - 10.1111/pbi.70673
UR - https://hal.inrae.fr/hal-05762080v2
AB - Advancements in plant genome sequencing and assembly have enabled the production of increasingly accurate and contiguous genome sequences. Here, we present the chromosome‐level assembly of the durum wheat ( Triticum turgidum L. ssp. durum , cv. Svevo) reference genome produced using accurate long‐reads, optical mapping and Hi‐C. The new assembly (Svevo Rel.2.0) comprises 263 hybrid scaffolds with an N50 value of 112.3 Mb, arranged into 14 contiguous pseudomolecules spanning 10.4 Gb. The Svevo Rel.2.0 genome assembly was annotated using extensive short‐ and long‐read RNA sequencing data obtained from 60 tissue/treatment combinations. The resulting annotation comprises 68 154 high‐confidence protein‐coding genes, which have been integrated into a comprehensive transcriptome atlas accessible through an eFP browser. Annotation was manually curated for storage protein gene families and for Leucine‐Rich Repeat‐Containing Receptor genes yielding 3763 LRR‐CR loci. The genome assembly's accuracy and completeness were demonstrated by the correct reconstruction of the physical map of Tg1‐B ( Tenacious glumes 1 ), a locus controlling the free threshing trait located on chromosome 2B that was not assembled in the previous genome release (Svevo Rel.1.0). A wealth of 6621 QTLs/MTAs from the literature were mapped onto Svevo Rel.2.0 to identify QTL hotspots and trait‐specific candidate genes. The ancestry of the durum genome to representative wild emmer populations from North‐Eastern and Southern‐Levant Fertile Crescent assessed by tracing haplotype transmission patterns revealed a clear mosaic pattern. This new durum reference genome, enhanced with advanced annotation and an expression atlas linked to QTLome data, is the most comprehensive tool available for durum wheat genomics.
SN - 1467-7644
SN - 1467-7652
ER -