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Peer-reviewedCC BYSTAR protocols

Agarose-free protocol for preparing human organotypic lung cultures to study respiratory virus infection and evaluate antivirals ex vivo.

  • Lola Canus1
  • Florentine Jacolin2
  • Virginie Vasseur3
  • Adeline Cezard3
  • Eva Ogire1
  • Anne Aublin-Gex2
  • Amelie Bourgeais1
  • Camille David3
  • Alexandra Erny4
  • Fabienne Archer4
  • Antoine Legras5
  • Damien Sizaret6
6 more authors
  • Antoine Guillon7
  • Vincent Lotteau8
  • Pierre-Olivier Vidalain2
  • Mustapha Si-Tahar3
  • Laure Perrin-Cocon2
  • Cyrille Mathieu9
9 affiliations
  1. 1CIRI, Centre International de Recherche en Infectiologie, Team NeuroInvasion, Tropism and Viral Encephalitis, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
  2. 2CIRI, Centre International de Recherche en Infectiologie, Team Viral Infection, Metabolism and Immunity, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
  3. 3Inserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France.
  4. 4IVPC, Infections Virales et Pathologie Comparée, UMR754, INRAE, Université Lyon 1, EPHE, 69007 Lyon, France.
  5. 5Service de Chirurgie Thoracique, CHRU de Tours, Université de Tours, Tours, France.
  6. 6Service de Pathologie, CHRU de Tours, Tours, France.
  7. 7Inserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France; Service de Médecine Intensive -Réanimation, CHRU de Tours, Tours, France.
  8. 8Laboratoire P4 INSERM-Jean Mérieux, Lyon, France.
  9. 9CIRI, Centre International de Recherche en Infectiologie, Team NeuroInvasion, Tropism and Viral Encephalitis, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France. Electronic address: [email protected].
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Abstract

Here, we present a protocol to generate human organotypic lung cultures from surgical lung resection for the study of respiratory infections. We describe steps for tissue harvesting, biopsy punching, mechanical slicing, and culture at the air-liquid interface. This technique enables robust ex vivo infections of human lung tissues with respiratory viruses, including influenza A and Nipah. It can be used to study host-pathogen interactions, analyze innate immune responses, and evaluate antiviral candidates in physiologically relevant human lung tissue. For complete details on the use and execution of this protocol, please refer to Cezard et al.¹.

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Cite

BibTeXRIS
BibTeX
@article{canus2026agarosefree,
  title = {{Agarose-free protocol for preparing human organotypic lung cultures to study respiratory virus infection and evaluate antivirals ex vivo.}},
  author = {Lola Canus and Florentine Jacolin and Virginie Vasseur and Adeline Cezard and Eva Ogire and Anne Aublin-Gex and Amelie Bourgeais and Camille David and Alexandra Erny and Fabienne Archer and Antoine Legras and Damien Sizaret and Antoine Guillon and Vincent Lotteau and Pierre-Olivier Vidalain and Mustapha Si-Tahar and Laure Perrin-Cocon and Cyrille Mathieu},
  journal = {STAR protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104896},
  url = {https://europepmc.org/article/MED/42873646},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Agarose-free protocol for preparing human organotypic lung cultures to study respiratory virus infection and evaluate antivirals ex vivo.
AU  - Lola Canus
AU  - Florentine Jacolin
AU  - Virginie Vasseur
AU  - Adeline Cezard
AU  - Eva Ogire
AU  - Anne Aublin-Gex
AU  - Amelie Bourgeais
AU  - Camille David
AU  - Alexandra Erny
AU  - Fabienne Archer
AU  - Antoine Legras
AU  - Damien Sizaret
AU  - Antoine Guillon
AU  - Vincent Lotteau
AU  - Pierre-Olivier Vidalain
AU  - Mustapha Si-Tahar
AU  - Laure Perrin-Cocon
AU  - Cyrille Mathieu
PY  - 2026
DA  - 2026/10/13
JO  - STAR protocols
DO  - 10.1016/j.xpro.2026.104896
UR  - https://europepmc.org/article/MED/42873646
AB  - Here, we present a protocol to generate human organotypic lung cultures from surgical lung resection for the study of respiratory infections. We describe steps for tissue harvesting, biopsy punching, mechanical slicing, and culture at the air-liquid interface. This technique enables robust ex vivo infections of human lung tissues with respiratory viruses, including influenza A and Nipah. It can be used to study host-pathogen interactions, analyze innate immune responses, and evaluate antiviral candidates in physiologically relevant human lung tissue. For complete details on the use and execution of this protocol, please refer to Cezard et al.¹.
SN  - 2666-1667
ER  -