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Peer-reviewedCC BYVaccines

Epitope Mapping and MHC Restriction of Next-Generation Tuberculosis Vaccine Antigens in Mice.

  • Samuel J Vidal1
  • Alejandro A Espinosa Perez1
  • Ganad D Neka1
  • Aleisha M G Roberts1
  • Yu Sun1
  • Lili Benitez Angeles1
  • Hannah I Angulo Espinoza1
  • Ellis A Strutton1
  • Esther R Mbiwan1
  • Jayeshbhai Chaudhari1
  • Woojae Choi1
  • Michelle Lifton1
2 more authors
  • Pablo Penaloza-MacMaster1
  • Dan H Barouch1
  1. 1Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
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Abstract

Background/Objectives: Classical major histocompatibility complex (MHC)-restricted T cells play essential roles in tuberculosis (TB) immunity. Characterizing the antigen targets, dominant epitopes, and MHC restriction of protective T-cell responses is important for understanding TB immunity and next-generation TB vaccine development. Methods: We recently used vaccine screening in mice to identify Rv1387 (PPE20), Rv0287 (EsxG), Rv1788 (PE18), and Rv1886c (Ag85B) as protective antigens, and in this paper, we characterized the T-cell responses to these antigens in mice. Results: We first identified the full repertoire of CD4 and CD8 T-cell epitopes in C57BL/6 and BALB/c mice. We further defined the MHC class I and II restriction of immunodominant epitopes, enabling the development and validation of tetramers for probing cognate T-cell responses. Finally, we used these tetramers to contrast lung mucosal T-cell phenotypes in the setting of Bacillus Calmette-Guérin (BCG) and mRNA vaccination. Conclusions: These results define the T-cell epitopes and MHC restriction of next-generation TB vaccine antigens and contribute reagents for studying TB T-cell immunity.

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Cite

BibTeXRIS
BibTeX
@article{vidal2026epitope,
  title = {{Epitope Mapping and MHC Restriction of Next-Generation Tuberculosis Vaccine Antigens in Mice.}},
  author = {Samuel J Vidal and Alejandro A Espinosa Perez and Ganad D Neka and Aleisha M G Roberts and Yu Sun and Lili Benitez Angeles and Hannah I Angulo Espinoza and Ellis A Strutton and Esther R Mbiwan and Jayeshbhai Chaudhari and Woojae Choi and Michelle Lifton and Pablo Penaloza-MacMaster and Dan H Barouch},
  journal = {Vaccines},
  year = {2026},
  month = oct,
  doi = {10.3390/vaccines14100866},
  url = {https://europepmc.org/article/MED/42931395},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Epitope Mapping and MHC Restriction of Next-Generation Tuberculosis Vaccine Antigens in Mice.
AU  - Samuel J Vidal
AU  - Alejandro A Espinosa Perez
AU  - Ganad D Neka
AU  - Aleisha M G Roberts
AU  - Yu Sun
AU  - Lili Benitez Angeles
AU  - Hannah I Angulo Espinoza
AU  - Ellis A Strutton
AU  - Esther R Mbiwan
AU  - Jayeshbhai Chaudhari
AU  - Woojae Choi
AU  - Michelle Lifton
AU  - Pablo Penaloza-MacMaster
AU  - Dan H Barouch
PY  - 2026
DA  - 2026/10/01
JO  - Vaccines
DO  - 10.3390/vaccines14100866
UR  - https://europepmc.org/article/MED/42931395
AB  - Background/Objectives: Classical major histocompatibility complex (MHC)-restricted T cells play essential roles in tuberculosis (TB) immunity. Characterizing the antigen targets, dominant epitopes, and MHC restriction of protective T-cell responses is important for understanding TB immunity and next-generation TB vaccine development. Methods: We recently used vaccine screening in mice to identify Rv1387 (PPE20), Rv0287 (EsxG), Rv1788 (PE18), and Rv1886c (Ag85B) as protective antigens, and in this paper, we characterized the T-cell responses to these antigens in mice. Results: We first identified the full repertoire of CD4 and CD8 T-cell epitopes in C57BL/6 and BALB/c mice. We further defined the MHC class I and II restriction of immunodominant epitopes, enabling the development and validation of tetramers for probing cognate T-cell responses. Finally, we used these tetramers to contrast lung mucosal T-cell phenotypes in the setting of Bacillus Calmette-Guérin (BCG) and mRNA vaccination. Conclusions: These results define the T-cell epitopes and MHC restriction of next-generation TB vaccine antigens and contribute reagents for studying TB T-cell immunity.
SN  - 2076-393X
ER  -