Natural variation in the promoter of OsPDX1.2 enhances vitamin B6 accumulation and cold tolerance in rice
6 affiliations
- 1School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication) Hainan University Sanya 572025 China
- 2Yazhouwan National Laboratory Sanya 572025 China
- 3Hainan Seed Industry Laboratory Sanya 572025 China
- 4Sanya Institute Hainan Academy of Agricultural Sciences Sanya 572025 China
- 5Max Planck Institute of Molecular Plant Physiology Potsdam‐Golm 14476 Germany
- 6Henan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy Henan Agricultural University Zhengzhou 450046 China
Abstract
Vitamin B6 (VB6) is an essential nutrient that must be obtained from the diet by humans and livestock, and plays an important role in response to various biotic and abiotic stresses in plants. Although the VB6 biosynthesis pathway has been uncovered in higher plants, understanding of its genetic control remains limited, and the role of VB6 in responding to cold stress is largely unknown. Here, we showed that variation in VB6 levels in rice is attributable to differential transcription of OsPDX1.2, a pyridoxal phosphate synthase, due to polymorphisms in its promoter. Natural variations in the OsPDX1.2 promoter affect the binding affinity of OsbZIP18, a transcriptional factor repressing OsPDX1.2 transcription. Loss of function in OsPDX1.2 or overexpression of OsbZIP18 significantly reduces cold tolerance in rice. Further investigation revealed that both genes affect cold tolerance by regulating VB6 and abscisic acid (ABA) biosynthesis. Moreover, the frequency of the cold‐tolerant pOsPDX1.2
Hap2 increases at higher latitudes, whereas the cold‐sensitive pOsPDX1.2
Hap1 increases at lower latitudes, indicating that natural variation in OsPDX1.2 contributes to differential cold adaptation in rice. Overall, our results reveal a regulatory pathway controlling rice VB6 accumulation and cold tolerance via the OsbZIP18–OsPDX1.2 module, providing a potential target for improving rice quality and stress tolerance.
Related
- Exogenous melatonin enhances drought tolerance and secondary-metabolite accumulation in Paeonia ostii 'Fengdan' in association with improved PSI-PSII photochemical coordination.
- Carboxymethyl cellulose-ferulic acid nanocomposite enhances salinity tolerance and phytochemical accumulation in Dracocephalum moldavica via coordinated redox and osmotic regulation.
- Arabidopsis thaliana root responses to Cd exposure: insights into root tip‐specific changes and the role of HY5 in limiting Cd accumulation and promoting tolerance
- Loss of ALN Function in Arabidopsis thaliana Induces Allantoin Accumulation and Enhances Antioxidant Enzyme Responses to Copper Toxicity
- Heterologous Expression of Saussurea involucrata SiPIP1;5b Enhances Freezing Tolerance in Arabidopsis and Chilling Tolerance in Tomato
BibTeX
@article{wang2026natural,
title = {{Natural variation in the promoter of OsPDX1.2 enhances vitamin B6 accumulation and cold tolerance in rice}},
author = {Bi Wang and Qiu Yi Wang and Wen Jie Shi and Rong Hui Li and Xu Dong Zhang and Li Ping Wang and Shi Jia Xia and Kai Xi Chen and Jun Jie Zhou and Yang Yang Sun and Jing He and Alisdair R. Fernie and Ying Chun Han and Jun Zhou Li and Yu Heng Shi and Xian Qing Liu and Yuan Yuan Zhang and Jie Luo and Cheng Jin},
journal = {Journal of Integrative Plant Biology},
year = {2026},
month = jun,
doi = {10.1111/jipb.70322},
url = {https://doi.org/10.1111/jipb.70322},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Natural variation in the promoter of OsPDX1.2 enhances vitamin B6 accumulation and cold tolerance in rice
AU - Bi Wang
AU - Qiu Yi Wang
AU - Wen Jie Shi
AU - Rong Hui Li
AU - Xu Dong Zhang
AU - Li Ping Wang
AU - Shi Jia Xia
AU - Kai Xi Chen
AU - Jun Jie Zhou
AU - Yang Yang Sun
AU - Jing He
AU - Alisdair R. Fernie
AU - Ying Chun Han
AU - Jun Zhou Li
AU - Yu Heng Shi
AU - Xian Qing Liu
AU - Yuan Yuan Zhang
AU - Jie Luo
AU - Cheng Jin
PY - 2026
DA - 2026/06/15
JO - Journal of Integrative Plant Biology
DO - 10.1111/jipb.70322
UR - https://doi.org/10.1111/jipb.70322
AB - Vitamin B6 (VB6) is an essential nutrient that must be obtained from the diet by humans and livestock, and plays an important role in response to various biotic and abiotic stresses in plants. Although the VB6 biosynthesis pathway has been uncovered in higher plants, understanding of its genetic control remains limited, and the role of VB6 in responding to cold stress is largely unknown. Here, we showed that variation in VB6 levels in rice is attributable to differential transcription of OsPDX1.2, a pyridoxal phosphate synthase, due to polymorphisms in its promoter. Natural variations in the OsPDX1.2 promoter affect the binding affinity of OsbZIP18, a transcriptional factor repressing OsPDX1.2 transcription. Loss of function in OsPDX1.2 or overexpression of OsbZIP18 significantly reduces cold tolerance in rice. Further investigation revealed that both genes affect cold tolerance by regulating VB6 and abscisic acid (ABA) biosynthesis. Moreover, the frequency of the cold‐tolerant pOsPDX1.2 Hap2 increases at higher latitudes, whereas the cold‐sensitive pOsPDX1.2 Hap1 increases at lower latitudes, indicating that natural variation in OsPDX1.2 contributes to differential cold adaptation in rice. Overall, our results reveal a regulatory pathway controlling rice VB6 accumulation and cold tolerance via the OsbZIP18–OsPDX1.2 module, providing a potential target for improving rice quality and stress tolerance.
SN - 1672-9072
SN - 1744-7909
ER -