Integrated Multi-Omics Analysis of the Response of Lycium barbarum ‘Ningqi-1’ to Powdery Mildew Infection and Functional Characterization of LbMLO6
- 1School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China
- 2National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
- 3National Wolfberry Engineering Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China
Abstract
Powdery mildew severely constrains the production of Lycium barbarum L., yet the molecular mechanisms underlying its response to infection remain poorly understood. Here, ‘Ningqi-1’ plants were inoculated with the powdery mildew pathogen and sampled at 0, 12, 24, and 48 hpi for integrated transcriptomic, proteomic, and metabolomic analyses. Quantitative PCR and CRISPR/Cas9-mediated gene editing were further used to evaluate the candidate susceptibility gene LbMLO6. Infection induced extensive molecular reprogramming, yielding 1299 differentially expressed genes with sustained changes, 75 DAPs shared across all three time points, and 127 shared differentially accumulated metabolites. These molecules were mainly enriched in plant–pathogen interaction, plant hormone signal transduction, MAPK signaling, phenylpropanoid biosynthesis, and α-linolenic acid metabolism pathways, suggesting coordinated roles for Ca2+ signaling, reactive oxygen species, defense-related proteins, and secondary metabolism in the host response. Integrated multi-omics analysis identified LbMLO6 as a candidate gene associated with powdery mildew susceptibility, its expression was markedly induced from 24 hpi onward. CRISPR/Cas9-edited LbMLO6 lines showed reduced fungal development compared with the control, particularly during the early stages of infection. These findings provide preliminary evidence that LbMLO6 may be associated with powdery mildew susceptibility in L. barbarum.
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BibTeX
@article{xu2026integrated,
title = {{Integrated Multi-Omics Analysis of the Response of Lycium barbarum ‘Ningqi-1’ to Powdery Mildew Infection and Functional Characterization of LbMLO6}},
author = {Wendi Xu and Lan Luo and Jiaqi Wang and Ruirui Ren and Jun Zhou and Cuiping Wang and Ken Qin and Yanlong Li and Yunxiang Li and Xin Zhang and Jing Wang and Binbin Si and Linyuan Duan and Guoli Dai},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27219412},
url = {https://www.mdpi.com/1422-0067/27/21/9412},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Integrated Multi-Omics Analysis of the Response of Lycium barbarum ‘Ningqi-1’ to Powdery Mildew Infection and Functional Characterization of LbMLO6
AU - Wendi Xu
AU - Lan Luo
AU - Jiaqi Wang
AU - Ruirui Ren
AU - Jun Zhou
AU - Cuiping Wang
AU - Ken Qin
AU - Yanlong Li
AU - Yunxiang Li
AU - Xin Zhang
AU - Jing Wang
AU - Binbin Si
AU - Linyuan Duan
AU - Guoli Dai
PY - 2026
DA - 2026/10/22
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27219412
UR - https://www.mdpi.com/1422-0067/27/21/9412
AB - Powdery mildew severely constrains the production of Lycium barbarum L., yet the molecular mechanisms underlying its response to infection remain poorly understood. Here, ‘Ningqi-1’ plants were inoculated with the powdery mildew pathogen and sampled at 0, 12, 24, and 48 hpi for integrated transcriptomic, proteomic, and metabolomic analyses. Quantitative PCR and CRISPR/Cas9-mediated gene editing were further used to evaluate the candidate susceptibility gene LbMLO6. Infection induced extensive molecular reprogramming, yielding 1299 differentially expressed genes with sustained changes, 75 DAPs shared across all three time points, and 127 shared differentially accumulated metabolites. These molecules were mainly enriched in plant–pathogen interaction, plant hormone signal transduction, MAPK signaling, phenylpropanoid biosynthesis, and α-linolenic acid metabolism pathways, suggesting coordinated roles for Ca2+ signaling, reactive oxygen species, defense-related proteins, and secondary metabolism in the host response. Integrated multi-omics analysis identified LbMLO6 as a candidate gene associated with powdery mildew susceptibility, its expression was markedly induced from 24 hpi onward. CRISPR/Cas9-edited LbMLO6 lines showed reduced fungal development compared with the control, particularly during the early stages of infection. These findings provide preliminary evidence that LbMLO6 may be associated with powdery mildew susceptibility in L. barbarum.
SN - 1422-0067
ER -