Association between the ACTN3 R577X polymorphism and skeletal muscle mass: a systematic review and meta-analysis.
- 1Graduate School of Health and Sport Science, Nippon Sport Science University, Tokyo, Japan. [email protected].
- 2Research Institute for Sport Science, Nippon Sport Science University, Tokyo, Japan.
- 3Graduate School of Health and Sport Science, Nippon Sport Science University, Tokyo, Japan.
Abstract
Background
The ACTN3 R577X polymorphism is one of the genetic factors influencing skeletal muscle mass. Although several studies have reported that the X allele of the ACTN3 R577X polymorphism is associated with reduced muscle mass, others have found no significant relationship between genotype and muscle mass. These inconsistencies may be explained by differences in background characteristics of the study populations, such as sex, age, ethnicity, and measurement site. This systematic review and meta-analysis aimed to clarify the association between the ACTN3 R577X polymorphism and skeletal muscle mass. In addition, we investigated whether the effect of the ACTN3 gene is modified by population background.Main body
A total of 34 cohorts (9,496 participants) derived from 24 studies were included in the meta-analysis. In the overall analysis under the dominant model for the X allele (RR vs. RX + XX), the ACTN3 genotype showed a significant effect on skeletal muscle mass (SMD [95% CI] = - 0.08 [- 0.15 to - 0.02], p = 0.014), with the RX + XX group exhibiting lower skeletal muscle mass compared with the RR group. In contrast, the recessive model (RR + RX vs. XX) did not yield a statistically significant effect (SMD [95% CI] = - 0.02 [- 0.12 to 0.07], p = 0.616). Under the additive model, a trend toward an effect was observed (per-allele; β [95% CI] = - 0.05 [- 0.10 to 0.01], p = 0.078). Moderate heterogeneity was observed across all models (dominant: I² = 38.8%; recessive: I² = 55.8%; additive: I² = 52.5%). No significant subgroup effects were observed for sex, age, or ethnicity. Meta-regression for measurement site indicated that the association became more pronounced as lower-limb specificity increased, although residual heterogeneity remained.Conclusion
The ACTN3 R577X polymorphism showed a small association with skeletal muscle mass under the X-allele dominant model, suggesting that this association may be detectable even in heterozygous individuals. Although measurement site may modulate this association, none of the examined factors substantially explained the observed heterogeneity when considered individually. The remaining variability may reflect combinations of background characteristics that were not fully captured by single-factor analyses. Further studies accounting for background characteristics are warranted to clarify the genetic contribution of ACTN3 R577X to skeletal muscle mass.
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BibTeX
@article{hattori2026association,
title = {{Association between the ACTN3 R577X polymorphism and skeletal muscle mass: a systematic review and meta-analysis.}},
author = {Sakura Hattori and Mika Kamiya-Saito and Naoki Kikuchi and Koichi Nakazato},
journal = {Journal of physiological anthropology},
year = {2026},
month = jul,
doi = {10.1186/s40101-026-00439-5},
url = {https://europepmc.org/article/MED/42907881},
note = {CC BY},
}
RIS
TY - JOUR
TI - Association between the ACTN3 R577X polymorphism and skeletal muscle mass: a systematic review and meta-analysis.
AU - Sakura Hattori
AU - Mika Kamiya-Saito
AU - Naoki Kikuchi
AU - Koichi Nakazato
PY - 2026
DA - 2026/07/27
JO - Journal of physiological anthropology
DO - 10.1186/s40101-026-00439-5
UR - https://europepmc.org/article/MED/42907881
AB - Background The ACTN3 R577X polymorphism is one of the genetic factors influencing skeletal muscle mass. Although several studies have reported that the X allele of the ACTN3 R577X polymorphism is associated with reduced muscle mass, others have found no significant relationship between genotype and muscle mass. These inconsistencies may be explained by differences in background characteristics of the study populations, such as sex, age, ethnicity, and measurement site. This systematic review and meta-analysis aimed to clarify the association between the ACTN3 R577X polymorphism and skeletal muscle mass. In addition, we investigated whether the effect of the ACTN3 gene is modified by population background.Main body A total of 34 cohorts (9,496 participants) derived from 24 studies were included in the meta-analysis. In the overall analysis under the dominant model for the X allele (RR vs. RX + XX), the ACTN3 genotype showed a significant effect on skeletal muscle mass (SMD [95% CI] = - 0.08 [- 0.15 to - 0.02], p = 0.014), with the RX + XX group exhibiting lower skeletal muscle mass compared with the RR group. In contrast, the recessive model (RR + RX vs. XX) did not yield a statistically significant effect (SMD [95% CI] = - 0.02 [- 0.12 to 0.07], p = 0.616). Under the additive model, a trend toward an effect was observed (per-allele; β [95% CI] = - 0.05 [- 0.10 to 0.01], p = 0.078). Moderate heterogeneity was observed across all models (dominant: I² = 38.8%; recessive: I² = 55.8%; additive: I² = 52.5%). No significant subgroup effects were observed for sex, age, or ethnicity. Meta-regression for measurement site indicated that the association became more pronounced as lower-limb specificity increased, although residual heterogeneity remained.Conclusion The ACTN3 R577X polymorphism showed a small association with skeletal muscle mass under the X-allele dominant model, suggesting that this association may be detectable even in heterozygous individuals. Although measurement site may modulate this association, none of the examined factors substantially explained the observed heterogeneity when considered individually. The remaining variability may reflect combinations of background characteristics that were not fully captured by single-factor analyses. Further studies accounting for background characteristics are warranted to clarify the genetic contribution of ACTN3 R577X to skeletal muscle mass.
SN - 1880-6791
SN - 1880-6805
ER -