Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 34 min ago
PreprintCC BYbioRxiv

Auxin-inducible degron-mediated protein depletion in Diplonema papillatum

  • G. Clifford
  • B. Akiyoshi1
  1. 1University of Edinburgh
Read full textPDFDOICite

Abstract

Diplonemids are highly abundant unicellular flagellates that are evolutionarily divergent from traditional model eukaryotes. Diplonema papillatum is a model diplonemid for which several molecular tools have recently been established. However, no method for conditional protein depletion was available. Here, we have adapted an auxin-inducible degron (AID) system in D. papillatum. Depletion of the KIN-A motor protein (a glycomonad-specific component of the chromosomal passenger complex) causes defects in chromosome segregation and cell proliferation. The AID system also successfully depleted a divergent Mad1 protein that localizes at nuclear pores, and a flagellar component, CFAP20. Our study demonstrates the power of the AID system to perform loss-of-function studies in D. papillatum.

Versions

Related

  1. Diagnostic accuracy of Mycobacterium tuberculosis antigen-induced interferon-γ-inducible protein 10 for latent tuberculosis infection among high-risk groups: a systematic review and meta-analysis
    Lan Huang, Yong Hu, Qian Mao +2
    Frontiers in immunologyPeer-reviewedCC BYFull textImmunologyEurope PMC
  2. Chaperone protein DnaK-mediated regulation of spore coat assembly in Bacillus subtilis
    Kangyi Mu, Chunrong Ling, Tianlin Cui +4
    ISciencePeer-reviewedCC BY-NC-NDFull textGenetics & genomicsEurope PMCCrossref
  3. Editorial Note: C4b Binding Protein Binds to CD154 Preventing CD40 Mediated Cholangiocyte Apoptosis: A Novel Link between Complement and Epithelial Cell Survival
    The PLOS One Editors
    PLOS OnePeer-reviewedCC BY 4.0Full textImmunologyPLOS
  4. Regulation of Anxiety and Depression by Mitochondrial Translocator Protein-Mediated Steroidogenesis: the Role of Neurons
    Anna M. Barron, Makoto Higuchi, Satoko Hattori +3
    Molecular NeurobiologyPeer-reviewedLicence not stated43 citedNeuroscienceHAL
  5. SIR2-mediated filament assembly activates the NLR-like protein Avs5 for bacterial antiviral defense
    Chen Wang, Jiale Xie, Zhangfei Shen +5
    Cell ReportsPeer-reviewedCC BY-NC-ND 4.0ImmunologyCrossref

Cite

BibTeXRIS
BibTeX
@misc{clifford2026auxininducible,
  title = {{Auxin-inducible degron-mediated protein depletion in Diplonema papillatum}},
  author = {G. Clifford and B. Akiyoshi},
  howpublished = {bioRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.11.751042},
  url = {https://www.biorxiv.org/content/10.64898/2026.09.11.751042v1},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - Auxin-inducible degron-mediated protein depletion in Diplonema papillatum
AU  - G. Clifford
AU  - B. Akiyoshi
PY  - 2026
DA  - 2026/09/11
PB  - bioRxiv
DO  - 10.64898/2026.09.11.751042
UR  - https://www.biorxiv.org/content/10.64898/2026.09.11.751042v1
AB  - Diplonemids are highly abundant unicellular flagellates that are evolutionarily divergent from traditional model eukaryotes. Diplonema papillatum is a model diplonemid for which several molecular tools have recently been established. However, no method for conditional protein depletion was available. Here, we have adapted an auxin-inducible degron (AID) system in D. papillatum. Depletion of the KIN-A motor protein (a glycomonad-specific component of the chromosomal passenger complex) causes defects in chromosome segregation and cell proliferation. The AID system also successfully depleted a divergent Mad1 protein that localizes at nuclear pores, and a flagellar component, CFAP20. Our study demonstrates the power of the AID system to perform loss-of-function studies in D. papillatum.
ER  -