Fas receptor binding is a key determinant of the enhanced aggressiveness of the novel recombinant Echovirus 11.
6 affiliations
- 1Division of Microbiology and Immunology, Emory Vaccine Centre, Emory National Primate Research Centre, Emory University, Atlanta, USA.
- 2Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
- 3Department of Science and Bio-Technology, Università Campus Bio-Medico di Roma, Roma, Italy.
- 4Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
- 5Institute of Microbiology, ASST-Spedali Civili, Brescia, Italy.
- 6Department of Pathology and Laboratory Medicine, Emory University, Atlanta, USA.
Abstract
A novel recombinant strain of Echovirus 11 has recently emerged worldwide and has been associated with increased pathogenicity, particularly causing severe disease in neonates. The enhanced virulence of this variant may be linked to its recombinant nature and the accumulation of mutations in capsid proteins.In this study, we investigated the relationship between genetic variations and virulence using a clinical isolate of the dominant Echovirus 11 D5 genotype, in comparison with the prototype strain. In vitro characterization in A549 human lung epithelial cells revealed that the clinical isolate exhibits significantly greater cytopathic effects than the prototype, as evidenced by increased lytic replication, reduced cell viability, and higher viral loads accompanied by enhanced release of infectious particles. The isolated virus was found to directly bind and activate the Fas receptor as demonstrated by surface plasmon resonance and cross-linking mass spectrometry analyses. This was found to enhance productive viral replication and facilitate the efficient release of newly assembled virions from infected cells, thereby promoting rapid viral dissemination. Collectively, these findings elucidate the mechanism underlying the increased aggressiveness of this emerging Echovirus 11 variant and underscore the importance of heightened surveillance and awareness of its potential clinical impact.
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BibTeX
@article{sclavi2026receptor,
title = {{Fas receptor binding is a key determinant of the enhanced aggressiveness of the novel recombinant Echovirus 11.}},
author = {Leonardo Sclavi and Giulia Venneri and Antonella Bugatti and Marta Giovanetti and Serena Messali and Cheryl L Maier and Guido Silvestri and Francesca Caccuri and Arnaldo Caruso},
journal = {Emerging microbes \& infections},
year = {2026},
month = oct,
doi = {10.1080/22221751.2026.2746750},
url = {https://europepmc.org/article/MED/42896099},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - Fas receptor binding is a key determinant of the enhanced aggressiveness of the novel recombinant Echovirus 11.
AU - Leonardo Sclavi
AU - Giulia Venneri
AU - Antonella Bugatti
AU - Marta Giovanetti
AU - Serena Messali
AU - Cheryl L Maier
AU - Guido Silvestri
AU - Francesca Caccuri
AU - Arnaldo Caruso
PY - 2026
DA - 2026/10/19
JO - Emerging microbes & infections
DO - 10.1080/22221751.2026.2746750
UR - https://europepmc.org/article/MED/42896099
AB - A novel recombinant strain of Echovirus 11 has recently emerged worldwide and has been associated with increased pathogenicity, particularly causing severe disease in neonates. The enhanced virulence of this variant may be linked to its recombinant nature and the accumulation of mutations in capsid proteins.In this study, we investigated the relationship between genetic variations and virulence using a clinical isolate of the dominant Echovirus 11 D5 genotype, in comparison with the prototype strain. In vitro characterization in A549 human lung epithelial cells revealed that the clinical isolate exhibits significantly greater cytopathic effects than the prototype, as evidenced by increased lytic replication, reduced cell viability, and higher viral loads accompanied by enhanced release of infectious particles. The isolated virus was found to directly bind and activate the Fas receptor as demonstrated by surface plasmon resonance and cross-linking mass spectrometry analyses. This was found to enhance productive viral replication and facilitate the efficient release of newly assembled virions from infected cells, thereby promoting rapid viral dissemination. Collectively, these findings elucidate the mechanism underlying the increased aggressiveness of this emerging Echovirus 11 variant and underscore the importance of heightened surveillance and awareness of its potential clinical impact.
SN - 2222-1751
ER -