Neuronal excitability coordinates the timing of input/output circuit assembly
- 1Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM,Université PSL, 75005 Paris, France
Abstract
How neurons coordinate the timing of migration, differentiation and synaptic integration with their specific inputs and outputs remains poorly understood. Here we show that granule cells, the brain's most numerous neuronal population, establish immature synaptic contacts on their output targets, the cerebellar Purkinje cells, well before completing their radial migration and becoming innervated by their mossy fiber inputs. This happens at a specific stage when granule cells reach the molecular layer and begin expressing presynaptic markers and a specific NMDA receptor subtype. Chemogenetic silencing of these migrating, differentiating granule cells shows that their excitability is required for output synapse maturation. Thus neuronal activity coordinates the timing of input and output synapse development. This mechanism might underlie GRIN2B-related pathologies that include autism spectrum disorders.
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BibTeX
@misc{urrietachavez2026neuronal,
title = {{Neuronal excitability coordinates the timing of input/output circuit assembly}},
author = {B. Urrieta-Chavez and C. Tran and B. Garcia-Hernandez and S. Domenget and F. Selimi},
howpublished = {bioRxiv},
year = {2026},
month = jul,
doi = {10.64898/2026.07.27.740696},
url = {https://www.biorxiv.org/content/10.64898/2026.07.27.740696v2},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Neuronal excitability coordinates the timing of input/output circuit assembly
AU - B. Urrieta-Chavez
AU - C. Tran
AU - B. Garcia-Hernandez
AU - S. Domenget
AU - F. Selimi
PY - 2026
DA - 2026/07/27
PB - bioRxiv
DO - 10.64898/2026.07.27.740696
UR - https://www.biorxiv.org/content/10.64898/2026.07.27.740696v2
AB - How neurons coordinate the timing of migration, differentiation and synaptic integration with their specific inputs and outputs remains poorly understood. Here we show that granule cells, the brain's most numerous neuronal population, establish immature synaptic contacts on their output targets, the cerebellar Purkinje cells, well before completing their radial migration and becoming innervated by their mossy fiber inputs. This happens at a specific stage when granule cells reach the molecular layer and begin expressing presynaptic markers and a specific NMDA receptor subtype. Chemogenetic silencing of these migrating, differentiating granule cells shows that their excitability is required for output synapse maturation. Thus neuronal activity coordinates the timing of input and output synapse development. This mechanism might underlie GRIN2B-related pathologies that include autism spectrum disorders.
ER -