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PreprintCC BYChemRxiv

Artificial Cells with a Cytoskeleton from Synthetic Self-Replicating Molecules

  • Paula De Dios Andres1,2,3
  • Lukas C. Herold4,5
  • Kirill Mikhailov4,5
  • Sijbren Otto4,5
  • Brigitte Städler1,2,3
5 affiliations
  1. 1Interdisciplinary Nanoscience Center (iNANO
  2. 2Aarhus University
  3. 3Department of Chemisty
  4. 4Centre for Systems Chemistry Stratingh Institute
  5. 5University of Groningen
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Abstract

Living cells couple molecular replication and cytoskeletal assembly to changes in shape and mechanical stability, but reconstructing this relationship in synthetic compartments remains challenging. Here, we confine self-replicating supramolecular fibers within giant polymer lipid hybrid vesicle-based artificial cells. Seeded replicator growth produces luminal fiber networks and deforms the surrounding membrane into spindle-like morphologies, with fiber and membrane interactions controlled by membrane composition and fiber glycation. Covalent crosslinking of the fiber network within the lumen enables the artificial cells to withstand cellular aggregation during prolonged co-culture. The stabilized artificial cells remain incorporated within HepaRG cell-based microtissues for 28 days while the surrounding cells differentiate into hepatocyte- and biliary-like phenotypes. These results establish a chemical route by which assembly-driven replicators within a compartment can generate cytoskeletal

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Cite

BibTeXRIS
BibTeX
@misc{andres2026artificial,
  title = {{Artificial Cells with a Cytoskeleton from Synthetic Self-Replicating Molecules}},
  author = {Paula De Dios Andres and Lukas C. Herold and Kirill Mikhailov and Sijbren Otto and Brigitte Städler},
  howpublished = {ChemRxiv},
  year = {2026},
  month = oct,
  doi = {10.26434/chemrxiv.15010885/v1},
  url = {https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010885/v1},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - Artificial Cells with a Cytoskeleton from Synthetic Self-Replicating Molecules
AU  - Paula De Dios Andres
AU  - Lukas C. Herold
AU  - Kirill Mikhailov
AU  - Sijbren Otto
AU  - Brigitte Städler
PY  - 2026
DA  - 2026/10/22
PB  - ChemRxiv
DO  - 10.26434/chemrxiv.15010885/v1
UR  - https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010885/v1
AB  - Living cells couple molecular replication and cytoskeletal assembly to changes in shape and mechanical stability, but reconstructing this relationship in synthetic compartments remains challenging. Here, we confine self-replicating supramolecular fibers within giant polymer lipid hybrid vesicle-based artificial cells. Seeded replicator growth produces luminal fiber networks and deforms the surrounding membrane into spindle-like morphologies, with fiber and membrane interactions controlled by membrane composition and fiber glycation. Covalent crosslinking of the fiber network within the lumen enables the artificial cells to withstand cellular aggregation during prolonged co-culture. The stabilized artificial cells remain incorporated within HepaRG cell-based microtissues for 28 days while the surrounding cells differentiate into hepatocyte- and biliary-like phenotypes. These results establish a chemical route by which assembly-driven replicators within a compartment can generate cytoskeletal
ER  -