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Peer-reviewedCC BY-NC 4.0The New Phytologist

microRNA‐mediated control of cell fate specification and patterning in Marchantia polymorpha

  • Adolfo Aguilar‐Cruz1
  • Eduardo Flores‐Sandoval2,3
  • Ye Xu4
  • Yu Yu5,6
  • Ximena Gutiérrez‐Ramos7
  • Cristian Y. González‐Rangel1
  • Omar Oltehua‐López1,8
  • Ana E. Dorantes‐Acosta1
  • Joshua T. Trujillo9
  • Hirotaka Kato10
  • Kimitsune Ishizaki11
  • Rebecca A. Mosher8,12
6 more authors
  • Liam Dolan13
  • Xuemei Chen6
  • Daniel Grimanelli14
  • Jim Haseloff15
  • John L. Bowman2,3
  • Mario A. Arteaga‐Vazquez1
15 affiliations
  1. 1Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana Avenida de las Culturas Veracruzanas 101, Col. Emiliano Zapata C.P. 91090 Xalapa Veracruz México
  2. 2School of Biological Sciences Monash University Melbourne Vic. 3800 Australia
  3. 3ARC Centre of Excellence for Plant Success in Nature and Agriculture Monash University Melbourne Vic. 3800 Australia
  4. 4Department of Botany and Plant Science University of California Riverside CA 92521 USA
  5. 5State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking‐Tsinghua Center for Life Sciences Peking University Beijing 100871 China
  6. 6Beijing Advanced Center of RNA Biology (BEACON), School of Life Sciences Peking University Beijing 100871 China
  7. 7Department of Entomology University of Maryland College Park MD 20742 USA
  8. 8Department of Biotechnology Universidad Autónoma Metropolitana‐Iztapalapa Av. Ferrocarril San Rafael Atlixco 186, Col. Leyes de Reforma 1A Sección, Iztapalapa, CDMX Mexico City 09310 Mexico
  9. 9The School of Plant Sciences The University of Arizona Tucson AZ 85721 USA
  10. 10Graduate School of Science and Engineering Ehime University Matsuyama 790‐8577 Japan
  11. 11Graduate School of Science Kobe University Hyogo 657‐8501 Japan
  12. 12Department of Biology University of Oxford Oxford OX1 3EL UK
  13. 13Gregor Mendel Institute Dr. Bohr‐Gasse, 3 Vienna 1030 Austria
  14. 14Institut de Recherche pour le Développement 911 Avenue Agropolis 34394 Montpellier France
  15. 15Department of Plant Sciences University of Cambridge Downing St. Cambridge CB2 3EA UK
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Abstract

MicroRNAs (miRNAs) are small non‐coding RNA molecules essential for growth and development in eukaryotes. In plants, the master gene DICER‐LIKE 1 (DCL1) catalyzes the biogenesis of miRNAs by processing double‐stranded precursors that give rise to mature miRNAs. We sought to understand the function and evolution of microRNAs using Marchantia polymorpha, a model bryophyte that allows comparative approaches to infer characteristics of the ancestral land plant.

We functionally characterized loss‐of‐function mutants of MpDCL1 a generated by means of CRISPR‐Cas9‐mediated genomic edition and the miR166/CLASS III HD‐ZIP regulatory circuit in Marchantia polymorpha.

We report that MpDCL1a is required for the biogenesis of miRNAs and uncovered a central role for miR166/Homeodomain Zipper Class III‐regulated auxin synthesis in the specification of cell identity, patterning, meristem function, laminar expansion, and the development of the body in the last common ancestor of the bryophytes and vascular plants.

Our findings indicate that DCL1, Class III HD‐ZIP, miR166, and auxin functioned in the development of the body of the last common ancestor of extant land plants and provide a novel working framework to interrogate the basic principles of cell specification and patterning in plants.

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Cite

BibTeXRIS
BibTeX
@article{aguilarcruz2026micrornamediated,
  title = {{microRNA‐mediated control of cell fate specification and patterning in Marchantia polymorpha}},
  author = {Adolfo Aguilar‐Cruz and Eduardo Flores‐Sandoval and Ye Xu and Yu Yu and Ximena Gutiérrez‐Ramos and Cristian Y. González‐Rangel and Omar Oltehua‐López and Ana E. Dorantes‐Acosta and Joshua T. Trujillo and Hirotaka Kato and Kimitsune Ishizaki and Rebecca A. Mosher and Liam Dolan and Xuemei Chen and Daniel Grimanelli and Jim Haseloff and John L. Bowman and Mario A. Arteaga‐Vazquez},
  journal = {The New Phytologist},
  year = {2026},
  month = aug,
  doi = {10.1111/nph.71487},
  url = {https://doi.org/10.1111/nph.71487},
  note = {CC BY-NC 4.0},
}
RIS
TY  - JOUR
TI  - microRNA‐mediated control of cell fate specification and patterning in Marchantia polymorpha
AU  - Adolfo Aguilar‐Cruz
AU  - Eduardo Flores‐Sandoval
AU  - Ye Xu
AU  - Yu Yu
AU  - Ximena Gutiérrez‐Ramos
AU  - Cristian Y. González‐Rangel
AU  - Omar Oltehua‐López
AU  - Ana E. Dorantes‐Acosta
AU  - Joshua T. Trujillo
AU  - Hirotaka Kato
AU  - Kimitsune Ishizaki
AU  - Rebecca A. Mosher
AU  - Liam Dolan
AU  - Xuemei Chen
AU  - Daniel Grimanelli
AU  - Jim Haseloff
AU  - John L. Bowman
AU  - Mario A. Arteaga‐Vazquez
PY  - 2026
DA  - 2026/08/09
JO  - The New Phytologist
DO  - 10.1111/nph.71487
UR  - https://doi.org/10.1111/nph.71487
AB  - MicroRNAs (miRNAs) are small non‐coding RNA molecules essential for growth and development in eukaryotes. In plants, the master gene DICER‐LIKE 1 (DCL1) catalyzes the biogenesis of miRNAs by processing double‐stranded precursors that give rise to mature miRNAs. We sought to understand the function and evolution of microRNAs using Marchantia polymorpha, a model bryophyte that allows comparative approaches to infer characteristics of the ancestral land plant.  We functionally characterized loss‐of‐function mutants of MpDCL1 a generated by means of CRISPR‐Cas9‐mediated genomic edition and the miR166/CLASS III HD‐ZIP regulatory circuit in Marchantia polymorpha.  We report that MpDCL1a is required for the biogenesis of miRNAs and uncovered a central role for miR166/Homeodomain Zipper Class III‐regulated auxin synthesis in the specification of cell identity, patterning, meristem function, laminar expansion, and the development of the body in the last common ancestor of the bryophytes and vascular plants.  Our findings indicate that DCL1, Class III HD‐ZIP, miR166, and auxin functioned in the development of the body of the last common ancestor of extant land plants and provide a novel working framework to interrogate the basic principles of cell specification and patterning in plants.
SN  - 0028-646X
SN  - 1469-8137
ER  -