Integrated phenotypic and genome-wide characterization of Ralstonia solanacearum strain R1 of tobacco.
6 affiliations
- 1Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, Yunnan, 650224, China.
- 2Jiangxi Provincial Key Laboratory of Plantation and High Valued Utilization of Specialty Fruit Tree and Tea, Institute of Resources and Environment, Jiangxi Academy of Sciences, Nanchang, Jiangxi, 330096, China.
- 3College of Land and Environment, Shenyang Agictlhral University, Shenyang, Liaoning, 110866, China.
- 4Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China. [email protected].
- 5Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming, Yunnan, 650224, China. [email protected].
- 6Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, Yunnan, 650224, China. [email protected].
Abstract
Background
Tobacco bacterial wilt, a devastating soil-borne disease caused by the Ralstonia solanacearum species complex (RSSC), poses a severe threat to tobacco production.Results
In this study, a predominant Ralstonia solanacearum strain, designated R1, was isolated from a typical diseased tobacco field in Zhangjiajie, Hunan Province, China. The strain was characterized through a combination of morphological observation, growth curve analysis, and phenotypic profiling using Biolog GEN III MicroPlates. These assays collectively revealed its key morphological features and physiological and biochemical properties. Whole-genome sequencing revealed that the R1 genome is 4.92 Mb in size, with a GC content of 66.80%, comprising a circular chromosome and a 1.03-Mb megaplasmid. Comparative genomic and phylogenomic analyses further assigned strain R1 to Phylotype I, which is characteristic of Asian-origin strains. Genome analysis identified 400 potential virulence factors, 233 antibiotic resistance genes (ARGs), and 7 predicted biosynthetic gene clusters (BGCs) for secondary metabolites. Notably, two non-ribosomal peptide synthetase (NRPS)-related clusters exhibited high homology to known gene clusters responsible for synthesizing the antimicrobial compounds micacocidin and rhizomide.Conclusions
This study presents the first integrated genomic and phenotypic analysis of an R. solanacearum strain from the Zhangjiajie ecological region in western Hunan. The findings provide key insights into the adaptive evolution and pathogenic mechanisms of the local pathogen, as well as crucial theoretical foundations and genetic targets for breeding region-specific, disease-resistant tobacco varieties, novel green control strategies based on quorum-sensing interference, and science-based guidelines for antibiotic use in agriculture.
Related
- Bark extracts of Litsea glutinosa induces systemic resistance for controlling wilt of tomato caused by Ralstonia solanacearum
- Contrasting gene gain and loss shape metabolic diversification in the Ralstonia solanacearum species complex
- Riboflavin Primes Pepper Resistance to Ralstonia solanacearum Through Coordinated Antioxidant Responses and Lignin Deposition
- Assembly processes and network structure of rhizosphere fungal communities in healthy and Ralstonia solanacearum-infected tomato plants
- Ethnobotanical survey and antibiofilm evaluation of medicinal plants used for the control of tomato bacterial wilt caused by Ralstonia solanacearum
BibTeX
@article{he2026integrated,
title = {{Integrated phenotypic and genome-wide characterization of Ralstonia solanacearum strain R1 of tobacco.}},
author = {Yanmei He and Dongliang Yao and Shipei Zu and Yi Liu and Junzhong Zhang},
journal = {BMC plant biology},
year = {2026},
month = jul,
doi = {10.1186/s12870-026-09505-7},
url = {https://europepmc.org/article/MED/42928122},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Integrated phenotypic and genome-wide characterization of Ralstonia solanacearum strain R1 of tobacco.
AU - Yanmei He
AU - Dongliang Yao
AU - Shipei Zu
AU - Yi Liu
AU - Junzhong Zhang
PY - 2026
DA - 2026/07/29
JO - BMC plant biology
DO - 10.1186/s12870-026-09505-7
UR - https://europepmc.org/article/MED/42928122
AB - Background Tobacco bacterial wilt, a devastating soil-borne disease caused by the Ralstonia solanacearum species complex (RSSC), poses a severe threat to tobacco production.Results In this study, a predominant Ralstonia solanacearum strain, designated R1, was isolated from a typical diseased tobacco field in Zhangjiajie, Hunan Province, China. The strain was characterized through a combination of morphological observation, growth curve analysis, and phenotypic profiling using Biolog GEN III MicroPlates. These assays collectively revealed its key morphological features and physiological and biochemical properties. Whole-genome sequencing revealed that the R1 genome is 4.92 Mb in size, with a GC content of 66.80%, comprising a circular chromosome and a 1.03-Mb megaplasmid. Comparative genomic and phylogenomic analyses further assigned strain R1 to Phylotype I, which is characteristic of Asian-origin strains. Genome analysis identified 400 potential virulence factors, 233 antibiotic resistance genes (ARGs), and 7 predicted biosynthetic gene clusters (BGCs) for secondary metabolites. Notably, two non-ribosomal peptide synthetase (NRPS)-related clusters exhibited high homology to known gene clusters responsible for synthesizing the antimicrobial compounds micacocidin and rhizomide.Conclusions This study presents the first integrated genomic and phenotypic analysis of an R. solanacearum strain from the Zhangjiajie ecological region in western Hunan. The findings provide key insights into the adaptive evolution and pathogenic mechanisms of the local pathogen, as well as crucial theoretical foundations and genetic targets for breeding region-specific, disease-resistant tobacco varieties, novel green control strategies based on quorum-sensing interference, and science-based guidelines for antibiotic use in agriculture.
SN - 1471-2229
ER -